Effect of Delay Separation and Short Term Storage of Serum on Thyroid Stimulating Hormone (TSH)

Basanta, G; Gupta, Seema; Gautam, Swotantra; Ashwini, K.; Das, BK Lal

doi:10.17352/ojtr.000003

Cited by 2 publications

(4 citation statements)

References 8 publications

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“…We found no difference across centrifugation timings in serum TSH concentration upto 24 hours after blood collection. Similar findings were also documented by Basanta et al 9 in a study conducted in Nepal in 2017. This study revealed that, TSH measurement might be applicable in large epidemiological studies, which require very long transportation time to reach samples to the…”

Section: Discussionsupporting

confidence: 90%

“…Sampling technique used was non-probability convenience sampling. Sample size was calculated using EPI info Statistical calculator by using frequency of pre analytical variables affecting thyroid assay published in article Basanta et al in 2017 9 . Individuals with history of chronic illness, obstetric event (i.e.…”

Section: Methodsmentioning

confidence: 99%

“…It was recommended that serum must be separated from plasma within 2 hours of sampling. Different analytes have different tolerance levels and many of them are stable for longer duration as well 9 .…”

Section: Introductionmentioning

confidence: 99%

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Effect of Preanalytical Variables on Serum Thyroid Stimulating Hormone Analysis by Chemiluminescence Method

Rehman

Asif

Malik

et al. 2021

PAFMJ

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Objective: To identify effect of pre-analytical variables on serum thyroid stimulating hormone. Study Design: Cross sectional study. Place and Duration of Study: Armed Forces Institute of Pathology (AFIP) Rawalpindi, Department of Chemical Pathology & Endocrinology, from Mar 2018 to Aug 2018. Methodology: Hundred subjects with ages ranging from 18 to 34 years, irrespective of gender, were randomly selected for this study. Five milliliters venous blood sample was collected from each subject in a serum separator and divided into two aliquots. First aliquot was centrifuged and analyzed immediately for TSH, while second aliquot was stored for 24 hours and was then analyzed. TSH was measured by third generation assay usingchemiluminescence technique on ADVIA Centaur® XP. Serum TSH levels were also analyzed twice daily; in the morning (0800 to 0900 hours) and afternoon (1400 to 1600 hours). Data was analyzed using SPSS version 24. Frequency and percentages were calculated for qualitative variables like gender and pre-analytical variables. Test of significance Mann-Whitney U-test was applied and p-value <0.05 was taken as significant. Results: Mean age of subjects was 23 ± 3.4 years. Change in circadian rhythm was observed in 17 (28%) males and 14 (36%) females. Statistically significant association was found between morning and evening TSH levels, while no change was observed in TSH level by early and late centrifugation of samples. Conclusion: TSH levels vary significantly between blood samples collected at different timings of the day from the same person. TSH is resistant to degradation, immunologically stable, and reasonably insensitive to potential problems associated with routine specimen handling, when measured by immunoassay technique. Therefore, it is helpful in large epidemiological studies and small size laboratory, which require long transportation time and storage.

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Section: Discussionsupporting

confidence: 90%

Section: Methodsmentioning

confidence: 99%

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Effect of Preanalytical Variables on Serum Thyroid Stimulating Hormone Analysis by Chemiluminescence Method

Rehman

Asif

Malik

et al. 2021

PAFMJ

View full text Add to dashboard Cite

show abstract

“…In contrast, a more recent study found that TSH assays performed on sera from fifteen patients that were immediately assayed, then assayed after standing at room temperature for 24h, and then assayed after storage at 4 o C for one week, all gave values that were statistically insignificantly different 8 . An early comprehensive study of thyroid function test stabilities found significant differences in all thyroid function tests depending on ambient temperature 9 .…”

Section: Introductionmentioning

confidence: 80%

Stability of Thyroid Function Test Analytes Whose Serum Levels Are Determined by Immunoassay

Christian

Minkowitz²,

Gabutan³

et al. 2023

MRAJ

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We have performed stability studies on thyroid function tests, i.e., T4, T3, free T4, free T3 and thyroid stimulating hormone (TSH), over a five-day period on the sera of ten patients. We find that the levels for each analyte were stable; the mean coefficients of variation (CV) ranged from 3.2 % for TSH to 8.7% for T4. ANOVA statistical analysis of group means for each analyte over the five-day period indicated that there was no statistically significant difference in these means, confirming the reproducibility of values. On the other hand, the CVs are significantly larger than those found in our prior stability studies for other critical analytes such as electrolytes (sodium, potassium, chloride, calcium). In addition, significant variations in values over the five-day period occurred for T4 which showed the largest variations in values of the five analytes studied. In one patient, the values ranged from a low of <10 ug/dL to over 14 ug/dL over the five-day period. A two-tailed t test comparing the mean CV for TSH with the mean CVs of the other four analytes showed that the mean CV for TSH was statistically significantly lower than that for the other four analytes indicating that the assay for TSH yield the most precise results. These results parallel those obtained in our prior electrolyte study that showed very low CVs for sodium, potassium and chloride and a significantly higher CV for calcium. Since approximately half of total serum calcium is bound to albumin and since very high percentages of T4 and T3 (≥99%) are bound to thyroid binding globulin and albumin, protein binding of these analytes may introduce a possible source of assay imprecision.

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Effect of Delay Separation and Short Term Storage of Serum on Thyroid Stimulating Hormone (TSH)

Cited by 2 publications

References 8 publications

Effect of Preanalytical Variables on Serum Thyroid Stimulating Hormone Analysis by Chemiluminescence Method

Effect of Preanalytical Variables on Serum Thyroid Stimulating Hormone Analysis by Chemiluminescence Method

Stability of Thyroid Function Test Analytes Whose Serum Levels Are Determined by Immunoassay

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