Effect of Cytokines and Chemokines on Sickle Neutrophil Adhesion to Fibronectin

Assis, Ângela Maria de; Conran, Nicola; Canalli, Andréia A.; Lorand-Metze, Irene; Saad, Sara T.O.; Costa, Fernando Ferreira

doi:10.1159/000083451

Cited by 55 publications

(61 citation statements)

References 46 publications

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“…It has been shown that the adhesive molecules involved in increased adhesiveness of granulocytes and monocytes and leukocytes interaction with endothelial cells are the cytokine-induced L-and P-selectin (CD-62L), β2-integrin CD11b/C18 (CD-11b) and LFA-1 (CD11a/CD18) [49][50][51][52]. L-selectin (CD62L) facilitates rolling and temporary arrest of circulating leukocytes on endothelium surface [53], whereas β2-integrin (CD11b) is involved in subsequent stable adhesion [54,55].…”

Section: Discussionmentioning

confidence: 99%

Omega 3 (n−3) fatty acids down-regulate nuclear factor-kappa B (NF-κB) gene and blood cell adhesion molecule expression in patients with homozygous sickle cell disease

Daak

Elderdery

Elbashir

et al. 2015

Blood Cells, Molecules, and Diseases

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Chronic inflammation and reduced blood levels of omega-3 fatty acids (n−3) are known characteristics of sickle cell disease (SCD).The anti-inflammatory properties of n−3 fatty acids are well recognized. Omega-3 treated (n = 24), hydroxyurea (HU) treated (n = 18), and n−3 untreated (n = 21) homozygous SCD patients (HbSS) and healthy (HbAA) controls (n = 25) matched for age (5-16 years), gender and socioeconomic status were studied. According to age (5-10) or (11-16) years, two or three capsules containing 277.8 mg docosahexaenoic (DHA) and 39.0 mg eicosapentaenoic (EPA) or high oleic acid placebo (41%) were assigned to n − 3 treated and n − 3 untreated groups, respectively. Hydroxyurea treated group was on dosage more than 20 mg/kg/day. The effect of supplementation on systemic and blood cell markers of inflammation was investigated. The n− 3 treated group had higher levels of DHA and EPA (p b 0.001) and lower white blood cell count and monocyte integrin (p b 0.05) compared with the n−3 untreated. No difference was detected between the two groups regarding C-reactive protein, granulocytes integrin and selectin, plasma tumour necrosis factor-α and interleukin-10. The n−3 treated group had lowered nuclear factor-kappa B (NF-κB) gene expression compared to n−3 untreated and HU treated groups (p b 0.05). This study provides evidence that supplementation with n− 3 fatty acids may ameliorate inflammation and blood cell adhesion in patients with SCD.

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Section: Discussionmentioning

confidence: 99%

Omega 3 (n−3) fatty acids down-regulate nuclear factor-kappa B (NF-κB) gene and blood cell adhesion molecule expression in patients with homozygous sickle cell disease

Daak

Elderdery

Elbashir

et al. 2015

Blood Cells, Molecules, and Diseases

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“…A ativação dos monócitos, com a secreção de citocinas pró-inflamatórias (IL-1 e TNF-α), leva os leucócitos, em número constantemente elevado, a aderirem ao endotélio inflamado e a interagirem com as hemácias falcizadas [44][45][46][47][48][49] .…”

Section: As Doenças Falciformesunclassified

The genetics of blood disorders: the hereditary hemoglobinopathies

Sonati¹,

Costa²

2008

J Pediatr (Rio J)

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ResumoObjetivo: Sumarizar os dados disponíveis na literatura recente sobre os aspectos fisiopatológicos, de diagnóstico e tratamento das doenças falciformes e da talassemia β, hemoglobinopatias hereditárias de maior relevância nas populações. Fontes dos dados AbstractObjective: To summarize recently published data on the pathophysiology, diagnosis and treatment of sickle cell diseases and β-Thalassemias, the most relevant hereditary hemoglobinopathies in the global population.Sources: Searches were run on the MEDLINE and SCIELO databases, limited to the period from 2003 to May 2008, using the terms hereditary hemoglobinopathies, sickle cell diseases and β-thalassemia. Two books and two chapters were also included. Summary of the findings:More than 2,000 articles were identified; those providing the most important information and broadest views were selected.Conclusions: Morbidity and mortality rates from sickle cell diseases and β-thalassemia are still very high and represent an important challenge. Increased understanding of pathophysiological aspects has lead to significant improvements in treatment and prevention of these diseases.

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“…The recruitment of large, less deformable, adherent white cells to the vascular endothelium and their interaction with circulating erythrocytes may impair blood flow. SCA neutrophils differ from those of healthy individuals, as exemplified by their enhanced adhesion to fibronectin and increased spontaneous and chemokine-stimulated chemotaxis in vitro (Assis et al, 2005;Canalli et al, 2007). In SCA, neutrophils overexpress a variety of adhesion molecules on their surface that are involved in cell adhesion and transendothelial migration (Canalli et al, 2011).…”

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confidence: 99%

Hydroxycarbamide reduces eosinophil adhesion and degranulation in sickle cell anaemia patients

et al. 2013

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SummaryInflammation, leucocyte and red cell adhesion to the endothelium contribute to the pathogenesis of sickle cell anaemia. Neutrophils appear to be important for vaso-occlusion, however, eosinophils may also participate in this phenomenon. The role of eosinophils in the pathophysiology of sickle cell anaemia (SCA) and the effect of hydroxycarbamide (HC) therapy on the functional properties of these cells are not understood. Patients with SCA and those on HC therapy (SCAHC) were included in the study. SCAHC individuals presented significantly lower absolute numbers of eosinophils than SCA. Furthermore, SCAHC eosinophils demonstrated significantly lower adhesive properties, compared to SCA eosinophils. SCA and SCAHC eosinophils presented greater spontaneous migration when compared with control eosinophils. Baseline eosinophil peroxidase and reactive oxygen species release was higher for SCA individuals than for control individuals, as were plasma levels of eosinophil derived neurotoxin. SCAHC eosinophil degranulation was lower than that of SCA eosinophil degranulation. Eotaxin-1 and RANTES levels were higher in the plasma of SCA and SCAHC individuals, when compared with controls. These data suggest that eosinophils exist in an activated state in SCA and indicate that these cells play a role in the vaso-occlusive process. The exact mechanism by which HC may alter SCA eosinophil properties is not clear.

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Effect of Cytokines and Chemokines on Sickle Neutrophil Adhesion to Fibronectin

Cited by 55 publications

References 46 publications

Omega 3 (n−3) fatty acids down-regulate nuclear factor-kappa B (NF-κB) gene and blood cell adhesion molecule expression in patients with homozygous sickle cell disease

Omega 3 (n−3) fatty acids down-regulate nuclear factor-kappa B (NF-κB) gene and blood cell adhesion molecule expression in patients with homozygous sickle cell disease

The genetics of blood disorders: the hereditary hemoglobinopathies

Hydroxycarbamide reduces eosinophil adhesion and degranulation in sickle cell anaemia patients

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