Effect of cryoprotectants on release of various enzymes from buck spermatozoa during freezing

Sirat, M.P.; Sinha, Archana; Singh, Brijesh Kumar; Prasad, R.

doi:10.1016/0093-691x(95)00377-k

Cited by 25 publications

(21 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Seminal plasma enzymes, such as ALT, AST, AP, LDH, and CPK are important for sperm metabolism and regulate sperm motility, fertility, and enhanced sperm survival and thus, have been used as diagnostic markers for semen quality [15], [28]. Although we did not find significant differences in AST, AP, or LDH between good and poor motility ejaculates, our results indicated that ALT levels were significantly higher in poor motility ejaculates and were inversely associated with sperm motility and Spermac positive staining.…”

Section: Discussionmentioning

confidence: 99%

“…Although we did not find significant differences in AST, AP, or LDH between good and poor motility ejaculates, our results indicated that ALT levels were significantly higher in poor motility ejaculates and were inversely associated with sperm motility and Spermac positive staining. ALT has also been used as a biomarker for cellular injury [28] and sperm membrane damage in other species including the ram [29] and rabbit [30]. It may therefore also be a useful indicator for acrosomal and/or sperm membrane integrity in Asian elephants.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Lactotransferrin in Asian Elephant (Elephas maximus) Seminal Plasma Correlates with Semen Quality

et al. 2013

View full text Add to dashboard Cite

Asian elephants (Elephas maximus) have highly variable ejaculate quality within individuals, greatly reducing the efficacy of artificial insemination and making it difficult to devise a sperm cryopreservation protocol for this endangered species. Because seminal plasma influences sperm function and physiology, including sperm motility, the objectives of this study were to characterize the chemistry and protein profiles of Asian elephant seminal plasma and to determine the relationships between seminal plasma components and semen quality. Ejaculates exhibiting good sperm motility (≥65%) expressed higher percentages of spermatozoa with normal morphology (80.3±13.0 vs. 44.9±30.8%) and positive Spermac staining (51.9±14.5 vs. 7.5±14.4%), in addition to higher total volume (135.1±89.6 vs. 88.8±73.1 ml) and lower sperm concentration (473.0±511.2 vs. 1313.8±764.7×106 cells ml−1) compared to ejaculates exhibiting poor sperm motility (≤10%; P<0.05). Comparison of seminal plasma from ejaculates with good versus poor sperm motility revealed significant differences in concentrations of creatine phosphokinase, alanine aminotransferase, phosphorus, sodium, chloride, magnesium, and glucose. These observations suggest seminal plasma influences semen quality in elephants. One- and two-dimensional (2D) gel electrophoresis revealed largely similar compositional profiles of seminal plasma proteins between good and poor motility ejaculates. However, a protein of ∼80 kDa was abundant in 85% of ejaculates with good motility, and was absent in 90% of poor motility ejaculates (P<0.05). We used mass spectrometry to identify this protein as lactotransferrin, and immunoblot analysis to confirm this identification. Together, these findings lay a functional foundation for understanding the contributions of seminal plasma in the regulation of Asian elephant sperm motility, and for improving semen collection and storage in this endangered species.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Lactotransferrin in Asian Elephant (Elephas maximus) Seminal Plasma Correlates with Semen Quality

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Testosterone is the hormone responsible for spermatogenesis and sexual behavior, thus the seasonal pattern of testosterone secretion could limit the male reproductive efficiency during some periods of the year (Todini et al, 2007). Lactate dehydrogenase (LDH) is essential for metabolic processes which provide energy for survival, motility, capacitation and fertility of spermatozoa (Sirat et al, 1996). It has been proposed that the seminal fluid LDH can be used as good indicator of sperm viability (Stamatiads et al, 1984).…”

Section: Introductionmentioning

confidence: 99%

Seasonal Changes in Serum Testosterone, LDH Concentration and Semen Characteristics in Markhoz Goats

Farshad

Yousefi

Moghaddam

et al. 2011

Asian Australas. J. Anim. Sci

View full text Add to dashboard Cite

This experiment was conducted to study variations of serum testosterone and seminal characteristics of Markhoz male goats. Blood samples were obtained via jugular vein, and semen was collected by using an artificial vagina from 14 fertile male goats (2–3 years of age), at 15-day intervals starting on 15 July and ending on 30 October 2010 (during breeding and non-breeding season). Semen volume, total sperm (volume×concentration), live sperm (%), abnormal sperm (%) and semen pH were significantly superior during the late summer and early autumn (breeding season). Variation of sperm density, motility and progressive motility was not significant during the sampling period. The results presented show that the lowest and highest levels of lactate dehydrogenase in the seminal plasma were recorded in late October (2.82 U/ml) and in late August (4.81 U/ml), respectively. Moreover, the study indicated that the serum testosterone concentration was higher during late summer and early autumn (p<0.05) than at any other of sampling period. There were negative correlations between volume and sperm density (−0.135, p<0.05), and positive correlations between volume and percentage live sperm (0.224) and percentage progressive motility (0.194, p<0.01). Sperm density was correlated with live sperm (0.200, p<0.05) and progressive motility (0.202, p<0.01). The correlation between live sperm and progressive motility was 0.554 (p<0.01). Furthermore, the results in this study indicated a significant positive correlation between live sperm and LDH (0.450) and a negative correlation between sperm density and LDH concentration (−0.272) (p<0.01). Significant, but positive correlations were found between sperm motility and LDH (0.542) and testosterone concentration (0.522), respectively (p<0.05). In conclusion, this study demonstrated that the best obtained semen was collected in late summer (during decreasing photoperiod) and early autumn (September and October). This also coincides with the natural breeding season of Markhoz goats in Iran.

show abstract

“…Additionally, APN is involved in many peptide metabolism pathways by catalysing the degradation of peptides related to these pathways (Cheng et al, 2012). Therefore, estimates of these enzymes in seminal plasma have been recommended as markers for semen quality, as they indicate sperm damage (Sirat et al, 1996). Nevertheless, there are a limited number of studies focused on the presence of these enzymes in rabbit semen.…”

Section: Introductionmentioning

confidence: 99%

“…Determinations of biochemical constituents of seminal plasma are needed for semen evaluation, as physical characteristics of semen alone are not completely satisfactory for semen appraisal in current practice (Mann and Lutwak-Mann, 1981). For instance, alkaline phosphatase (ALKP), aspartate transaminase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) are essential for metabolic processes which provide energy for survival, motility and fertility of spermatozoa (Sirat et al, 1996). Likewise, γ-glutamyl transpeptidase (GGT) has been shown to be correlated with sperm motility in horse (Pesch et al, 2006;Dogan et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Determination of enzyme activity in rabbit seminal plasma and its relationship with quality semen parameters

et al. 2015

View full text Add to dashboard Cite

The objective of this study was to determine rabbit seminal plasma enzyme activity. Furthermore, correlations between semen parameters and enzyme activity and male age were examined. The study was performed using 17 New Zealand White males from 5 to 9 mo old. Overall, 252 semen samples were collected from bucks from May to September. Semen characteristics were analysed and the seminal plasma was obtained by centrifugation. The activities of alanyl aminopeptidase (APN), aspartate transaminase (AST), alanine aminotransferase (ALT), γ-glutamyl transpeptidase (GGT), lactate dehydrogenase (LDH) and alkaline phosphatase (ALKP) in the seminal plasma fluid were measured. Significant differences between males were found in APN, GGT, LDH, ALKP and ALT activities (P<0.05). No significant differences between enzyme activity and male age were found. We also observed significant positive correlations between male age and sperm concentration (r=0.26), progressive motility (r=0.17) and amplitude of lateral head displacement (r=0.21), and negative ones between male age and average path velocity (r=-0.56), velocity of the sperm head along its actual curvilinear path (r=-0.61), straight line velocity (r=-0.50), linearity index (r=-0.13), and cytoplasmic droplet (r=-0.33). Furthermore, a significant negative correlation between APN activity and the status of the acrosome (r=-0.20) and significant positive correlations between APN activity and the sperm abnormalities (r=0.21), GGT activity and sperm concentration (r=0.34) and the status of the acrosome (r=0.31), and ALKP activity and sperm concentration were observed (r=0.41). In our study, APN and GGT seem to be the most predictive enzymes for rabbit semen quality.

show abstract

Effect of cryoprotectants on release of various enzymes from buck spermatozoa during freezing

Cited by 25 publications

References 12 publications

Lactotransferrin in Asian Elephant (Elephas maximus) Seminal Plasma Correlates with Semen Quality

Lactotransferrin in Asian Elephant (Elephas maximus) Seminal Plasma Correlates with Semen Quality

Seasonal Changes in Serum Testosterone, LDH Concentration and Semen Characteristics in Markhoz Goats

Determination of enzyme activity in rabbit seminal plasma and its relationship with quality semen parameters

Contact Info

Product

Resources

About