Effect of dehydroepiandrosterone (DHEA) treatment on oxidative energy metabolism in rat liver and brain mitochondria. A dose–response study

Patel, Minal; Katyare, Surendra S.

doi:10.1016/j.clinbiochem.2006.08.014

Cited by 22 publications

(20 citation statements)

References 36 publications

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“…The animals received 0.2 or 1.0 mg DHEA/Kg body weight subcutaneously (s.c.) for 7 consecutive days. This dose regimen was decided on the basis of our earlier observation (Patel and Katyare 2007). Suspension of DHEA was prepared fresh in saline prior to injection.…”

Section: Chemicalmentioning

confidence: 99%

“…Measurements of oxidative phosphorylation were carried out at 25°C using a Clark-type oxygen electrode as described previously (Katewa and Katyare 2004;Patel and Katyare 2007). State 3 respiration rates initiated by the addition of 80-200 n moles of ADP and state 4 rates ensuing after its depletion were recorded.…”

Section: Oxidative Phosphorylationmentioning

confidence: 99%

“…Glutamate dehydrogenase (GDH), malate dehydrogenase (MDH) and succinate DCIP reductase (SDR) activities were determined by the procedures described earlier (Katewa and Katyare 2004;Patel and Katyare 2007).…”

Section: Assay Of Dehydrogenasesmentioning

confidence: 99%

“…Isolation of liver mitochondria was essentially according to the procedures described previously with some modifications (Katewa and Katyare 2004;Patel and Katyare 2007) The mitochondria were sedimented by centrifugation of the nuclei-free supernatant at 7000×g for 10 min and were washed once by suspending in the isolation medium (250 mM sucrose containing 5 mM MOPS and 1 mM EDTA all at pH 7.4 and 0.25 mg BSA/ml of isolation medium) and resedimenting. Finally the pellet was suspended in the isolation medium to give a protein concentration in the range of 25-30 mg/ml.…”

Section: Isolation Of Mitochondriamentioning

confidence: 99%

“…It is well recognized that the respiratory activity of mitochondria from different tissues declines with aging (Marcus et al 1982;Sastre et al 1996;Nakahara et al 1998;Navarro and Boveris 2004). Earlier studies from our laboratory have shown that DHEA treatment stimulated respiratory functions in liver and brain mitochondria from young rats (Patel and Katyare 2007).…”

Section: Introductionmentioning

confidence: 97%

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Stimulation of oxidative energy metabolism in liver mitochondria from old and young rats by treatment with dehydroepiandrosterone (DHEA). A comparative study

Patel

Modi

Katyare

2007

AGE

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Effects of treatment with DHEA (0.2 or 1.0 mg/kg body weight for 7 days) on oxidative energy metabolism of rat liver mitochondria from old (18-24 month old) and young (8-10 weeks old) male albino rats belonging to Charles-Foster strain were examined. Treatment with 1.0 mg DHEA resulted in increased body weights of the young rats without change in the liver weight. In the old animals the liver weight increased progressively with increasing dose of DHEA without affecting body weight. The state 3 respiration rates in liver mitochondria from old animals were, in general, lower than those in the young rats. The state 3 and state 4 respiration rates increased following DHEA treatment in dose-dependent manner bringing them close to values for young animals or beyond that with the effect being more pronounced at 1.0 mg dose. Treatment with DHEA also stimulated state 3 and state 4 respiration rates in young rats in dose-dependent manner. Contents of cytochrome aa(3), b and c + c(1) increased significantly in old animals in dose-dependent manner. In the young rats the lower dose (0.2 mg) of DHEA was more effective in bringing about a maximum increase in the contents of the cytochromes; the effect declined at the higher dose (1.0 mg). DHEA treatment also stimulated the mitochondrial ATPase activity in the old as well as in the young rats. The dehydrogenases activities were considerably low in the old rats compared to the values for the young animals. Treatment with DHEA stimulated dehydrogenases activities in old rats in dose-dependent manner bringing them close to values for the young animals or beyond. Treatment with lower dose (0.2 mg) of DHEA maximally stimulated dehydrogenases activities in young animals.

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Section: Chemicalmentioning

confidence: 99%

Section: Oxidative Phosphorylationmentioning

confidence: 99%

Section: Assay Of Dehydrogenasesmentioning

confidence: 99%

Section: Isolation Of Mitochondriamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

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Stimulation of oxidative energy metabolism in liver mitochondria from old and young rats by treatment with dehydroepiandrosterone (DHEA). A comparative study

Patel

Modi

Katyare

2007

AGE

Self Cite

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Adrenarche in comparative perspective

Campbell

2010

American J Hum Biol

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Compared to rats and macaque, in humans the energetic demands of brain development and increased production of adrenal androgens are divorced from the timing of lactation, while the timing of adrenarche and brain development are still associated. Thus the neuroprotective effects of DHEAS may protect synaptic plasticity in metabolically active parts of the brain starting approximately at the age of 7, promoting prolonged development of the human prefrontal cortex.

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Dehydroepiandrosterone Administration in Treating Medical and Neuropsychiatric Disorders

Ritsner

2008

Neuroactive Steroids in Brain Function, Behavior and Neuropsychiatric Disorders

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Effect of dehydroepiandrosterone (DHEA) treatment on oxidative energy metabolism in rat liver and brain mitochondria. A dose–response study

Cited by 22 publications

References 36 publications

Stimulation of oxidative energy metabolism in liver mitochondria from old and young rats by treatment with dehydroepiandrosterone (DHEA). A comparative study

Stimulation of oxidative energy metabolism in liver mitochondria from old and young rats by treatment with dehydroepiandrosterone (DHEA). A comparative study

Adrenarche in comparative perspective

Dehydroepiandrosterone Administration in Treating Medical and Neuropsychiatric Disorders

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