Effect of culture media on embryo development from prepubertal goat IVM-IVF oocytes

Izquierdo, Dolors; Villamediana, Patricia; Paramio, M. T.

doi:10.1016/s0093-691x(99)00177-6

Cited by 51 publications

(37 citation statements)

References 64 publications

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“…Furthermore, abattoir derived prepubertal ovaries constitute an economical source of oocytes for embryo biotechnology. However, although oocytes obtained from prepubertal animals have produced viable offspring after in vitro maturation and fertilization [2], [3], [4], [5], the developmental competence of these embryos was lower than that of embryos produced from adult animals [2], [3], [6]–[13].…”

Section: Introductionmentioning

confidence: 94%

Developmental Potential of Prepubertal Mouse Oocytes Is Compromised Due Mainly to Their Impaired Synthesis of Glutathione

et al. 2013

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Although oocytes from prepubertal animals are found less competent than oocytes from adults, the underlying mechanisms are poorly understood. Using the mouse oocyte model, this paper has tested the hypothesis that the developmental potential of prepubertal oocytes is compromised due mainly to their impaired potential for glutathione synthesis. Oocytes from prepubertal and adult mice, primed with or without eCG, were matured in vitro and assessed for glutathione synthesis potential, oxidative stress, Ca2+ reserves, fertilization and in vitro development potential. In unprimed mice, abilities for glutathione synthesis, activation, male pronuclear formation, blastocyst formation, cortical granule migration and polyspermic block were all compromised significantly in prepubertal compared to adult oocytes. Cysteamine and cystine supplementation to maturation medium significantly promoted oocyte glutathione synthesis and blastocyst development but difference due to maternal age remained. Whereas reactive oxygen species (ROS) levels increased, Ca2+ storage decreased significantly in prepubertal oocytes. Levels of both catalytic and modifier subunits of the γ-glutamylcysteine ligase were significantly lower in prepubertal than in adult oocytes. Maternal eCG priming improved all the parameters and eliminated the age difference. Together, the results have confirmed our hypothesis by showing that prepubertal oocytes have a decreased ability to synthesize glutathione leading to an impaired potential to reduce ROS and to form male pronuclei and blastocysts. The resulting oxidative stress decreases the intracellular Ca2+ store resulting in impaired activation at fertilization, and damages the microfilament network, which affects cortical granule redistribution leading to polyspermy.

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Section: Introductionmentioning

confidence: 94%

Developmental Potential of Prepubertal Mouse Oocytes Is Compromised Due Mainly to Their Impaired Synthesis of Glutathione

et al. 2013

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“…Although oocytes obtained from prepubertal animals have produced viable offspring after in vitro maturation and IVF (Revel et al 1995, O'Brien et al 1997, Khatir et al 1998a, Ptak et al 1999, the developmental competence of these embryos was lower than that of embryos produced from adult animals (Seidel et al 1971, Pinkert et al 1989, Revel et al 1995, Damiani et al 1996, Khatir et al 1996, O'Brien et al 1996, Ledda et al 1997, Izquierdo et al 1999, Wu et al 2007. In vitro matured oocytes of prepubertal bovine showed abnormal chromatin microtubule configurations and protein synthesis (Damiani et al 1996, Gandolfi et al 1998, Khatir et al 1998b).…”

Section: Introductionmentioning

confidence: 99%

Effects of puberty and gonadotropins on the molecular events controlling meiotic resumption of mouse oocytes

Han¹,

Cao²,

Wang³

et al. 2010

Reproduction

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Although studies suggest that the low competence of oocytes from prepubertal animals is due to their insufficient cytoplasmic maturation and that FSH improves oocyte maturation possibly by retarding meiotic progression and allowing more time for cytoplasmic maturation, the mechanisms by which puberty and gonadotropins regulate meiotic progression require additional detailed studies. For the first time, we observed that while meiotic progression was significantly slower, the maturation-promoting factor (MPF) activity of oocytes was significantly higher in prepubertal than in adult mice. To resolve this contradiction, we specified the molecules regulating the MPF activity and their localization during oocyte maturation in prepubertal and adult mice primed with or without gonadotropins. Our tests using corresponding enzyme regulators suggested that while activities of protein kinase A were unaffected, the activity of adenylate cyclase (ADCY) and phosphodiesterase increased while cell division cycle 2 homolog A (CDC2A) decreased significantly after puberty. While most of the adult oocytes had CDC2A protein concentrated in the germinal vesicle (GV) region, the majority of prepubertal oocytes showed no nuclear concentration of CDC2A. Maximally priming mice with equine chorionic gonadotropin brought the above parameters of prepubertal oocytes close to those in adult oocytes. Together, the results suggest that puberty and gonadotropin control oocyte meiotic progression mainly by regulating the ADCY activity and the concentration of the activated MPF toward the GV region.

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“…However, it has been found that immature oocytes from cows with reduced reproductive performance or slaughtered at the end of their use are heterogeneous in quality [9]. The developmental capacity of oocytes from pre-pubertal animals was lower than that of oocytes from adult animals (cattle: [14][15][16][17]; sheep: [18,19]; pig: [20]; goat: [21,22]). Follicular waves and advanced atresia of the ovary also affected the developmental competence of oocytes [11,23].…”

Section: Introductionmentioning

confidence: 99%

Selection of oocytes for in vitro maturation by brilliant cresyl blue staining: a study using the mouse model

Liu

Zhou

et al. 2007

Cell Res

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Effect of culture media on embryo development from prepubertal goat IVM-IVF oocytes

Cited by 51 publications

References 64 publications

Developmental Potential of Prepubertal Mouse Oocytes Is Compromised Due Mainly to Their Impaired Synthesis of Glutathione

Developmental Potential of Prepubertal Mouse Oocytes Is Compromised Due Mainly to Their Impaired Synthesis of Glutathione

Effects of puberty and gonadotropins on the molecular events controlling meiotic resumption of mouse oocytes

Selection of oocytes for in vitro maturation by brilliant cresyl blue staining: a study using the mouse model

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