Effect of cumulus cell coculture and oxygen tension on the in vitro developmental competence of bovine zygotes cultured singly

Goovaerts, Inge; Leroy, Jo; Soom, Ann Van; Clercq, J. B. P. De; Andries, S.; Bols, P.E.J.

doi:10.1016/j.theriogenology.2008.09.038

Cited by 53 publications

(54 citation statements)

References 36 publications

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“…In bovine, in disagreement with our results, some authors observed that blastocysts obtained after culture individually contained a lower number of cells than embryos cultured in large groups (O'Doherty et al, 1997;Larson and Kubisch, 1999). However, other authors did not find a significant decrease on blastocyst cell number of the embryos cultured singly (Goovaerts et al, 2009 and or in reduced groups (Hoelker et al, 2009). However, in the experiments of Goovaerts et al (2009 and, embryos were cultured with a cumulus cell coculture.…”

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confidence: 86%

“…However, other authors did not find a significant decrease on blastocyst cell number of the embryos cultured singly (Goovaerts et al, 2009 and or in reduced groups (Hoelker et al, 2009). However, in the experiments of Goovaerts et al (2009 and, embryos were cultured with a cumulus cell coculture.Apoptotic rates obtained in this work for IVEP blastocysts were similar to those obtained by other authors (,7% to et al, 2011). We observed that the in vitro culture of a reduced number of embryos impaired blastocyst production and also quality, in particular by increasing the apoptotic rate per blastocyst.…”

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Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Ahumada

Salvador

Cebrián-Serrano

et al. 2013

Animal

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When embryos are cultured individually or in small groups, blastocyst yield efficiency and quality are usually reduced. The aim of this work was to investigate the effect of supplementation of the embryo culture medium (CM) with several growth factors (GFs) on embryo development and apoptosis rate when a reduced number of embryos were in vitro cultured. Two experimental studies (ES) were carried out. In ES 1, five treatments were tested to study the effect of GF on embryo development: Control (,30 to 50 embryos cultured in 500 ml of CM); Control 5 (Five embryos cultured in 50 ml microdrops of CM), without addition of GF in either of the two control groups; epidermal GF (EGF); IGF-I; and transforming GF-a (TGF-a) (Five embryos were cultured in 50 ml microdrops of CM with 10 ng/ml EGF, 10 ng/ml IGF-I or 10 ng/ml TGF-a, respectively). In ES 2, following the results obtained in ES 1, four different treatments were tested to study their effect on embryo development and quality (number of cells per blastocyst and apoptotic rate): Control; Control 5; EGF, all three similar to ES 1; EGF 1 IGF-I group (five embryos cultured in 50 ml microdrops of CM with 10 ng/ml EGF and 10 ng/ml IGF-I). In both ESs, it was observed that a higher proportion of embryos cultured in larger groups achieved blastocyst stage than embryos cultured in reduced groups (22.6% v. 14.0%, 12.6% and 5.3% for Control v. Control 5, IGF-I, TGF-a groups in ES 1, and 24.9% v. 17.1% and 19.0% for Control v. Control 5 and EGF in ES 2, respectively; P , 0.05), with the exception of embryos cultured in medium supplemented with EGF (18.5%) or with EGF 1 IGF-I (23.5%), in ES 1 and ES 2, respectively. With regard to blastocyst quality, embryos cultured in reduced groups and supplemented with EGF, alone or combined with IGF-I, presented lower apoptosis rates than embryos cultured in reduced groups without GF supplementation (11.6% and 10.5% v. 21.9% for EGF, EGF 1 IGF-I and Control 5 groups, respectively; P , 0.05). The experimental group did not affect the total number of cells per blastocyst. In conclusion, this study showed that supplementation of the CM with EGF and IGF could partially avoid the deleterious effect of in vitro culture of small groups of bovine embryos, increasing blastocyst rates and decreasing apoptosis rates of these blastocysts.Keywords: bovine embryo, reduced groups, in vitro culture, growth factor, apoptosis ImplicationsIn bovine, in vitro embryo production (IVEP) is especially interesting when applying the ovum pick-up technique (OPU). However, a small number of oocytes per female are usually recovered after OPU, and when embryos are cultured individually or in a reduced group blastocyst yield efficiency and quality are generally reduced. The improvement of IVEP efficiency and quality with low numbers of embryos would be of great interest for applying this technique in the dairy industry. IntroductionIn vitro embryo production (IVEP) has numerous applications in various scientific areas such as animal production or biomedicine. IVEP-rela...

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Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Ahumada

Salvador

Cebrián-Serrano

et al. 2013

Animal

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“…The cumulus cells (CC) monolayer was prepared using 55 in vitro matured COCs, as previously described (Goovaerts et al, 2009). Cumulus cells were mechanically removed by multiple pipetting, using a stripping pipette (Cook Medical, Bloomington, IN, USA).…”

Section: Preparation Of Cumulus Cells Monolayer (Cc Monolayer)mentioning

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Urokinase-type plasminogen activator does not affect in vitro bovine embryo development and quality

Krania

Dovolou

Rekkas

et al. 2015

Acta Veterinaria Hungarica

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The effects of modification of the in vitro embryo culture media (IVC) with the addition of urokinase-type plasminogen activator (u-PA) on the yield and/or quality of bovine embryos were examined in two experiments. In Experiment 1, denuded embryos were cultured in semi-defined synthetic oviductal fluid (SOF) for seven days, while in Experiment 2 embryos were co-cultured with cumulus cell monolayer in a serum-containing SOF medium. Plasminogen activator activity (PAA) and plasminogen activator inhibition (PAI) were determined in all spent IVC media. At the activity used (5 IU/ml), u-PA had no effect either on in vitro embryo production rates or on embryo quality as revealed by gene expression analysis of 10 important mRNA transcripts related to apoptosis, oxidation, implantation and metabolism. PAA and PAI analysis indicated the need for wellbalanced plasminogen activators and inhibitors as a culture environment for embryo development. However, more research is needed to unveil the mechanism by which u-PA is involved in in vitro embryo production systems.

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“…Estos resultados son diferentes a los reportados por Goovaerts et al (2009) quienes observaron que el cocultivo era beneficioso para la producción de blastocistos provenientes de ovocitos no vitrificados, obteniendo un aumento en la producción de blastocistos de 2,9 a 21,8% (p<0,05).…”

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Producción de embriones bovinos (Bos taurus) in vitro en co-cultivo a partir de ovocitos vitrificados en estadío maduro

Mendoza¹,

Colca²

2016

Rev. cient. UNTRM, Cienc. nat. ing.

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Producción de embriones bovinos (Bos taurus) in vitro en cocultivo a partir de ovocitos vitrificados en estadío maduroIn vitro embryo production (Bos taurus) in co-culture system from vitrified mature oocytes 1 2Yander Mavila Briceño Mendoza , Ilse Cayo Colca RESUMENEl objetivo de este trabajo fue estudiar el efecto del co-cultivo en la producción de embriones in vitro a partir de ovocitos vitrificados en estadío maduro. Los ovocitos fueron aspirados de ovarios colectados del Camal Municipal de la ciudad de Chachapoyas, luego vitrificados en estadío maduro in vitro (MIV). Posteriormente a su vitrificación y descongelamiento, se fertilizó con espermatozoides seleccionados y capacitados, finalmente se realizó el co-cultivo. La producción de blastocistos del grupo control (óvulos sin vitrificar), sin co-cultivo y con co-cultivo (células del cumulus) fue de 24,9, 9,9 y 9,6%, respectivamente. Los estudios continúan realizándose.Palabras claves: blastocitos, células del cumulus, co-cultivo, ovocitos, vitrificación. ABSTRACTThe aim of this investigation was to study the effect of co-culture system on the in vitro production of embryos from vitrified matured oocytes. The oocytes were aspirated from ovaries collected from the local slaughterhouse from the city of Chachapoyas, then they were matured in vitro. Next, they were vitrified and fertilized in vitro with selected sperm. Finally they were cultured in a co-culture system. The production of blastocysts was 24.9, 9.9 y 9.6%, in control, culture system and without co-culture system, respectively.

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Effect of cumulus cell coculture and oxygen tension on the in vitro developmental competence of bovine zygotes cultured singly

Cited by 53 publications

References 36 publications

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Effect of supplementation of different growth factors in embryo culture medium with a small number of bovine embryos on in vitro embryo development and quality

Urokinase-type plasminogen activator does not affect in vitro bovine embryo development and quality

Producción de embriones bovinos (Bos taurus) in vitro en co-cultivo a partir de ovocitos vitrificados en estadío maduro

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