Effect of cryopreservation on seed germination and protocorm development of Vanda tricolor

Jitsopakul, Nipawan; Thammasiri, K.; Yukawa, Tomohisa; Ishikawa, Kinya

doi:10.2306/scienceasia1513-1874.2012.38.244

Cited by 22 publications

(8 citation statements)

References 8 publications

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“…Prior to ultralow temperature preservation, suitable pretreatment methods are used to increase the survival rate of the materials to be preserved. Three pretreatments, namely desiccation, vitrification, and encapsulation-dehydration, are typically applied for orchids [13,[21][22][23][24]. Pretreatment technologies prior to cryopreservation are still a fancy work to investigate now.…”

Section: Cryopreservation Of Orchid Genetic Resources: Seed and Pollenmentioning

confidence: 99%

“…For most orchid species, for example, Bletilla striata, B. formosana, D. candidum (D. moniliforme), and Vanda tricolor [22,35,43,45], preserving tissue immersed directly in LN without any pretreatment results in high mortality rates because of formation of intracellular ice nucleation and growth in the cells [4,20,21,38]. Only a few examples were exceptions, for example, fresh pollens of English walnut (Juglans regia L.) described above [23].…”

Section: Water Contentmentioning

confidence: 99%

“…The vitrification method or the encapsulation-dehydration method may be used for many immature and highly hydrated orchid seeds. The pretreatment in concentrated cryoprotectant solutions before rapid immersion in LN is critical for post-cryopreservation survival of orchid seeds, for example, Bletilla, Cyrtopodium, Doritis, Phaius, Ponerorchis, and Vanda [4,10,11,22,24,41,43,52].…”

Section: Maturitymentioning

confidence: 99%

See 2 more Smart Citations

Cryopreservation of Orchid Genetic Resources by Desiccation: A Case Study of Bletilla formosana

RungYi¹,

Chang²,

Hsieh³

et al. 2016

Cryopreservation in Eukaryotes

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Many native orchid populations declined yearly due to economic development and climate change. This resulted in some wild orchids being threatened. In order to maintain the orchid genetic resources, development of proper methods for the long-term preservation is urgent. Low temperature or dry storage methods for the preservation of orchid genetic resources have been implemented but are not effective in maintaining high viability of certain orchids for long periods. Cryopreservation is one of the most acceptable methods for long-term conservation of plant germplasm. Orchid seeds and pollens are ideal materials for long-term preservation (seed banking) in liquid nitrogen (LN) as the seeds and pollens are minute, enabling the storage of many hundreds of thousands of seeds or pollens in a small vial, and as most species germinate readily, making the technique very economical. This article describes cryopreservation of orchid genetic resources by desiccation and a case study of Bletilla formosana. We hope to provide a more practical potential cryopreservation method for future research needs.

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Section: Cryopreservation Of Orchid Genetic Resources: Seed and Pollenmentioning

confidence: 99%

Section: Water Contentmentioning

confidence: 99%

Section: Maturitymentioning

confidence: 99%

See 1 more Smart Citation

Cryopreservation of Orchid Genetic Resources by Desiccation: A Case Study of Bletilla formosana

RungYi¹,

Chang²,

Hsieh³

et al. 2016

Cryopreservation in Eukaryotes

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“…Seed and protocorm cryopreservation utilises a range of different available methods including: direct freezing, encapsulation-vitrification and droplet-freezing, each tested in several taxa (Sakai and Engelmann 2007 ; Sopalun et al 2010 ; Jitsopakul et al 2012 ; Hu et al 2013 ; Galdiano et al 2014 ; Teixeira da Silva et al 2014 ; Popova et al 2016 ). However, protocorms, a widely used propagule for cryopreservation, are prone to cryopreservation injury due to their cellular structure and high-water content.…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation without vitrification suitable for large scale cryopreservation of orchid seeds

2018

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BackgroundOrchids are under threat from human activities and climate change, with populations limited to small geographic hotspots. This makes them ideal candidates for ex situ conservation. Orchid seeds are desiccation tolerant, but often have poor longevity in seed banks and cryopreservation of orchid protocorms is complex and expensive. Therefore, simple methods for large-scale storage programs are essential to store orchid seeds of different life forms. Seeds of five species representing epiphytic, lithophytic and terrestrial orchids from the Central Highlands of Madagascar were studied to find a simple and effective system of cryopreservation. The use of a vitrification solution prior to cryopreservation to improve survival was investigated, as well as the use of symbiotic and asymbiotic germination media to maximise germination after cryopreservation. Using the filter paper packet method, dried seeds were stored in vapour phase above liquid nitrogen and recovered after thawing with both symbiotic and asymbiotic media.ResultsThe study revealed that cryoprotection is not essential for the species in this study, which represented a range of lifeforms. Vitrification generally led to a decrease in germination post cryopreservation. The use of a symbiotic germination medium post cryopreservation was found to be successful in the species in which it was tested. However, the use of an asymbiotic medium was successful for all the species in this study.ConclusionsVitrification was not essential for the species in this study as the orchid seeds were already ultralow temperature and desiccation tolerant. However, further studies using more species are required to validate this approach. This may be an ecophysiological or genetic trait of these species. Therefore, this form of dry seed cryopreservation could form part of ex situ orchid seed conservation using a standard method. The methods developed here will store greater genetic diversity compared to what can be achieved with protocorms and are suitable for both asymbiotic and symbiotic recovery after cryopreservation. This will help reduce the time and cost of ex situ conservation, and help develop universal protocols for large genera, compared to custom protocols required for protocorm cryopreservation.Electronic supplementary materialThe online version of this article (10.1186/s40529-018-0229-7) contains supplementary material, which is available to authorized users.

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“…Vitrification is one the protection methods against ultracold temperatures in which the formation of ice crystals is prevented without significant decrease in intracellular water (Panis and Lambardi, 2005;Jitsopakul, 2012). In the cooling process, glycerol is used as a preservative against freezing.…”

Section: Introductionmentioning

confidence: 99%

Investigation on possibility of cryopreservation of Satureja rechingeri seeds

2014

Int. J. Biosci.

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Satureja rechingeri, belonging to the Lamiaceae family, is one of the native range species of Iran. Considering the loss of Satureja rechingeri germplasm, due to the limited distribution, biotic and abiotic stresses, overgrazing of livestock, and generally the risks threatening the habitat of this species, fundamental actions need to be performed towards the preservation of Satureja rechingeri genetic diversity. Cryopreservation has the ability to preserve the seeds for a long time with much lower costs than other methods and without loss of seed viability. In the current study, treatments of vitrification, glycerol (30%), and the reduction of seed moisture before entering the liquid nitrogen were used. After one week, the treated seeds were removed from liquid nitrogen and were melted in sterile distilled water bath with a temperature of 42 °C. Then, the seeds were washed by sucrose solution (1.2 M) using shaker in three stages and were cultured with control seeds on sterile wet filter papers and transferred to a growth chamber. According to the obtained results of seed cryopreservation of Satureja rechingeri, no significant difference was found for all germination and seed growth characteristics except germination percentage and rate. Our results clearly indicate the effectiveness of this method for the seed preservation of Satureja rechingeri as a unique and endangered species for a very long time.

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Effect of cryopreservation on seed germination and protocorm development of Vanda tricolor

Cited by 22 publications

References 8 publications

Cryopreservation of Orchid Genetic Resources by Desiccation: A Case Study of Bletilla formosana

Cryopreservation of Orchid Genetic Resources by Desiccation: A Case Study of Bletilla formosana

Cryopreservation without vitrification suitable for large scale cryopreservation of orchid seeds

Investigation on possibility of cryopreservation of Satureja rechingeri seeds

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