Effect of culture medium on the production and virulence of submerged spores ofMetarhizium anisopliaeandBeauveria bassianaagainst larvae and adults ofAedes aegypti(Diptera: Culicidae)

Maldonado-Blanco, María Guadalupe; Gallegos-Sandoval, Johanna Lizzette; Fernández-Peña, Gabriela; Sandoval-Coronado, Carlos Francisco; Elías-Santos, Myriam

doi:10.1080/09583157.2013.855164

Cited by 11 publications

(6 citation statements)

References 20 publications

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“…The influence of additives such as casamino acid on the spore production and virulence of various fungal isolates has been reported to vary among different media. For example, a M. anisopliae isolate cultured in medium with added casamino acids showed increased 40% mortality against A. aegypti compared with the same isolate cultured in medium without added casamino acids [ 21 ]. According to Shah et al [ 24 ], M. anisopliae produced highly virulent conidia when cultivated in osmotic stress medium, and the conidia showed higher Pr1 enzyme activity.…”

Section: Discussionmentioning

confidence: 99%

“…For example, additives such as lecithin, collagen, or lactic acid into culture broth increased the production of Metarhizium anisopliae blastospores, but the virulence of the spores was weaker than that of spores produced on media without additive [ 19 ]. The addition of casamino acid into culture broth increased the production yield of M. anisopliae spores and improved their virulence against Aedes aegypti [ 21 ]. Addition of yeast extract and peptone into liquid media improved both the production of Beauveria brongniartii spores and their virulence against T. vaporariorum [ 22 ].…”

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confidence: 99%

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Influence of Additives on the Yield and Pathogenicity of Conidia Produced by Solid State Cultivation of an Isaria javanica Isolate

et al. 2014

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Recently, the Q biotype of tobacco whitefly has been recognized as the most hazardous strain of Bemisia tabaci worldwide, because of its increased resistance to some insecticide groups. As an alternative control agent, we selected an Isaria javanica isolate as a candidate for the development of a mycopesticide against the Q biotype of sweet potato whitefly. To select optimal mass production media for solid-state fermentation, we compared the production yield and virulence of conidia between 2 substrates (barley and brown rice), and we also compared the effects of various additives on conidia production and virulence. Barley was a better substrate for conidia production, producing 3.43 × 1010 conidia/g, compared with 3.05 × 1010 conidia/g for brown rice. The addition of 2% CaCO3 + 2% CaSO4 to barley significantly increased conidia production. Addition of yeast extract, casein, or gluten also improved conidia production on barley. Gluten addition (3% and 1.32%) to brown rice improved conidia production by 14 and 6 times, respectively, relative to brown rice without additives. Conidia cultivated on barley produced a mortality rate of 62% in the sweet potato whitefly after 4-day treatment, compared with 53% for conidia cultivated on brown rice. The amendment of solid substrate cultivation with additives changed the virulence of the conidia produced; the median lethal time (LT50) was shorter for conidia produced on barley and brown rice with added yeast extract (1.32% and 3%, respectively), KNO3 (0.6% and 1%), or gluten (1.32% and 3%) compared with conidia produced on substrates without additives.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Influence of Additives on the Yield and Pathogenicity of Conidia Produced by Solid State Cultivation of an Isaria javanica Isolate

et al. 2014

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“…For the conidia production, it was found that the type of culture medium used to produce M. anisopliae is capable of affecting its virulence. A study found that the virulence of the different strains was influenced by different medium cultures [ 69 ]. This may be due to the different nutrients required by different strains of M. anisopliae that are genetically different.…”

Section: Mass Production Of Metarhizium Anisopliae mentioning

confidence: 99%

“…This may be due to the different nutrients required by different strains of M. anisopliae that are genetically different. In addition, Maldonado-Blanco et al [ 69 ] also found that the mortality of Aedes spp. increases with an increasing incubation time with the spores.…”

Section: Mass Production Of Metarhizium Anisopliae mentioning

confidence: 99%

Mode of Infection of Metarhizium spp. Fungus and Their Potential as Biological Control Agents

Hue

2017

JoF

101

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Chemical insecticides have been commonly used to control agricultural pests, termites, and biological vectors such as mosquitoes and ticks. However, the harmful impacts of toxic chemical insecticides on the environment, the development of resistance in pests and vectors towards chemical insecticides, and public concern have driven extensive research for alternatives, especially biological control agents such as fungus and bacteria. In this review, the mode of infection of Metarhizium fungus on both terrestrial and aquatic insect larvae and how these interactions have been widely employed will be outlined. The potential uses of Metarhizium anisopliae and Metarhizium acridum biological control agents and molecular approaches to increase their virulence will be discussed.

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“…Tabla 7.2.Por ejemplo, el medio de cultivo es un factor a considerar en este aspecto ya que, de acuerdo aMaldonado-Blanco et al, (2014), los conidios sumergidos de M. anisopliae producidos en medio con casaminoácidos (caseína hidrolizada y aminoácidos) incrementaron su virulencia hacia larvas del mosquito Aedes aegypti, aunque no se encontró el mismo efecto en los conidios de B. bassiana. Otra investigación donde también se evidencia ésto, es la realizada por Ortiz-Urquiza et al, (2013), en la cual se evalúan cuatro medios de cultivo para M. anisopliae encontrándose que, al crecer este hongo en algunos de ellos, se incrementa su infectividad y virulencia hacia larvas de G. mellonella y adultos de Ceratitis capitata.…”

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Producción de conidios de hongos entomopatógenos en estado oxidante y análisis de su respuesta a distintos tipos de estrés

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Effect of culture medium on the production and virulence of submerged spores ofMetarhizium anisopliaeandBeauveria bassianaagainst larvae and adults ofAedes aegypti(Diptera: Culicidae)

Cited by 11 publications

References 20 publications

Influence of Additives on the Yield and Pathogenicity of Conidia Produced by Solid State Cultivation of an Isaria javanica Isolate

Influence of Additives on the Yield and Pathogenicity of Conidia Produced by Solid State Cultivation of an Isaria javanica Isolate

Mode of Infection of Metarhizium spp. Fungus and Their Potential as Biological Control Agents

Producción de conidios de hongos entomopatógenos en estado oxidante y análisis de su respuesta a distintos tipos de estrés

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