Effect of cryoprotectant-free vitrification versus conventional freezing on human testicular sperm motility: a prospective comparative study

Amer, Medhat; Ismail, Nashaat Nabil; Din, Sameh Fayek GamalEl; Rashad, El-Zahraa; Fakhry, Emad; Hakim, Walaa Abd El; Ragab, Ahmed

doi:10.1080/14647273.2019.1698774

Cited by 1 publication

(1 citation statement)

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“…High-concentration permeable cryoprotectants are cytotoxic and cannot be used for sperm vitrification due to the uniqueness of sperm [ 56 ]. The nonpermeable protectant sucrose can be used for rapid freezing and vitrification of sperm, and vitrification can achieve higher sperm motility [ 57 , 58 ]. At present, studies discussing sperm vitrification are focused on the selection of cryoprotectants and optimization of the cooling process [ 59 ].…”

Section: Application and Advantages Of The Vitrification Technique In...mentioning

confidence: 99%

Advantages of vitrification preservation in assisted reproduction and potential influences on imprinted genes

Chen

Zhang

et al. 2022

Clin Epigenet

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Cryopreservation has important application in assisted reproductive technology (ART). The vitrification technique has been widely used in the cryopreservation of oocytes and embryos, as a large number of clinical results and experimental studies have shown that vitrification can achieve a higher cell survival rate and preimplantation development rate and better pregnancy outcomes. Ovarian tissue vitrification is an alternative method to slow freezing that causes comparatively less damage to the original follicular DNA. At present, sperm preservation mainly adopts slow freezing or rapid freezing (LN2 vapor method), although the vitrification method can achieve higher sperm motility after warming. However, due to the use of high-concentration cryoprotectants and ultra-rapid cooling, vitrification may cause strong stress to gametes, embryos and tissue cells, resulting in potentially adverse effects. Imprinted genes are regulated by epigenetic modifications, including DNA methylation, and show single allele expression. Their accurate regulation and correct expression are very important for the placenta, fetal development and offspring health. Considering that genome imprinting is very sensitive to changes in the external environment, we comprehensively summarized the effect of cryopreservation—especially the vitrification method in ART—on imprinted genes. Animal studies have found that the vitrification of oocytes and embryos can have a significant impact on some imprinted genes and DNA methylation, but the few studies in humans have reported almost no influence, which need to be further explored. This review provides useful information for the safety assessment and further optimization of the current cryopreservation techniques in ART.

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Section: Application and Advantages Of The Vitrification Technique In...mentioning

confidence: 99%

Advantages of vitrification preservation in assisted reproduction and potential influences on imprinted genes

Chen

Zhang

et al. 2022

Clin Epigenet

View full text Add to dashboard Cite

show abstract

Effect of cryoprotectant-free vitrification versus conventional freezing on human testicular sperm motility: a prospective comparative study

Cited by 1 publication

References 25 publications

Advantages of vitrification preservation in assisted reproduction and potential influences on imprinted genes

Advantages of vitrification preservation in assisted reproduction and potential influences on imprinted genes

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