Effect of Cutting Size on the Performance of Exogenous Auxin IBA in Vegetative Propagation of Rubus erythroclados Mart. ex Hook. f.

Abstract

Aim: The climbing shrub Rubus erythroclados Mart. ex Hook. f. (amora-verde) has great food (fruits) and medicinal (leaves) potential. However, this species remains understudied. The objective of this study was to evaluate the interaction between cutting size and IBA concentration in the vegetative propagation of R. erythroclados by stem cuttings.
 Study Design: The experimental design used was completely randomized in a 3x5 factorial combination. The cutting size factor was represented by three levels (10, 15 and 20 cm) and IBA concentration factor by five levels (0, 1,000, 2,000, 3,000 and 4,000 mg L-1).
 Results: There was an interaction between the factors in 13 of the 19 variables analyzed, being: live cuttings at 30 and 60 days, live cuttings with callus formation, sprouted cuttings at 30, 60 and 90 days, rooted cuttings, shoot number, shoot diameter, root number, root length, length of the largest root and root diameter. Size increase of the R. erythroclados cuttings caused a decrease in the phytotoxic effect of IBA in the development of the aerial part. The effect of IBA in response to rooting induction, in the larger size of R. erythroclados cuttings, required an increase in IBA concentration compared to the cuttings of shorter size.
 Conclusion: Cuttings of 10 and 15 cm treated with 1,000 mg L-1 of IBA showed greater rooting (20.0%) compared to the other treatments, being, therefore, indicated for the vegetative propagation of R. erythroclados by stem cuttings.

Rooting of blackberry (Rubus fruticosus L.) hardwood cuttings as influenced by cutting time and cutting length

“…This could be related to the mobilization of more sugars and nutrients at the base of cuttings, during adventitious root generation (5). This finding is the same as found by Balestrin et al, (5).…”

“…This can also emphasize that, to some extent, the longer the cuttings, the longer the shoots emerged ( 14). This result is consistent with many previous studies' results, i. e.; Balestrin et al, (5) showed that 20-cm cuttings of Rubus erythroclados gave 52.7 cm shoot length which was significantly different compared to 10 and 15cm cuttings giving 18.7 and 41.8 cm shoot lengths, respectively. Kahramanoğlu and Umar (17) also indicated that the length of shoots that emerged from pomegranate cuttings is proportional to cutting length.…”

“…Twenty-centimeter cuttings again caused a significantly higher value (96.87%) of rooting percentage of blackberry cuttings compared to ten-centimeter cuttings which gave only 63.89% rooted cuttings. There are no studies in the literature involving Rubus species evaluating the effect of different sizes and lengths of cutting on rooting (5). These results are in consistent with the results of other studies conducted in other genera, (34) noticed that two-node non-leafy cutting of blackberries with more stored carbohydrates performed better-rooting results than one-node cuttings.…”

“…In this aspect, very scarce scientific works with blackberries have been done. (37) investigated the effect of cutting length (5,10,15, and 20 cm) in black raspberries, and they found that cutting lengths between10-20cm obtained better results for rooting cuttings and subsequent growth parameters.…”