Effect of cytokinins on tobacco mosaic virus production in tobacco pith tissue cultures

Milo, George E.; Srivastava, B. I. Sahai

doi:10.1016/0042-6822(69)90118-4

Cited by 14 publications

(3 citation statements)

References 7 publications

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“…It has been suggested that to achieve pathogenesis in the host, CK-secreting biotrophs or hemibiotrophs manipulate CK signaling to regulate the host cell cycle and nutrient allocation ( 13 ). Exogenous application of CK was reported to reduce plant infection by powdery mildew ( 10 ), smut fungi ( 14 ), and viruses ( 15 ). Conidia, mycelia, and germinating uredospores of Puccinia graminis and P. recondita have been shown to accumulate CK, manipulating CK signaling to regulate the host plant cell cycle ( 16 , 17 ).…”

Section: Introductionmentioning

confidence: 99%

Cytokinin Inhibits Fungal Development and Virulence by Targeting the Cytoskeleton and Cellular Trafficking

Gupta

Anand

Pizarro

et al. 2021

mBio

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Section: Introductionmentioning

confidence: 99%

Cytokinin Inhibits Fungal Development and Virulence by Targeting the Cytoskeleton and Cellular Trafficking

Gupta

Anand

Pizarro

et al. 2021

mBio

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“…Several workers showed that normal constituents of tissue culture media had significant effects on virus multiplication, either directly or indirectly by affecting the metabolism of the cultured cells (KUTSKY and RAWLINS 1950, KASSANIS 1957, MiLO and SRIVASTAVA 1969. However, the effects were often conflicting and the results were not always conclusive.…”

Section: Discussionmentioning

confidence: 99%

Resistance Mechanism of Cultured Plant Cells to Tobacco Mosaic Virus

Toyoda

Oishi

Matsuda

et al. 1985

Journal of Phytopathology

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In somaclonal tissues obtained from systemically TMV‐infected tobacco plants, a relation between changes of TMV amounts and the callus growth was examined. The culture medium was suitable for maintaining a constant concentration of TMV as well as active callus growth. By using the shake‐culture method, somaclonal tissues were separated into two classes on the basis of callus sizes. In large callus tissues, TMV amounts were constant during subculturing but the tissues did not either grow or release the newly divided cells after the last subculture. On the other hand, smaller callus tissues grew markedly and the TMV amounts were conspicuously lowered. After shake‐subculture of smaller tissues, they were successfully regenerated to plantlets. None of the plantlets expressed any mosaic symptoms, while plantlets from the original somaclones showed severe mosaic symptoms of TMV in leaflets. Thus, the present report describes the successful production of virus‐free plantlets from infected somaclonal callus cultures.

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“…It has been suggested that to achieve pathogenesis in the host, CK-secreting biotrophs or hemibiotrophs manipulate CK signaling to regulate the host cell cycle and nutrient allocation (13). Exogenous application of CK was reported to reduce plant infection of powdery mildew (10,14), smut fungi (15), and viruses (16). Conidia, mycelia of some fungi, and germinating uredospores of Puccinia graminis and P. recondite have been shown to accumulate CK, manipulating CK signaling to regulate host plant cell cycle (17,18).…”

Section: Introductionmentioning

confidence: 99%

Cytokinin inhibits fungal development and virulence by targeting the cytoskeleton and cellular trafficking

Gupta

Anand

Pizarro

et al. 2020

Preprint

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Cytokinin (CK) is an important plant developmental regulator, having activities in many aspects of plant life and its response to the environment. CKs are involved in diverse processes in the plant, including stem-cell maintenance, vascular differentiation, growth and branching of roots and shoots, leaf senescence, nutrient balance and stress tolerance. In some cases, phytopathogens secrete CKs. It has been suggested that to achieve pathogenesis in the host, CK-secreting biotrophs manipulate CK signaling to regulate the host cell cycle and nutrient allocation. CK is known to induce host plant resistance to several classes of phytopathogens from a handful of works, with induced host immunity via salicylic acid signaling suggested to be the prevalent mechanism for this host resistance. Here, we show that CK directly inhibits the growth, development, and virulence of fungal phytopathogens. Focusing on Botrytis cinerea (Bc), we demonstrate that various aspects of fungal development can be reversibly inhibited by physiological concentrations of CK. We also found that CK affects both budding and fission yeast in a similar manner. Investigating the mechanism by which CK influences fungal development, we found that CK affects cellular trafficking in Bc, lowering endocytic rates and endomembrane compartment sizes, likely leading to reduced growth rates and arrested developmental programs. Mutant analyses in yeast confirmed that the endocytic pathway is a CK target. Our work uncovers a remarkably conserved role for a plant growth hormone in fungal biology, suggesting that co-evolution between pathogen and host resulted in fascinating molecular adaptations on fundamental processes in eukaryotic biology.

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Effect of cytokinins on tobacco mosaic virus production in tobacco pith tissue cultures

Cited by 14 publications

References 7 publications

Cytokinin Inhibits Fungal Development and Virulence by Targeting the Cytoskeleton and Cellular Trafficking

Cytokinin Inhibits Fungal Development and Virulence by Targeting the Cytoskeleton and Cellular Trafficking

Resistance Mechanism of Cultured Plant Cells to Tobacco Mosaic Virus

Cytokinin inhibits fungal development and virulence by targeting the cytoskeleton and cellular trafficking

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