Effect of culture time on secondary metabolite production from MeJA-stimulated in vitro roots of Hyoscyamus niger L.

Abstract

This study was conducted to determine the effects of different culture times on root growth and accumulation of secondary metabolites in adventitious roots from Hyoscyamus niger petioles. For this purpose, adventitious roots transferred to culture media containing 1 mM methyl jasmonate (MeJA) were harvested at 11 different culture times, starting from day 1 to day 30. After harvest, the fresh and dry weights of the roots and the root growth index as parameters of root growth, as well as the amounts of tropane alkaloids and phenolic compounds, were examined. It was found that the fresh and dry weights and the growth index of the roots harvested between the 12th and 30th day were the highest. The highest amounts of scopolamine were obtained from roots harvested on days 15, 18, and 21. The highest hyoscyamine accumulation was found on days 12 and 15. The highest total phenolic content was 19.33 mg g-1 in root cultures harvested on day 15. HPLC analyses revealed that ferulic acid, caffeic acid, o-coumaric acid, p-coumaric acid, rutin, and quercetin were not detected in the roots. On the other hand, the amounts of gallic acid, catechin, epicatechin, vanillin, cinnamic acid, rosmarinic acid, and chlorogenic acid showed significant changes depending on the culture times. Considering all the results, it was found that the optimum culture time for the production of secondary metabolites in H. niger adventitious root cultures stimulated with 1mM MeJA was 15 days.

In vitro production of steroidal saponin, total phenols and antioxidant activity in callus suspension culture of Paris polyphylla Smith: an important Himalayan medicinal plant

“…According to the culture method, Wang et al (2022) recently observed that the MeJA-treated Chinese chives had substantial variations in primary and secondary metabolites. Similar outcomes were also reported by other researchers, who claimed that using MeJA as an elicitor in the culture increased the synthesis of the secondary metabolites (Rodrıǵuez-Sańchez et al, 2020;Deveci et al, 2022;Wang et al, 2022).…”

Prediction of the concentration of plant growth regulators for somatic embryogenesis and regeneration of Hyoscyamus niger using Box–Behnken design of response surface methodology

“…Despite the therapeutic effects, these compounds also have toxic effects on humans. Scopolamine and hyoscyamine can cause intoxication by paralyzing the nerve endings of the parasympathetic system (Deveci et al 2022).…”

Total Content of Saponins, Phenols and Flavonoids and Antioxidant and Antimicrobial Activity of In Vitro Culture of Allochrusa gypsophiloides (Regel) Schischk Compared to Wild Plants

“…The dependence of tropane alkaloid and phenol accumulation on the duration of cultivation and L-phenylalanine treatment in the in vitro AR culture of Hyoscyamus niger L. was shown [52]. The effect of the culture period on the yield of secondary metabolites with an optimal 15-day period is shown in the in vitro AR of Hyoscyamus niger L. [53]. The HPLC of the in vitro culture of Lang Bian ginseng showed that only twenty-week-old in vitro rhizomes had ginsenoside Rg1, Rd and Rb1 [54].…”