Effect of Cysteamine on the Freezing of Buck Semen

Ertürk, Ezgi; Şenlikçi, Hatice; Sandal, Asiye İ̇zem; Özdaş, Özen Banu; Ak, Kemal

doi:10.5152/actavet.2020.19029

Cited by 1 publication

(3 citation statements)

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“…Several studies have suggested that the removal of buck SP prevents agglutination and positively affects sperm properties in frozen‐thawed semen (Cabrera et al., 2005). On the other hand, there are also studies reporting that the presence of SP does not have negative effects even though buck spermatozoa are agglutinated during freezing/thawing, and post‐thaw motility rates are found to be similar (Erturk et al., 2020; Tabarez et al., 2017). In the current study, there was no statistical difference between the groups, supporting the notion that the removal of SP did not have any role in the negative results encountered in the freezing of buck semen.…”

Section: Discussionmentioning

confidence: 99%

“…Buck spermatozoa coagulate in egg yolk-based diluting and freezing media, which are commonly preferred in several species, due to EYCE found in seminal plasma, causing a failure to achieve desired sperm properties after freezing and thawing procedures (Üstüner & Ülgen, 2009). Several studies have suggested that the removal of buck SP prevents agglutination and positively affects sperm properties in frozenthawed semen (Cabrera et al, 2005 also studies reporting that the presence of SP does not have negative effects even though buck spermatozoa are agglutinated during freezing/thawing, and post-thaw motility rates are found to be similar (Erturk et al, 2020;Tabarez et al, 2017). In the current study, there was no statistical difference between the groups, supporting the notion that the removal of SP did not have any role in the negative results encountered in the freezing of buck semen.…”

Section: Discussionmentioning

confidence: 99%

“…Harmful interaction between the egg yolk coagulating enzyme (EYCE) secreted by the bulbourethral gland, which makes up buck semen, with egg yolk is not present in cattle and ram (Sandal et al., 2020). Researchers tried to resolve this problem by separating the semen from its SP before freezing and reported that semen motility continued in diluents containing egg yolk when SP was separated and the semen coagulated and viability was affected when SP was re‐added (Erturk et al., 2020; Roy, 1957). The EYCE, also known as phospholipase A, hydrolysis egg yolk lecithin into fatty acids and lysolecithins, thus leading to a decrease in sperm motility and sperm viability while increasing the incidence of acrosomal damage for several species (Aboagla & Terada, 2004; Salamon & Ritar, 1982).…”

Section: Introductionmentioning

confidence: 99%

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Determination of oxidative stress parameters and DNA fragmentation on post‐thawed buck semen in the presence of ram seminal plasma and fetal calf serum

et al. 2021

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The aim of the study was to investigate the efficiency of ram seminal plasma and fetal calf serum on freezing of buck semen. Twenty ejaculates were collected using an electro‐ejaculator and split into six groups. While FCS additive was not used in A1, A2 and A3 groups, 10% FCS was added to B1, B2 and B3 groups. These groups were then edited according to whether the buck or ram SP was involved. The design of the groups was done as follows: Group A1 (control 1), group A2 without buck SP, group A3 containing ram SP instead of buck SP. Groups B1 (control 2), B2 and B3 were the FCS added forms of these groups. Progressive sperm motility percentages in Group A1 and Group B2 were found to be higher when compared to the lowest Group B3. There were no significant differences between the groups in neither the levels of reactive oxygen species nor the enzyme and glutathione activities. In conclusion, the lack of statistical difference between the groups suggested that despite the supplements used but only when the buck spermatozoa structure was healthy, the cell could preserve acrosome, DNA and the integrity of membrane.

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Section: Discussionmentioning

confidence: 99%