Effect of CTAB and CTAB in the presence of hyaluronan on selected human cell types

Kalbáčová, Marie Hubálek; Verdanova, Martina; Mravec, Filip; Halasová, Tereza; Pekař, Miloslav

doi:10.1016/j.colsurfa.2013.12.048

Cited by 21 publications

(8 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…CTAB and MKC are quaternary ammonium surfactants, and their cytotoxicity to different human cells was previously reported. 34 , 35 The results show that the effect of DiC18 loading is negligible, and the cytotoxicity is governed by the concentration of the surfactant (CTAB or MKC). Good viability was obtained for C-QS at a concentration of 0.022 mM CTAB and for M-QS at a concentration of 0.044 mM MKC, respectively.…”

Section: Resultsmentioning

confidence: 94%

Fluorenyl-Loaded Quatsome Nanostructured Fluorescent Probes

et al. 2017

View full text Add to dashboard Cite

Delivery of hydrophobic materials in biological systems, for example, contrast agents or drugs, is an obdurate challenge, severely restricting the use of materials with otherwise advantageous properties. The synthesis and characterization of a highly stable and water-soluble nanovesicle, referred to as a quatsome (QS, vesicle prepared from cholesterol and amphiphilic quaternary amines), that allowed the nanostructuration of a nonwater soluble fluorene-based probe are reported. Photophysical properties of fluorenyl–quatsome nanovesicles were investigated via ultraviolet–visible absorption and fluorescence spectroscopy in various solvents. Colloidal stability and morphology of the nanostructured fluorescent probes were studied via cryogenic transmission electronic microscopy, revealing a “patchy” quatsome vascular morphology. As an example of the utility of these fluorescent nanoprobes, examination of cellular distribution was evaluated in HCT 116 (an epithelial colorectal carcinoma cell line) and COS-7 (an African green monkey kidney cell line) cell lines, demonstrating the selective localization of C-QS and M-QS vesicles in lysosomes with high Pearson’s colocalization coefficient, where C-QS and M-QS refer to quatsomes prepared with hexadecyltrimethylammonium bromide or tetradecyldimethylbenzylammonium chloride, respectively. Further experiments demonstrated their use in time-dependent lysosomal tracking.

show abstract

Section: Resultsmentioning

confidence: 94%

Fluorenyl-Loaded Quatsome Nanostructured Fluorescent Probes

et al. 2017

View full text Add to dashboard Cite

show abstract

“…The cytotoxicity of CTAB in human keratinocytes (HaCaT) and osteoblast-like (SAOS-2) cell lines, in the presence or absence of serum (FBS), was shown to be CTAB dose-dependent and was affected by the presence of FBS which clearly showed cell protection [15]. In the absence of FBS (the same conditions in which our incubations were performed), 200 µM (72.88 µg/mL) of surfactant reduced the metabolic activity of cells (SAOS-2 to~20% and HaCaT to less than 10%), although in live/dead assays, at 200 µM only a slight decrease in SAOS-2 cells viability was observed [15]. Although the metabolic activity is low, the percentage of dead cells is still low, suggesting that, after 24 h incubation, cells still trigger the apoptotic events.…”

Section: Cell Linementioning

confidence: 99%

“…However, the interaction of cationic SLNs with the cells may be followed by some degree of toxicity. Indeed, it has been documented that non-ionic surfactants are less toxic, than ionic surfactants [3,15], while cationic surfactants have been reported to produce higher toxicity, as a result of their higher interaction with cell membranes [3,14,16]. Among cationic surfactants, CTAB (cetyltrimethylammonium bromide) and DDAB (dimethyldioctadecylammonium bromide) have been used in various industrial processes (e.g., as biocides and antiseptic agents [17]), and in the production of drug-loaded nanoparticles for various applications [4,[18][19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Soft Cationic Nanoparticles for Drug Delivery: Production and Cytotoxicity of Solid Lipid Nanoparticles (SLNs)

et al. 2019

View full text Add to dashboard Cite

The surface properties of nanoparticles have decisive influence on their interaction with biological barriers (i.e., living cells), being the concentration and type of surfactant factors to have into account. As a result of different molecular structure, charge, and degree of lipophilicity, different surfactants may interact differently with the cell membrane exhibiting different degrees of cytotoxicity. In this work, the cytotoxicity of two cationic solid lipid nanoparticles (SLNs), differing in the cationic lipids used as surfactants CTAB (cetyltrimethylammonium bromide) or DDAB (dimethyldioctadecylammonium bromide), referred as CTAB-SLNs and DDAB-SLNs, respectively, was assessed against five different human cell lines (Caco-2, HepG2, MCF-7, SV-80, and Y-79). Results showed that the cationic lipids used in SLN production highly influenced the cytotoxic profile of the particles, with CTAB-SLNs being highly cytotoxic even at low concentrations (IC50 < 10 µg/mL, expressed as CTAB amount). DDAB-SLNs produced much lower cytotoxicity, even at longer exposure time (IC50 from 284.06 ± 17.01 µg/mL (SV-80) to 869.88 ± 62.45 µg/mL (MCF-7), at 48 h). To the best of our knowledge, this is the first report that compares the cytotoxic profile of CTAB-SLNs and DDAB-SLNs based on the concentration and time of exposure, using different cell lines. In conclusion, the choice of the right surfactant for biological applications influences the biocompatibility of the nanoparticles. Regardless the type of drug delivery system, not only the cytotoxicity of the drug-loaded nanoparticles should be assessed, but also the blank (non-loaded) nanoparticles as their surface properties play a decisive role both in vitro and in vivo.

show abstract

“…Since Saos2 are sensible to CTAB in the range 0.2-2 mM, samples at lower concentration of CTAB, 7 µM and 0.7 µM , were tested corresponding to probe concentrations of 10 nM and 1 nM, respectively. 39 Other cell types, like HeLa cells, exhibit a decrease in cell viability only at higher concentrations of different quaternary ammonium surfactants. 40 PLAIN QS are more cytotoxic than CTAB, but PLAIN CHEMS QS are almost less cytotoxic than both CTAB and PLAIN QS .…”

Section: Biocompatibility Assays Of Dpp-loaded Qss and Dpp Onpsmentioning

confidence: 99%

Highly Stable and Red‐Emitting Nanovesicles Incorporating Lipophilic Diketopyrrolopyrroles for Cell Imaging

Ardizzone

Blasi

Vona

et al. 2018

Chemistry A European J

View full text Add to dashboard Cite

Diketopyrrolopyrroles (DPPs) have recently attracted much interest as very bright and photostable red-emitting molecules. However, their tendency to form nonfluorescent aggregates in water through the aggregation-caused quenching (ACQ) effect is a major issue that limits their application under the microscope. Herein, two DPP molecules have been incorporated into the membrane of highly stable and water-soluble quatsomes (QS; nanovesicles composed of surfactants and sterols), which allow their nanostructuration in water and, at the same time, limits the ACQ effect. The obtained fluorescent organic nanoparticles showed superior structural homogeneity, along with long-term colloidal and optical stability. A thorough one- (1P) and two-photon (2P) fluorescence characterization revealed the promising photophysical features of these fluorescent nanovesicles, which showed a high 1P and 2P brightness. Finally, the fluorescent QSs were used for the in vitro bioimaging of Saos-2 osteosarcoma cell lines; this demonstrates their potential as nanomaterials for bioimaging applications.

show abstract

Effect of CTAB and CTAB in the presence of hyaluronan on selected human cell types

Cited by 21 publications

References 15 publications

Fluorenyl-Loaded Quatsome Nanostructured Fluorescent Probes

Fluorenyl-Loaded Quatsome Nanostructured Fluorescent Probes

Soft Cationic Nanoparticles for Drug Delivery: Production and Cytotoxicity of Solid Lipid Nanoparticles (SLNs)

Highly Stable and Red‐Emitting Nanovesicles Incorporating Lipophilic Diketopyrrolopyrroles for Cell Imaging

Contact Info

Product

Resources

About