Effect of Curcumin on miRNA Expression in Human Y79 Retinoblastoma Cells

Sreenivasan, Seethalakshmi; Thirumalai, Karthiyaini; Danda, Ravikanth; Krishnakumar, Subramanian

doi:10.3109/02713683.2011.647224

Cited by 64 publications

(39 citation statements)

References 32 publications

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“…Interestingly, emerging studies have demonstrated that some herbal product (e.g curcumin, camptothecin, andrographolide and resveratrol etc.) can perform their anti-tumor effects through affecting miRNAs expression [16, 23-25]. However, whether the antitumor effect of baicalein is related to its modulation of miRNA expression in HCC is still unknown.…”

Section: Discussionmentioning

confidence: 99%

“…In recent years, emerging studies have demonstrated that Chinese medicinal herbs can exert their anti-tumor effects through targeting miRNAs [15]. For example, curcumin could alter the miRNA expression profile in human retinoblastoma and pancreatic cancer cells, involving some well-known cancer-related miRNAs, such as miR-22, a tumor-suppressor miRNA was upregulated after curcumin treatment [16, 17]. Curcumin also can promote the apoptosis of cancer cells via miR-186, miR-15a and miR-16 and can inhibit the proliferation, invasion and metastasis of cells via miR-21, miR-208 [18-21].…”

Section: Introductionmentioning

confidence: 99%

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Baicalein, a Natural Anti-Cancer Compound, Alters MicroRNA Expression Profiles in Bel-7402 Human Hepatocellular Carcinoma Cells

Bie

Sun

et al. 2017

Cell Physiol Biochem

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Background/Aims: Baicalein has been shown to possess significant anti-hepatoma activity by inhibiting cell proliferation. Whether the anti-proliferative effect of baicalein is related to its modulation of miRNA expression in hepatocellular carcinoma (HCC) is still unknown. Methods: The anti-proliferative effects of baicalein on HCC cell line Bel-7402 was assessed by detecting the proliferation activity, cell cycle distribution, expression changes of p21/CDKN1A, P27/CDKN1B, total Akt and phosphoryted AKT. Microarray analysis was conducted to determine the miRNA expression profiles in baicalein-treated or untreated Bel-7402 cells and then validated by qRT-PCR in two HCC cell lines (Bel-7402 and Hep3B). The gain-of-function of miR-3127-5p was performed by detecting anti-proliferative effects after transfecting miRNA mimics in cells. Finally, the expression level of miR-3127-5p in different HCC cell lines was determined by qRT-PCR. Results: Baicalein was able to inhibit the proliferation of Bel-7402 cells by inducing cell cycle arrest at the S and G2/M phase via up-regulating the expression of p21/CDKN1A and P27/CDKN1B and suppressing the PI3K/Akt pathway. Baicalein could alter the miRNA expression profiles in Bel-7402 cells. Putative target genes for differentially expressed miRNAs could be enriched in terms of cell proliferation regulation, cell cycle arrest and were mainly involved in MAPK, PI3K-Akt, Wnt, Hippo and mTOR signaling pathways. MiR- 3127-5p, one of up-regulated miRNAs, exhibits low expression level in several HCC cell lines and its overexpression could inhibit cell growth of Bel-7402 and Hep3B cell lines by inducing S phase arrest by up-regulating the expression of p21and P27 and repressing the PI3K/Akt pathway. Conclusions: Modulation of miRNA expression may be an important mechanism underlying the anti-hepatoma effects of baicalein.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Baicalein, a Natural Anti-Cancer Compound, Alters MicroRNA Expression Profiles in Bel-7402 Human Hepatocellular Carcinoma Cells

Bie

Sun

et al. 2017

Cell Physiol Biochem

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“…Among the upregulated miRNAs is miR-22, and its overexpression inhibited cell proliferation and migration. The erythoblastic leukemia viral oncogene homolog 3 (Erbb3) was confirmed as its target in the same study [45]. …”

Section: Curcuminmentioning

confidence: 99%

Regulation of MicroRNAs by Natural Agents: New Strategies in Cancer Therapies

Phuah

Siddik

2014

BioMed Research International

108

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MicroRNAs (miRNAs) are short noncoding RNA which regulate gene expression by messenger RNA (mRNA) degradation or translation repression. The plethora of published reports in recent years demonstrated that they play fundamental roles in many biological processes, such as carcinogenesis, angiogenesis, programmed cell death, cell proliferation, invasion, migration, and differentiation by acting as tumour suppressor or oncogene, and aberrations in their expressions have been linked to onset and progression of various cancers. Furthermore, each miRNA is capable of regulating the expression of many genes, allowing them to simultaneously regulate multiple cellular signalling pathways. Hence, miRNAs have the potential to be used as biomarkers for cancer diagnosis and prognosis as well as therapeutic targets. Recent studies have shown that natural agents such as curcumin, resveratrol, genistein, epigallocatechin-3-gallate, indole-3-carbinol, and 3,3′-diindolylmethane exert their antiproliferative and/or proapoptotic effects through the regulation of one or more miRNAs. Therefore, this review will look at the regulation of miRNAs by natural agents as a means to potentially enhance the efficacy of conventional chemotherapy through combinatorial therapies. It is hoped that this would provide new strategies in cancer therapies to improve overall response and survival outcome in cancer patients.

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“…Curcumin exerted its inhibitory effect on nasopharyngeal carcinoma (NPC) metastasis through inhibiting expression of miR-125a-5p and subsequently increasing expression of P53. 50 The expression of p53 was associated with cancer aggressiveness correlating with increased grading of invasive cancer front and cancer depth. 51 Bcl-2 was originally identified as a key regulator of apoptosis, but there are increasing evidences that show that Bcl-2 could also promote cancer cell migration and invasion.…”

Section: Curcumin Modulates Mirnas To Restrain Cancer Invasion and Mementioning

confidence: 99%

Curcumin inhibits cancer progression through regulating expression of microRNAs

et al. 2017

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Curcumin, a major yellow pigment and spice in turmeric and curry, is a powerful anti-cancer agent. The anti-tumor activities of curcumin include inhibition of tumor proliferation, angiogenesis, invasion and metastasis, induction of tumor apoptosis, increase of chemotherapy sensitivity, and regulation of cell cycle and cancer stem cell, indicating that curcumin maybe a strong therapeutic potential through modulating various cancer progression. It has been reported that microRNAs as small noncoding RNA molecules are related to cancer progression, which can be regulated by curcumin. Dysregulated microRNAs play vital roles in tumor biology via regulating expressions of target genes and then influencing multiple cancer-related signaling pathways. In this review, we focused on the inhibition effect of curcumin on various cancer progression by regulating expression of multiple microRNAs. Curcumin-induced dysregulation of microRNAs may activate or inactivate a set of signaling pathways, such as Akt, Bcl-2, PTEN, p53, Notch, and Erbb signaling pathways. A better understanding of the relation between curcumin and microRNAs may provide a potential therapeutic target for various cancers.

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Effect of Curcumin on miRNA Expression in Human Y79 Retinoblastoma Cells

Abstract: These observations suggest that curcumin modulate the miRNA expression profile, thereby exerting its anticancer effects on RB cells.

Cited by 64 publications

References 32 publications

Baicalein, a Natural Anti-Cancer Compound, Alters MicroRNA Expression Profiles in Bel-7402 Human Hepatocellular Carcinoma Cells

Baicalein, a Natural Anti-Cancer Compound, Alters MicroRNA Expression Profiles in Bel-7402 Human Hepatocellular Carcinoma Cells

Regulation of MicroRNAs by Natural Agents: New Strategies in Cancer Therapies

Curcumin inhibits cancer progression through regulating expression of microRNAs

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