Effect of cultivation parameters on the production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) and poly(3-hydroxybutyrate-4-hydroxybutyrate-3-hydroxyvalerate) by Cupriavidus necator using waste glycerol

Cavalheiro, João; Raposo, Rita; Almeida, M. Catarina M.D. de; Cesário, M. Teresa; Sevrin, Chantal; Grandfils, Christian; Fonseca, M. Manuela R. da

doi:10.1016/j.biortech.2012.01.176

Cited by 157 publications

(96 citation statements)

References 24 publications

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“…16.7 g L -1 terpolymer concentration with a PHA content of 37 % dcw was reported at 20 % dissolved oxygen concentration. 73 Fed-batch cultivation using crude glycerol and rapeseed meal hydrolysate by C. necator DSM 545 demonstrated a two-fold increase in production of P(3HB-co-3HV) copolymer to 10.9 g L -1 as compared to a polymer concentration of 5.5 g L -1 obtained in batch cultivation. 54 Production of a PHA copolymer comprising predominantly 4 mcl-PHA monomer units was reported using an isolated thermotolerant Pseudomonas SG4502 strain when grown on biodiesel waste.…”

Section: Fed-batch Fermentationmentioning

confidence: 95%

Strategies for Large-scale Production of Polyhydroxyalkanoates

Kaur

Roy

2015

Chem.Biochem.Eng.Q.

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Polyhydroxyalkanoates (PHAs) are biodegradable and biocompatible intracellular polyesters that are accumulated as energy and carbon reserves by bacterial species, under nutrient limiting conditions. Successful large-scale production of PHAs is dependent on three crucial factors, which include the cost of substrate, downstream processing cost, and process development. In this respect, design and implementation of bioprocess strategies for efficient PHA bioconversions enable high PHA concentrations, yields and productivities. Additionally, development of PHA fermentation processes using inexpensive substrates, such as agro-industrial wastes, facilitates further cost reduction, thus benefitting large-scale PHA production. Thus, the aim of this review is to highlight various bioprocess strategies for high production of PHAs and their novel copolymers in relatively large quantities. This review also discusses the application of kinetic analysis and mathematical modelling as important tools for process optimization and thus improvement of the overall process economics for large-scale production of PHAs.

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Section: Fed-batch Fermentationmentioning

confidence: 95%

Strategies for Large-scale Production of Polyhydroxyalkanoates

Kaur

Roy

2015

Chem.Biochem.Eng.Q.

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“…The production of different kinds of PHAs by C. necator using several inexpensive feedstock, including glycerol, has been recently reported 18,19,20,21,22 , indicating that some strains of this bacterial species could be used for the conversion of fatty residues into PHA. As an alternative, the use of costly commercial enzymes or genetic modification of microorganisms exhibiting high PHA product yields would be required 23 .…”

Section: Introductionmentioning

confidence: 99%

Poly(hydroxyalkanoate) Production by Cupriavidus necator from Fatty Waste Can Be Enhanced by phaZ1 Inactivation

Povolo

Basaglia

Fontana

et al. 2015

Chem.Biochem.Eng.Q.

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PHA production from waste oils or fats requires microorganisms that should be both excellent PHA producers and equipped with enzymatic activities allowing hydrolysation of triglycerides. Unfortunately, microbes with the combination of substrate-utilization and PHA production are not currently available, and the strategies to be adopted are the use of costly commercial enzymes, or genetic modification of microorganisms exhibiting high PHA product yields.In the present work, after a general investigation on the ability of Cupriavidus necator to grow on a number of fatty substrates, the possibility to enhance PHA production by limiting intracellular depolymerisation, was investigated. By knocking out the related phaZ1 gene, the construction of C. necator recombinant strains impaired in depolymerase (PhaZ1) activity was achieved. The polymer yield of the recombinant strain was finally compared to that of the parental C. necator DSM 545.

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“…Examples are Cupriavidus necator former Ralstonia eutropha [11][12], Alcaligenes latus [13][14], Comamonas acidovorans ( [14][15][16], Comamonas testosteronii [17] and Hydrogenophaga pseudoflava [18]. Recombinant E. coli has also been used to obtain P(3HB-co-4HB) with 2.8 mol% 4HB [19] as well as to produce the homopolymer P(4HB) [10], for medical purposes at Tepha Inc.…”

Section: Introductionmentioning

confidence: 99%

Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by Burkholderia sacchari using wheat straw hydrolysates and gamma-butyrolactone

Cesário

Raposo

Almeida

et al. 2014

International Journal of Biological Macromolecules

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Burkholderia sacchari DSM 17165 is able to grow and produce poly(3-hydroxybutyrate) both on hexoses and pentoses. In a previous study, wheat straw lignocellulosic hydrolysates (WSH) containing high C6 and C5 sugar concentrations were shown to be excellent carbon sources for P(3HB) production.Using a similar feeding strategy developed for P(3HB) production based on WSH, fedbatch cultures were developed aiming at the production of the copolymer P(3HB-co-4HB) (poly(3-hydroxybutyrate-co-4-hydroxybutyrate)) by B. sacchari. The ability of this * Corresponding author. Tel.: +351 21 8419137; Fax: +351 21 8419062.e-mail address: teresa.cesario@tecnico.ulisboa.pt 2 strain to synthesize P(3HB-co-4HB) was first shown in shake flasks using gammabutyrolactone (GBL) as precursor of the 4HB units.Fed-batch cultures using glucose as carbon source (control) and GBL were developed to achieve high copolymer productivities and 4HB incorporations. The attained P(3HB-co-4HB) productivity and 4HB molar % were 0.7 g/(L·h) and 4.7 molar %, respectively.The 4HB incorporation was improved to 6.3 and 11.8 molar % by addition of 2 g/L propionic and acetic acid, respectively. When WSH were used as carbon source under the same feeding conditions, the values achieved were 0.5 g/(L·h) and 5.0 molar %, respectively. Burkholderia sacchari, a strain able to produce biopolymers based on xylose-rich lignocellulosic hydrolysates, is for the first time reported to produce P(3HB-co-4HB) using gamma butyrolactone as precursor.

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Effect of cultivation parameters on the production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) and poly(3-hydroxybutyrate-4-hydroxybutyrate-3-hydroxyvalerate) by Cupriavidus necator using waste glycerol

Cited by 157 publications

References 24 publications

Strategies for Large-scale Production of Polyhydroxyalkanoates

Strategies for Large-scale Production of Polyhydroxyalkanoates

Poly(hydroxyalkanoate) Production by Cupriavidus necator from Fatty Waste Can Be Enhanced by phaZ1 Inactivation

Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) by Burkholderia sacchari using wheat straw hydrolysates and gamma-butyrolactone

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