Effect of Daylength on Phenol Metabolism in the Leaves of Salvia occidentalis

Abstract

A method of phenol determination in plant leaves has been developed which is based on the in situ oxidation of these compounds in an atmosphere containing ammonia, followed by difference spectrophotometry. The The daylength affects phenol metabolism in the leaves of plants (4,7,(11)(12)(13)15). Attempts have been made to relate this to photoperiodic phenomena, e.g. flower induction (11,13,15). Such a study is hampered by a number of problems. The fully grown plant carries leaves of different age and different life history. Consequently, the leaves are often of different phenol content and composition. The presence of phenols in different cell compartments and their occurrence in both bound and unbound forms pose other difficulties. The classical method of phenol determination by extraction, chromatography, and spectrophotometry requires much time and fairly large amounts of material and is therefore less suitable to study the changes in phenol pattern in each separate leaf of a developing plant. In order to circumvent some of the above problems we developed a new method of phenol determination which is based on the in situ oxidation of these compounds, followed by difference spectrophotometry. Here, we demonstrate with Salvia occidentalis, an obligate SD' plant, a method whereby a dynamic picture of phenol metabolism as a function of environmental conditions like daylength, light intensity, night interruption, and temperature can be obtained. Possible implications of these data in the mechanism of flower induction will be discussed. MATERIALS AND METHODSPlant Material and Growing Techniques. S. occidentalis plants were propagated by cuttings from a parent plant from a clone previously used by Meijer (6) in our laboratory for his investiga-'Abbreviations: SD: short day; LD: long day.

Identification of Phenolic Compounds and Determination of Antioxidant Activity in Extracts and Infusions of Salvia Leaves

“…The harvest time also had an effect on its content. Engelsma [ 48 ] showed that in the light period the content of phenol (mainly chlorogenic and isochlorogenic acid) increases proportionally to the length of this period, which is also confirmed by our research.…”

Influence of Light Intensity and Photoperiod on the Growth and Phenol Contents of Hydroponic Basil in Vertical Farms

“…하지만 Zhang 등(2020)의 연구에 따 르면 광주기가 길어질수록 chlorophyll a, chlorophyll b, total chlorophyll의 함량이 감소하며 MDA의 축적이 증가한다고 보고되었는데 이는 본 실험과 유사할 것으로 예상된다. 페놀 은 식물의 항산화 물질 중 하나로 세포질 경계면에서 합성 하여 액포로 운반하는 과정을 거치는데 야간에는 이동이 멈추면서 분해되지만 주간에는 이동하여 함량이 증대된다(Engelsma, 1978;Engelsma, 1979; Kim 등, 2022). 광의 영 향으로 작물의 생합성 축적이 좌우되는데 광주기가 긴 것이 짧은 것보다 ROS 생성을 더 크게 유도하면서 페놀 화합물이 증가된다고 한다(de Andrade 등, 2021).…”