Effect of dacarbazine on CD44 in live melanoma cells as measured by atomic force microscopy-based nanoscopy

Huang, Xun; He, Jiexiang; Zhang, Huan-tian; Sun, Kai; Yang, Jie; Wang, Huajun; Guo, Zhenzhao; Zha, Zhengang; Zhou, Changren

doi:10.2147/ijn.s149107

Cited by 7 publications

(8 citation statements)

References 66 publications

(90 reference statements)

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“…The results demonstrated DTIC affects, but not completely inhibits, the binding of CD44 ligand to membrane receptors, suggesting the reason for the poor prognosis associated with DTIC treatment of melanoma. Therefore, AFM gives thermodynamic and kinetic insights into the effect of DTIC on the CD44 ligand-binding process (Hj et al, 2017). Bobrowska et al (2019) also demonstrated that vertical growth phase melanoma cells showed surface biochemical and biomechanical changes in parallel, which could be used as a fingerprint of melanoma progression (Bobrowska et al, 2019).…”

Section: Metastizationmentioning

confidence: 99%

Melanoma in the Eyes of Mechanobiology

Brás

Radmacher

Sousa

et al. 2020

Front. Cell Dev. Biol.

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Skin is the largest organ of the human body with several important functions that can be impaired by injury, genetic or chronic diseases. Among all skin diseases, melanoma is one of the most severe, which can lead to death, due to metastization. Mechanotransduction has a crucial role for motility, invasion, adhesion and metastization processes, since it deals with the response of cells to physical forces. Signaling pathways are important to understand how physical cues produced or mediated by the Extracellular Matrix (ECM), affect healthy and tumor cells. During these processes, several molecules in the nucleus and cytoplasm are activated. Melanocytes, keratinocytes, fibroblasts and the ECM, play a crucial role in melanoma formation. This manuscript will address the synergy among melanocytes, keratinocytes, fibroblasts cells and the ECM considering their mechanical contribution and relevance in this disease. Mechanical properties of melanoma cells can also be influenced by pigmentation, which can be associated with changes in stiffness. Mechanical changes can be related with the adhesion, migration, or invasiveness potential of melanoma cells promoting a high metastization capacity of this cancer. Mechanosensing, mechanotransduction, and mechanoresponse will be highlighted with respect to the motility, invasion, adhesion and metastization in melanoma cancer.

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Section: Metastizationmentioning

confidence: 99%

Melanoma in the Eyes of Mechanobiology

Brás

Radmacher

Sousa

et al. 2020

Front. Cell Dev. Biol.

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“…It has been shown that CD34 and CD44 play a key role in the regulation of several properties of CSCs, such as self-renewal, metastasis, tumor initiation, chemo-and radio-resistance, and colonization [18,19].For this reason, in the present study, the expression levels oftwo cell-surface markers, i.e.CD34 and CD44, were examined before and after treatment with metformin by ow cytometry.CD34 is a glycoprotein expressed on hematopoietic stem cells and capillary endothelial cells. CD34 has been found to be over expressedin aggressive tumor cells and this has been veri ed by molecular methods [12]. In this study, as suggested in some previous studies, we assumed that this molecule could be related to endothelial and stemness phenotype in tumor cells and facilitate cell migration [6,18].…”

Section: Resultsmentioning

confidence: 57%

“…Some studies have shownthat the CD44 ligand-receptor complexes on dacarbazine-treated melanoma cells are less stable than on untreated cells. However, the CD44 receptor could still be activated, which, in turn, could lead to the activation of intracellular signaling and, consequently, a cellular response [12].…”

Section: Discussionmentioning

confidence: 99%

“…Dacarbazine belongs to a group of drugs called alkylating agent [12].Metformin (1, 1-dimethylbiguanide hydrochloride) is used for diabetes mellitus type 2 rst line therapy. The primary effect of metformin is based on interfering with respiratory complex I, reducing ATP production and activation of adenosine monophosphate-activated kinase (AMPK) that can activate intracellular pathways [13].…”

Section: Introductionmentioning

confidence: 99%

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The Role of PI3K and Wntsignaling Pathways and CSC Markers in Melanoma (B16F10) Therapy

sajedianfard

et al. 2021

Preprint

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Background Metformin has been the subject of recent studies aimed at the treatment of melanoma cancer. In this study, the anti-cancer effects of metformin, an antidiabetic drug, was investigated in-vitrousing the B16F10 melanoma cell line. Methods Melanoma cells were treated for 24 h with various concentrations of metformin, alone or incombination withdacarbazine. The effects of these two treatment agents on cell viability were evaluated by MTT assay. In addition, stemness and the activation of specific signaling pathways were evaluated by FACS and immunoblotting. Results Metformin induced β-catenin phosphorylation and decreasedmTOR and PARP expressions. Also, a normal dose of metformin was found toreducethe phosphorylation levels of4E-BP1, AKT, and S6rp.In this study, we evaluated the potential of metformin as a therapeutic agent against CSCs in the adjuvant setting. Conclusion Our data indicate that some transcriptional regulators and proteins in the above-mentioned pathwayswere associated with cancer progression and inhibited by adjuvant chemotherapy with metformin.Metformin significantly inhibited cell growth and proliferation pathways, including Wnt and PI3K/AKT/mTOR. These findings show the potential of metformin in cancer treatment.

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“…In some cancer research, AFM can be used as a high-imaging technique to analyze morphological changes for the evaluation of drug effects. Further, mesenchymal stem cell shape is changed during chondrogenic induction [ 18 ]. While changes of cell shape appear to be necessary for differentiation, little is known about whether cell morphology affects earlier developmental stages of mesenchymal stem cell differentiation.…”

Section: Resultsmentioning

confidence: 99%

Atomic Force Microscopy-Based Nanoscopy of Chondrogenically Differentiating Human Adipose-Derived Stem Cells: Nanostructure and Integrin β1 Expression

Yang

Huang

et al. 2018

Nanoscale Res Lett

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Integrin β1 is known to be involved in differentiation, migration, proliferation, wound repair, tissue development, and organogenesis. In order to analyze the binding probability between integrin β1 ligand and cluster of differentiation 29 (CD29) receptors, atomic force microscopy (AFM) was used to detect native integrin β1-coupled receptors on the surface of human adipose-derived stem cells (hADSc). The binding probability of integrin β1 ligand–receptor interaction was probed by integrin β1-functionalized tips on hADSc during early chondrogenic differentiation at the two-dimensional cell culture level. Cell morphology and ultrastructure of hADSc were measured by AFM, which demonstrated that long spindled cells became polygonal cells with decreased length/width ratios and increased roughness during chondrogenic induction. The binding of integrin β1 ligand and CD29 receptors was detected by β1-functionalized tips for living hADSc. A total of 1200 curves were recorded at 0, 6, and 12 days of chondrogenic induction. Average rupture forces were, respectively, 61.8 ± 22.2 pN, 60 ± 20.2 pN, and 67.2 ± 22.0 pN. Rupture events were 19.58 ± 1.74%, 28.03 ± 2.05%, and 33.4 ± 1.89%, respectively, which demonstrated that binding probability was increased between integrin β1 ligand and receptors on the surface of hADSc during chondrogenic induction. Integrin β1 and the β-catenin/SOX signaling pathway were correlated during chondrogenic differentiation. The results of this investigation imply that AFM offers kinetic and visual insight into the changes in integrin β1 ligand–CD29 receptor binding on hADSc during chondrogenesis. Changes in cellular morphology, membrane ultrastructure, and the probability of ligand–transmembrane receptor binding were demonstrated to be useful markers for evaluation of the chondrogenic differentiation process.Electronic supplementary materialThe online version of this article (10.1186/s11671-018-2722-z) contains supplementary material, which is available to authorized users.

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Effect of dacarbazine on CD44 in live melanoma cells as measured by atomic force microscopy-based nanoscopy

Cited by 7 publications

References 66 publications

Melanoma in the Eyes of Mechanobiology

Melanoma in the Eyes of Mechanobiology

The Role of PI3K and Wntsignaling Pathways and CSC Markers in Melanoma (B16F10) Therapy

Atomic Force Microscopy-Based Nanoscopy of Chondrogenically Differentiating Human Adipose-Derived Stem Cells: Nanostructure and Integrin β1 Expression

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