Effect of culture medium on biocalcification by Pseudomonas Putida, Lysinibacillus Sphaericus and Bacillus Subtilis

Abstract

The objective of this study is to investigate the efficiency of calcium carbonate bioprecipitation by Lysinibacillus sphaericus, Bacillus subtilis and Pseudomonas putida, obtained from the Coleção de Culturas do Instituto Nacional de Controle de Qualidade em Saúde (INCQS), as a first step in determining their potential to protect building materials against water uptake. Two culture media were studied: modified B4 containing calcium acetate and 295 with calcium chloride. Calcium consumption in the two media after incubation with and without the bacterial inoculum was determined by atomic absorption analysis. Modified B4 gave the best results and in this medium Pseudomonas putida INQCS 113 produced the highest calcium carbonate precipitation, followed by Lysinibacillus sphaericus INQCS 414; the lowest precipitation was produced by Bacillus subtilis INQCS 328. In this culture medium XRD analysis showed that Pseudomonas putida and Bacillus subtilis precipitated calcite and vaterite polymorphs while Lysinibacillus sphaericus produced only vaterite. The shape and size of the crystals were affected by culture medium, bacterial strain and culture conditions, static or shaken. In conclusion, of the three strains Pseudomonas putida INQCS 113 in modified B4 medium gave the best results precipitating 96% of the calcium, this strain thus has good potential for use on building materials

AlkaliphilicBacillusspecies show potential application in concrete crack repair by virtue of rapid spore production and germination then extracellular calcite formation

“…B4 medium has been used for screening bacteria for calcium carbonate precipitation (Shirakawa et al . ). Although B4 medium supported the greatest growth (Fig.…”

“…Bio‐calcification involves precipitation of calcium carbonate polymorphs and occurs commonly in soil, fresh water and marine environments (Shirakawa et al . ).…”

“…; Shirakawa et al . ). There are at least two pathways for microbial precipitation of CaCO 3 : passive and active.…”

Influence of Substrate Mineralogy on Bacterial Mineralization of Calcium Carbonate: Implications for Stone Conservation

“…This may explain why bacterial calcite can eventually form on quartz grains. This rationale may also help to explain why different carbonatogenic bacteria cultured in glass flasks (with the same growth medium) produce either vaterite or calcite plus vaterite (76).…”

Precipitated Calcium Carbonate Nano-Microparticles: Applications in Drug Delivery

“…Researchers reported the same result with marine yeast, and claimed that calcium crystals resulted from accumulation of calcium deposits on the surface of cells in the aragonite structure. 43,44 Rivadeneyra et al 26 presented an article in which they studied precipitation of calcium carbonate by Deleya hatophila using solids. It was studied in liquid media different concentrations of NaCl as the single salt and different incubation temperatures.…”