Effect of culture conditions on astaxanthin production by a mutant of Phaffia rhodozyma in batch and chemostat culture

Transport-limited sucrose utilization and neokestose production by Phaffia rhodozyma

“…1), confirming previous results (Meyer and du Preez, 1994). In addition, however, HPLC analysis indicated the accumulation of an unknown compound in the medium (Fig.…”

“…Utilization of sucrose by this yeast, however, results in the temporary accumulation of glucose and fructose in the medium, indicating that glucose and fructose transport limits the sucrose utilization rate (Meyer and du Preez, 1994). This prompted us to investigate the utilization of sucrose by P. rhodozyma in relation to the rates of transport of its monomers.…”

Effect of acetic acid on astaxanthin production by Phaffia rhodozyma

“…Batch cultivations were conducted in a 2 1 glass fermenter at 22°C and pH 5 as described by Meyer and Du Preez (1993b). The pH was automatically controlled at pH 5, initially with 3 N NaOH and after sugar depletion with filter sterilized acetic acid.…”

Perfusion culture process plus H2O2 stimulation for efficient astaxanthin production by Xanthophyllomyces dendrorhous

“…Various operation modes, including batch, fed-batch, and continuous, have been studied for astaxanthin production with X. dendrorhous (An et al, 1996;Ducrey Santopietro et al, 1998;Meyer and Du Preez, 1994;Yamane et al, 1997). Batch culture process usually gives the lowest biomass and product yields because of rapid nutrient (sugar) depletion and inhibitory metabolite accumulation.…”

“…Batch culture process usually gives the lowest biomass and product yields because of rapid nutrient (sugar) depletion and inhibitory metabolite accumulation. Continuous operation is less favorable for commercial bioprocesses in general and for the X. dendrorhous cultures in particular, owning to their relatively low specific cell growth rates (Meyer and Du Preez, 1994) and non-growth-associated astaxanthin accumulation . Although fedbatch process is generally more favorable than batch or continuous process, the yeast growth and astaxanthin production could be inhibited by waste metabolites accumulating to high levels in later stage of the culture process.…”