Effect of daidzein on cell growth, cell cycle, and telomerase activity of human cervical cancer in vitro

Guo, Junming; Kang, Ge-Fei; Xiao, Bingxiu; Liu, D. H.; Zhang, S.

doi:10.1111/j.1048-891x.2004.14525.x

Cited by 36 publications

(19 citation statements)

References 18 publications

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“…2C). Interestingly, flow cytometric analysis from other study [31] demonstrated human colorectal carcinoma SW620 cells were arrested at S and G2 /M phase with Harmine treatment. This indicates that different types of tumor cell lines respond differently to Harmine’s treatment, and multiple mechanisms might be responsible for cell cycle arrest in various cancer cells.…”

Section: Discussionmentioning

confidence: 98%

Suppression of Non-Small Cell Lung Cancer Growth and Metastasis by a Novel Small Molecular Activator of RECK

Shen¹,

Bang-hua²,

Zhang³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Reversion-inducing cysteine-rich protein with kazal motifs (RECK) is a novel tumor suppressor gene that is critical for regulating tumor cell invasion and metastasis. The expression of RECK is dramatically down-regulated in human cancers. Harmine, a tricyclic compound from Peganum harmala, has been shown to have potential anti-cancer activity. Methods: Cell proliferation assay (CCK-8 cell viability assay), cell cycle analysis (detection by flow cytometry), apoptosis staining assay (TUNEL staining), cell migration assay and invasion assay (transwell assay) were carried out to investigate the Harmine’s efficacy on non-small cell lung cancer (NSCLC) cells in vitro. A549-luciferase cell orthotropic transplantation xenograft mouse model was used to determine the effect of Harmine treatment on NSCLC in vivo. Western blotting analysis of cell growth and metastasis related signal pathways was conducted to investigate the molecular mechanism of Harmine’s inhibitory effect on NSCLC. Results: Harmine treatment effectively inhibited cell proliferation and induced the G1/S cell cycle arrest of NSCLC cells. Further study proved that Harmine treatment led to apoptosis induction. Furthermore, treatment with NSCLC cells with Hamine resulted in decreased cell migration and cell invasion in vitro. More importantly, Harmine treatment significantly suppressed the NSCLC tumor growth and metastasis in mouse xenograft model in vivo. Mechanistically, in Harmine-treated NSCLC cells, RECK expression and its downstream signaling cascade were dramatically activated. As a consequence, the expression level of MMP-9 and E-cadherin were significantly decreased. Conclusion: These findings identify Harmine as a promising activator of RECK signaling for metastatic NSCLC treatment.

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Section: Discussionmentioning

confidence: 98%

Suppression of Non-Small Cell Lung Cancer Growth and Metastasis by a Novel Small Molecular Activator of RECK

Shen¹,

Bang-hua²,

Zhang³

et al. 2018

Cell Physiol Biochem

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“…Although these bioactive compounds usually act as antioxidants, they are characterised by a dual activity, and may also behave as pro-oxidants and trigger carcinogenesis depending on their concentration, target molecule and specific environment. [18][19][20][21][22] The prevalence of both properties in a single compound can be beneficial in terms of rationale preventive or therapeutic purposes.…”

Section: A Introductionmentioning

confidence: 99%

Raman microspectroscopy for probing the impact of a dietary antioxidant on human breast cancer cells

et al. 2016

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Breast cancer is the second most common type of cancer worldwide and the most frequent among women, being the fifth cause of death from neoplastic disease. Since this is an oxidative-stress related neoplasia, it is largely preventable. A dietary isoflavone abundant in soybean -daidzein -was currently investigated as to its chemopreventive and/or chemotherapeutic properties towards the human MDA-MB-231 (metastatic, estrogen-unresponsive) and MCF-7 (estrogen-responsive) breast cancer cell lines. Biological assays for evaluation of antitumour and anti-invasive activities were combined with state-of-theart vibrational microspectroscopy techniques. At 50 and 100 µM concentrations and 48 h incubation time, daidzein was found to induce a marked decrease in cell viability (ca. 50%) for MDA-MB-231 and MCF-7 cells (respectively ca. 50% and 42%) and 40% inhibition of cell migration 48 h. MicroRaman analysis of fixed cells upon exposure to this isoflavone unveiled its metabolic impact on both cell lines. Multivariate data analysis (unsupervised PCA) led to a clear discrimination between control and DAID-exposed cells, with distinctive effects on their biochemical profile, particularly regarding DNA, lipids and protein components, in a cell-dependent way. This is the first reported study on the impact of dietary antioxidants on cancer cells by microRaman techniques. A IntroductionCancer is one of the major causes of morbidity and mortality worldwide, closely following cardiovascular disorders. Breast carcinoma, in particular, is an important public health problem since it is the second most lethal cancer among women worldwide: according to the World Health Organisation (WHO), it affects 28% of the women in Europe 1 , being the second most lethal cancer type in Portugal (16%). 2 Epidemiological evidence has demonstrated a clear difference in breast cancer incidence between eastern and western countries, asian women being the least affected, which can be closely related to dietary habits.Phytochemicals, secondary plant metabolites widespread in fruits, vegetables, cereals and beverages such as tea, coffee and wine, and very abundant in a mediterranean diet, are an abundant source of dietary antioxidants known to significantly decrease the harmful effect of oxidative species, thus being promising chemopreventive agents towards carcinogenesis. [3][4][5][6] Actually, several studies have established a direct relationship between the intake of polyphenols and the prevention of oxidative stress-induced diseases, namely neurodegenerative and cardiovascular disorders, and some types of cancer. 7,8 The isoflavone daidzein (DAID), in particular, is the second most abundant component of soybean (following genistein), a growingly consumed food product, which renders this isoflavone a very suitable target of study regarding its potential health beneficial effects. In addition, it exhibits structural and functional similarities to the endogenous hormone estrogen 9 (Fig. 1). Since some breast cancers are estrogen-dependent (ER + ), DAID may...

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“…The growth inhibition function of the two isoflavones on cancer cells vary with concentration, cell type and exposure time. For example, pure genistein and daidzein might exhibit biphasic effect on cancer cells, that is, the compounds showed inhibitory effect at high concentrations but displayed activating function at low concentrations [15][16][17]; or lower concentrations had more obvious apoptosis induction than higher concentrations [18]. It has also been reported that the two compounds alone inhibited certain malignant phenotype of melanoma via different mechanisms.…”

Section: Introductionmentioning

confidence: 99%

“…It has also been reported that the two compounds alone inhibited certain malignant phenotype of melanoma via different mechanisms. Pure genistein induced HeLa cell apoptosis through inhibition of Topo IIα expression [19], while daidzein induced HeLa cell apoptosis through inhibition of telomerase activity [18]. It is interesting that a genistein-rich extract containing daidzein is more effective in inhibiting cancer cell growth than purified genistein in the basis of same genistein concentration [20].…”

Section: Introductionmentioning

confidence: 99%

Soy-derived Isoflavones Inhibit HeLa Cell Growth by Inducing Apoptosis

Xiao

Huang

Geng

et al. 2011

Plant Foods Hum Nutr

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Isoflavones are among the major bioactive compounds found in a wide variety of plant-derived foods, especially in soybeans and soy-based foods. In this study, the effect of a soy-derived isoflavone mixture (designated as SI-I, containing 71% daidzein, 14.3% genistein and 14.7% glycitein) on HeLa cells and its mechanism were investigated. SI-I in concentration range 5-80 μg/ml significantly reduced the survival rate of HeLa cells by MTT assay, whereas showed no side effect on that of L929 cells. After HeLa cells were exposed to 10, 20 and 40 μg/ml SI-I for 4 days, typical apoptotic morphological changes, including nuclear fragmentation, cytoplasm shrinkage and decrease of cell volume, were observed by fluorescence microscope and CLSM, respectively. FCM analysis revealed that the percentages of early apoptotic cells with lost Δψm increased by 2.27, 2.74 and 4.05 folds respectively, compared with control. The results showed that SI-I inhibited HeLa cell growth through inducing apoptosis via the mitochondrial pathway and comparisons with reported data indicated that synergistic effect existed between the isoflavone species contained in SI-I. It is proposed that natural soy-derived isoflavones are potential candidates as chemotherapeutic agents against human cervical cancer.

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Effect of daidzein on cell growth, cell cycle, and telomerase activity of human cervical cancer in vitro

Cited by 36 publications

References 18 publications

Suppression of Non-Small Cell Lung Cancer Growth and Metastasis by a Novel Small Molecular Activator of RECK

Suppression of Non-Small Cell Lung Cancer Growth and Metastasis by a Novel Small Molecular Activator of RECK

Raman microspectroscopy for probing the impact of a dietary antioxidant on human breast cancer cells

Soy-derived Isoflavones Inhibit HeLa Cell Growth by Inducing Apoptosis

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