Effect of culture conditions, cytokinins, methyl jasmonate and salicylic acid on the biomass accumulation and production of withanolides in multiple shoot culture of Withania somnifera (L.) Dunal using liquid culture

Sivanandhan, Ganeshan; Rajesh, Manoharan; Arun, Muthukrishnan; Jeyaraj, Murugaraj; Dev, Gnanajothi Kapil; Annavi, Arjunan; Manickavasagam, Markandan; Muthuselvam, Manickam; Selvaraj, N.; Ganapathi, A.

doi:10.1007/s11738-012-1112-x

Cited by 60 publications

(24 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both parameters need to be optimized for each individual compound. This conclusion agrees with these reached for several other metabolites and in vitro culture systems (Namdeo, 2007;Sivanandhan et al, 2013). Among concentrations tested, 150 μM MeJa was the best for biosynthesis of iridoids (catalpol, harpagide) (Fig.…”

Section: Results and Discussion Growth Kinetics In Shake Flaskssupporting

confidence: 89%

The Influence of Methyl Jasmonate and Salicylic Acid on Secondary Metabolite Production in Rehmannia Glutinosa Libosch. Hairy Root Culture

Piątczak¹,

Kuźma²,

Wysokińska³

2016

Acta Biologica Cracoviensia S. Botanica

View full text Add to dashboard Cite

Rehmannia glutinosa hairy roots were used to evaluate the effect of methyl jasmonate (MeJa) and salicylic acid (SA) on increase of root biomass and production of iridoids (catalpol, harpagide) and phenylethanoids (verbascoside and isoverbascoside). The elicitors were added to 23-day-old culture separately at concentrations between 50 and 200 μM or in combinations at concentrations of 50 and 100 μM. Roots were harvested 72 h and 120 h after elicitation. The type of elicitor, its concentration and exposure time were found to strongly affect the content of each analyzed compound. A 72-hour treatment with 200 μM MeJa was the most effective in increase of verbascoside content (60.07 mg·DW -1 equivalent to 845.45 mg·L -1 ) and isoverbascoside (1.77 mg·DW -1 equivalent to 24.94 mg·L -1 ): these respective amounts were roughly 10-and 6.4-fold higher than the control values (unelicited roots). Exposure to 150 μM MeJa provided optimal harpagide content after 72 hours (0.136 mg·DW -1 ; 7.5-fold increase compared to the control), and catalpol content after 120 hours (up to 2.145 mg·DW -1 ). The combination of MeJa and SA also resulted in higher levels of secondary metabolites compared to the control culture, although these levels were lower than those observed for MeJa alone at the optimal concentration and exposure time. SA alone was less efficient in enhancing metabolite production than MeJa.

show abstract

Section: Results and Discussion Growth Kinetics In Shake Flaskssupporting

confidence: 89%

The Influence of Methyl Jasmonate and Salicylic Acid on Secondary Metabolite Production in Rehmannia Glutinosa Libosch. Hairy Root Culture

Piątczak¹,

Kuźma²,

Wysokińska³

2016

Acta Biologica Cracoviensia S. Botanica

View full text Add to dashboard Cite

show abstract

“…Kaminek (1992) stated that changes in the morphogenic responses of cytokinins like BA can be clarified by their several translocation rates to meristematic regions and metabolic process, in which the cytokinins may be degraded and conjugated with physiologically inert compounds. Similarly, BA has extensively influenced the multiple shoot regeneration efficiency in Chrysanthemum morifolium (Song et al 2011), Withania somnifera (Sivanandhan et al 2011;Sivanandhan et al 2013) and Naravelia zeylanica (Benson et al 2014). Multiple shoot production rates stated in the present study are apparently very high 3.5-and 2.25-fold from leaf and nodal explants respectively when compared to those reported by previous investigations; Prakash et al (1999) obtained maximum 11 shoots from seed-derived light-green compact callus of H. enneaspermus on MS medium containing 1.86 mM KH 2 PO 4 , 500 mg/l casein hydrolysate, 8.8 μM BA and 2.6 μM NAA.…”

Section: Resultsmentioning

confidence: 99%

“…Exogenously applied cytokinins alter the development of axillary meristems, promote proliferation of the meristematic cells in the axillary buds and increase the number of bud primordia from the pre-existing meristems (Carmen et al 2001). Sivanandhan et al (2013) reported that the nutrient components in the medium and exogenously added cytokinins stimulated and favoured the development of growth of multiple axillary buds and two to three fold enhancement in shoot production can be fabulously attained within a shorter period under optimized culture conditions. In the present investigation, increased multiple shoot production was noticed at all cytokinin concentrations tested with the exclusion of treatments with 2, iP for both types of explants.…”

Section: Resultsmentioning

confidence: 99%

“…The hormonal treatment optimised in the current experiment provided a 1.1-and 1.6-fold increment in the number of regenerated shoots from leaf and nodal explants, respectively when compared to the highest number perceived in the present experiment in treatments with cytokinin alone. Other literatures have correspondingly reported the synergistic/additive effect of auxins with cytokinins on shoot proliferation in many medicinal plants, for instance W. somnifera and Aloe arborescens (Sivanandhan et al 2011;Amoo et al 2012;Sivanandhan et al 2013). …”

Section: Optimization Of Cytokinin Interaction With Auxins On Multiplmentioning

confidence: 99%

See 1 more Smart Citation

L-Dopa production and antioxidant activity in Hybanthus enneaspermus (L.) F. Muell regeneration

Sivanandhan

Vasudevan

Selvaraj³

et al. 2015

Physiol Mol Biol Plants

Self Cite

View full text Add to dashboard Cite

Hybanthus enneaspermus is an ethanobotanical plant extensively used in Indian traditional medicine. Quick and efficient in vitro mass propagation of this plant species was established for commercial utilization from leaf and node explants using various concentrations and combinations of plant growth regulators and polyamines. The maximum number of multiple shoots per leaf explant (40 shoots) was achieved on MS medium supplemented with 20 mg/l spermidine in combination with 4 mg/l BA+1.5 mg/l IAA after 8 weeks of culture. The elongated shoots were rooted (16 roots/shoot) on MS medium with the best concentration of IBA (1.5 mg/l) and in combination with 20 mg/l putrescine after 5 weeks of culture. The plants were successfully acclimatized (98 %) in the sand: soil: vermiculite mixture (1:1:1 v/v/v) in the greenhouse. An increased antioxidant activity was recorded in vitro regenerated shoots when compared to in vitro-induced roots. L-Dopa content was recorded higher in leaves (8.31 mg/g DW) followed by stem (6.22 mg/g DW) and root (3.22 mg/g DW) of leaf-derived plants than the fieldgrown parent plant after 5 weeks. By adopting this protocol, the regenerated-plants could be used for drug production and pharmacology work with as an alternative to field-grown plants.

show abstract

“…Elite cell lines isolated from natural population can be genetically more stable and suitable for large scale production of bioactive compounds. Various in vitro culture methods, including callus culture [10], adventitious shoot culture [11] and cell culture systems [12] have been adapted for the production of therapeutically valuable withanolide compounds from W. somnifera. Hairy roots are considered a very good system for continuous synthesis of valuable metabolic compounds in an aseptic condition in the absence of expensive growth regulators in the culture medium [13,14].…”

Section: Introductionmentioning

confidence: 99%

Efficient Agrobacterium Rhizogenes Mediated Hairy Root Culture System for Withanolide Production from Withania Somnifera (L.) Dunal

Cs¹,

Aslam²,

K³

et al. 2017

JABB

View full text Add to dashboard Cite

Withania somnifera (L.) Dunal is an important medicinal plant with many pharmaceutical and therapeutic uses ranging from immunomodulation to anticancer property. Field cultivation of W. somnifera is time consuming and it not able to meet current global demands. For commercial production of withanolides, field grown plant materials have generally been used but the quality and uniformity in the levels of active constituents are highly affected by genotype and environmental conditions. In vitro culture methods are the best alternative and have possible ways to enhance commercial production of withanolides. In this review, we summarize our recent findings on characterization and screening of wild accessions of W. somnifera collected from Tamil Nadu state. Further, an improved Agrobacterium rhizogenes mediated hairy root culture system was developed and this system providing an efficient tool attaining better transformation efficiency and useful for commercial in vitro production of withanolides.

show abstract

Effect of culture conditions, cytokinins, methyl jasmonate and salicylic acid on the biomass accumulation and production of withanolides in multiple shoot culture of Withania somnifera (L.) Dunal using liquid culture

Cited by 60 publications

References 33 publications

The Influence of Methyl Jasmonate and Salicylic Acid on Secondary Metabolite Production in Rehmannia Glutinosa Libosch. Hairy Root Culture

The Influence of Methyl Jasmonate and Salicylic Acid on Secondary Metabolite Production in Rehmannia Glutinosa Libosch. Hairy Root Culture

L-Dopa production and antioxidant activity in Hybanthus enneaspermus (L.) F. Muell regeneration

Efficient Agrobacterium Rhizogenes Mediated Hairy Root Culture System for Withanolide Production from Withania Somnifera (L.) Dunal

Contact Info

Product

Resources

About