Effect of curcumin and pirfenidone on toxicokinetics of paraquat in rat by UPLC–MS/MS

Wang, Shuanghu; Zou, Lin; Su, Ke; Zhang, Jing; Zhang, Lijing; Gao, Zhimou; Wang, Zhiyi; Ma, Jianshe; Wang, Xianqin

doi:10.1556/1326.2017.00175

Cited by 20 publications

(4 citation statements)

References 21 publications

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“…Therefore, the acetonitrile proteinprecipitation method was chosen as a pretreatment method for samples. The optimization of the extract of target resulted in an acceptable sensitivity and matrix effect [24][25][26][27][28]. As for the complication of the rat plasma samples, the addition of 200 μL acetonitrile could meet the requirements of removing the proteins and other potential interferences out of 50 μL plasma samples.…”

Section: Resultsmentioning

confidence: 99%

Pharmacokinetics of isocorynoxeine in rat plasma after intraperitoneal administration by UPLC–MS/MS

Zhan

Wei

Ren

et al. 2020

Acta Chromatographica

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Isocorynoxeine is one of the main alkaloids in Chinese medicinal herbs, and has pharmacological activities such as antihypertensive, sedative, anticonvulsant, and neuronal protection. It is an effective component of Uncaria for the treatment of hypertension. In this study, we used a fast and sensitive ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) to detect isocorynoxeine in rat plasma and investigated its pharmacokinetics in rats. Six rats were given isocorynoxeine (15 mg/kg) by intraperitoneal (i.p.) administration. Blood (100 μL) was withdrawn from the caudal vein at 5 and 30 min and 1, 2, 4, 6, 8, 12, and 24 h after administration. Chromatographic separation was achieved using a UPLC BEH C18 column using a mobile phase of acetonitrile–0.1% formic acid with gradient elution. Electrospray ionization (ESI) tandem mass spectrometry in the multiple reaction monitoring (MRM) mode with positive ionization was applied. Intra-day and inter-day precisions (relative standard deviation, %RSD) of isocorynoxeine in rat plasma were lower than 12%. The method was successfully applied in the pharmacokinetics of isocorynoxeine in rats after intraperitoneal administration. The t1/2 of isocorynoxeine is 4.9 ± 2.1 h, which indicates quick elimination.

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Section: Resultsmentioning

confidence: 99%

Pharmacokinetics of isocorynoxeine in rat plasma after intraperitoneal administration by UPLC–MS/MS

Zhan

Wei

Ren

et al. 2020

Acta Chromatographica

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“…ESI positive and negative selection is often evaluated in methodological studies (Wang et al, ; Wei, Ye, Jiang, Zhang, & Wang, ; Wu et al, ; Zhang, Wen, Xiang, Ma, & Wang, ; Zhou et al, ). Gelsenicine is an alkaline compound, more suitable for ESI‐positive detection.…”

Section: Resultsmentioning

confidence: 99%

Pharmacokinetics and bioavailability of gelsenicine in mice by UPLC–MS/MS

Jin

et al. 2018

Biomedical Chromatography

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Gelsenicine is an indole alkaloid isolated from Gelsemium elegans Benth. In recent years, the role of G. elegans Benth preparations in anti-tumor, analgesic, dilatation and dermatological treatment has attracted attention, and it has been applied clinically, but it is easy to cause poisoning with its use. An UPLC-MS/MS method was established to determine the gelsenicine in mouse blood, and the pharmacokinetics of gelsenicine after intravenous (0.1 mg/kg) and intragastric (0.5 and 1 mg/kg) administration was studied. Deltalin was used as internal standard; a UPLC BEH C 18 column was used for chromatographic separation. The mobile phase consisted of acetonitrile and 10 mmol/L ammonium acetate (0.1% formic acid) with a gradient elution flow rate of 0.4 mL/min. Multiple reaction monitoring mode was used for quantitative analysis of gelsenicine in electrospray ionization positive interface. Proteins from mouse blood were removed by acetonitrile precipitation. A validation of this method was performed in accordance with the US Food and Drug Administration guidelines. In the concentration range of 0.05-100 ng/mL, the gelsenicine in the mouse blood was linear (r > 0.995), and the lower limit of quantification was 0.05 ng/mL. In the mouse blood, the intra-day precision RSD was <12%, the inter-day precision RSD was <15%, the accuracy ranged from 89.8 to 112.3%, the average recovery was >76.8%, and the matrix effect was between 103.7 and 108.4%, which meet the pharmacokinetic research requirements of gelsenicine. The UPLC-MS/MS method is sensitive, rapid and selective, and has been successfully applied to the pharmacokinetic study of gelsenicine in mice. The absolute bioavailability of gelsenicine is 1.13%.

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“…Incubation system was 190 μl and contained 100 mM of potassium phosphate buffer (pH 7.4), 1 mg/ml rat liver microsomes (RLMs, produced in the laboratory of Clinical Pharmacy of the Sixth Affiliated Hospital of Wenzhou Medical University 20,27–29 ), and different concentrations of naringenin and rivaroxaban. The reaction was initiated after the 5 min pre‐incubation at 37℃ in a shaking water bath and then launched by adding 10 μl NADPH with the final concentration of 1 mM.…”

Section: Methodsmentioning

confidence: 99%

Naringenin has an inhibitory effect on rivaroxaban in rats both in vitro and in vivo

Shi

Zhao

Chen

et al. 2021

Pharmacology Res & Perspec

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Food–drug interactions are reported to have some impacts on the pharmacokinetics and pharmacodynamics of various oral drugs. To better understand the effects of naringenin, one natural product in many fruits, on the pharmacokinetics of rivaroxaban, drug–drug interactions (DDIs) between naringenin and rivaroxaban in vitro were investigated in Sprague–Dawley (SD) rat liver microsomes. For the DDIs in vivo, 12 male SD rats were randomly divided into the experimental group and the control group with six rats in each group. Rats in the experimental group were pre‐treated with naringenin (10 mg/kg/day) for 2 weeks before the administration of rivaroxaban (10 mg/kg) by oral gavage, while the rats in the control group were given rivaroxaban (10 mg/kg) only once. The plasma concentration of rivaroxaban in rats was then measured by UPLC‐MS/MS. In vitro data indicated that naringenin could decrease the metabolic clearance rate of rivaroxaban with the IC 50 value of 38.89 μM, and exhibited a mixed inhibition to rivaroxaban (Ki =54.91 μM, aKi =73.33 μM, a = 0.74). In vivo data in rats revealed that as compared with that of the control group, the AUC (0– t ) value of rats in the experimental group was increased from 2406.28 ± 519.69 μg/h/L to 4005.04 ± 1172.76 μg/h/L, the C max value was increased from 310.23 ± 85.76 μg/L to 508.71 ± 152.48 μg/L, and the V z / F and CL z / F were decreased from 23.03 ± 4.81 L/kg to 16.2 ± 8.42 L/kg, 4.26 ± 0.91 L/h/kg to 2.57 ± 0.73 L/h/kg, respectively. These data indicated that naringenin had an inhibitory effect on the pharmacokinetics of rivaroxaban in rats, suggesting that the DDIs between naringenin and rivaroxaban might occur when they were co‐administered in the clinic.

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Effect of curcumin and pirfenidone on toxicokinetics of paraquat in rat by UPLC–MS/MS

Cited by 20 publications

References 21 publications

Pharmacokinetics of isocorynoxeine in rat plasma after intraperitoneal administration by UPLC–MS/MS

Pharmacokinetics of isocorynoxeine in rat plasma after intraperitoneal administration by UPLC–MS/MS

Pharmacokinetics and bioavailability of gelsenicine in mice by UPLC–MS/MS

Naringenin has an inhibitory effect on rivaroxaban in rats both in vitro and in vivo

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