Effect of cryopreservation and transplantation on the expression of kit ligand and anti-Mullerian hormone in human ovarian tissue

David, Allan E.; Langendonckt, Anne Van; Gilliaux, Sébastien; Dolmans, Marie-Madeleine; Donnez, Jacques; Amorim, Christiani Andrade

doi:10.1093/humrep/des013

Cited by 53 publications

(36 citation statements)

References 41 publications

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“…2f and 3), demonstrating that follicles are functional after long-term autografting. These data are consistent with previous results showing ungrafted fresh tissue to contain around 60 % of follicles positive for AMH, increasing to around 80 % after slow-freezing of human ovarian tissue grafted to mice [40].…”

Section: Discussionsupporting

confidence: 93%

Impact of the cryopreservation technique and vascular bed on ovarian tissue transplantation in cynomolgus monkeys

Dolmans

Binda

Jacobs

et al. 2015

J Assist Reprod Genet

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Purpose The aim of this study was to determine the best combination in terms of cryopreservation techniques and vascular bed preparation before grafting in order to obtain functional ovarian tissue after transplantation. Methods Five cynomolgus monkeys were used. Strips from 10 ovaries were cryopreserved, 5 by vitrification (V), and 5 by slow-freezing (SF). Pieces of fresh ovarian tissue were used for controls. After 1 month, the strips were autografted to two different vascular beds, healed (HB) or freshly decorticated (FDB), constituting four study groups: SF-HB, SF-FDB, V-HB, and V-FDB. These were compared to fresh tissue. After 6 months, the ovaries were removed and several parameters analyzed: follicle quality, stage, density, proliferation, apoptosis, functionality, vascularization, and fibrosis. Mixed effect linear regression models were built to assess the impact of cryopreservation and vascular bed preparation on ovarian tissue viability and functionality. p values were adjusted for multiple testing using the Benjamini-Hochberg method, and q values<0.20 were considered significant in order to achieve a 20 % false discovery rate. Results Compared to fresh tissue, no difference was observed in the percentage of morphologically normal follicles, while a significant increase was noted in the follicle proliferation rate (41 %, q=0.19), percentage of antral follicles (12 %, q=0.14), and number of vessels per area (3.3 times, q=0.07) in the V-FDB group. Conclusions Vitrification associated with FDB vascular bed preparation is the best combination to obtain functional autografted ovarian tissue. Further studies are nevertheless required, with confirmed pregnancies and live births before introducing the procedure into clinical practice.Capsule Vitrification associated with freshly decorticated vascular bed preparation appears to be the best combination to obtain functional autografted ovarian tissue. M. M. Dolmans and M. M. Binda contributed equally to this work.Electronic supplementary material The online version of this article

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Section: Discussionsupporting

confidence: 93%

Impact of the cryopreservation technique and vascular bed on ovarian tissue transplantation in cynomolgus monkeys

Dolmans

Binda

Jacobs

et al. 2015

J Assist Reprod Genet

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“…It is also important to recognize that many studies report the effects of vitrification shortly, if not immediately, after thawing the cryopreserved tissue. While there is overwhelming evidence that grafting ovarian tissue following warming leads to activation of the primordial follicle reserve due to ischemiareperfusion [30,35,36], there is contradictory evidence as to whether cryoprotectant exposure and/or vitrification of ovarian tissue induces activation of primordial follicles. One study found that the type of cryoprotectant used affects the results, with propanediol increasing the activation rate following grafting more than with DMSO [37].…”

Section: Discussionmentioning

confidence: 99%

Damage to fetal bovine ovarian tissue caused by cryoprotectant exposure and vitrification is mitigated during tissue culture

Mouttham

Fortune

Comizzoli

2015

J Assist Reprod Genet

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Purpose The objective of this study is to characterize the impact of exposure to cryoprotectants followed by vitrification on primordial follicle survival and activation using a fetal bovine model. Methods In the first study, fetal bovine cortical pieces were exposed to cryoprotectants with or without sucrose and cultured up to 7 days in the presence or absence of insulin. In the second study, cortical pieces were exposed to cryoprotectants with or without sucrose, vitrified, and cultured up to 7 days after warming in the presence or absence of insulin. Viability and morphology of follicles, as well as proliferation and/or DNA repair in ovarian tissue were analyzed.Results When compared to non-exposed controls, normal follicular morphology was affected in groups exposed to cryoprotectants only immediately post-exposure and after 1 day of culture, but improved by day 3 and did not significantly differ by day 7. Similarly, normal follicular morphology was compromised in vitrified groups after warming and on day 1 compared to controls, but improved by days 3 and 7. Proliferation and/or DNA repair in ovarian tissue was not affected by vitrification in this model. Cryoprotectant exposure and vitrification of ovarian tissue did not impair the activation of primordial follicles in response to insulin, although activation was delayed relative to non-exposed controls. Interestingly, sucrose had no noticeable protective effect. Conclusion Vitrified fetal bovine ovarian tissue has the intrinsic capacity to mitigate the immediate damage to primordial follicles' morphology and retains the capacity to activate. These findings provide a basis for a successful cryopreservation protocol for ovarian cortical tissue in other species including humans.

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“…45 The number of primordial follicles that enter the growing pool is not likely to be influenced by the high levels of FSH for the 14 days after transplantation as receptors for this ligand are not present. 27 Local peptide hormones such as Kit Ligand or anti-Mullerian hormone have also been implicated in follicle activation after transplantation 46 and could be supplemented through peptide delivery from the biomaterial. Moreover, systems biology analysis of follicles across various stages of development are identifying key factors.…”

Section: Fig 6 Graft Function (A)mentioning

confidence: 99%

Fibrin-Mediated Delivery of an Ovarian Follicle Pool in a Mouse Model of Infertility

Smith

Shikanov

Kniazeva

et al. 2014

Tissue Engineering Part A

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Effect of cryopreservation and transplantation on the expression of kit ligand and anti-Mullerian hormone in human ovarian tissue

Cited by 53 publications

References 41 publications

Impact of the cryopreservation technique and vascular bed on ovarian tissue transplantation in cynomolgus monkeys

Impact of the cryopreservation technique and vascular bed on ovarian tissue transplantation in cynomolgus monkeys

Damage to fetal bovine ovarian tissue caused by cryoprotectant exposure and vitrification is mitigated during tissue culture

Fibrin-Mediated Delivery of an Ovarian Follicle Pool in a Mouse Model of Infertility

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