Effect of crosslinking on the elasticity of polyelectrolyte multilayer films measured by colloidal probe AFM

Francius, Grégory; Hemmerlé, Joseph; Ohayon, Jacques; Schaaf, Pierre; Voegel, J.‐C.; Picart, Catherine; Senger, Bernard

doi:10.1002/jemt.20275

Cited by 91 publications

(131 citation statements)

References 38 publications

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“…5). [134] Using a different strategy, Li and Haynie [135] investigated cross-linking and stabilization of polypeptide PEMs by formation of disulfide bonds, which are reversible. Disulfide bonds are involved in the structural stabilization of proteins.…”

Section: Chemical Cross-linkingmentioning

confidence: 99%

Multiple Functionalities of Polyelectrolyte Multilayer Films: New Biomedical Applications

Boudou¹,

Crouzier²,

Ren³

et al. 2010

Advanced Materials

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686

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The design of advanced functional materials with nanometer- and micrometer-scale control over their properties is of considerable interest for both fundamental and applied studies because of the many potential applications for these materials in the fields of biomedical materials, tissue engineering, and regenerative medicine. The layer-by-layer deposition technique introduced in the early 1990s by Decher, Moehwald, and Lvov is a versatile technique, which has attracted an increasing number of researchers in recent years due to its wide range of advantages for biomedical applications: ease of preparation under "mild" conditions compatible with physiological media, capability of incorporating bioactive molecules, extra-cellular matrix components and biopolymers in the films, tunable mechanical properties, and spatio-temporal control over film organization. The last few years have seen a significant increase in reports exploring the possibilities offered by diffusing molecules into films to control their internal structures or design "reservoirs," as well as control their mechanical properties. Such properties, associated with the chemical properties of films, are particularly important for designing biomedical devices that contain bioactive molecules. In this review, we highlight recent work on designing and controlling film properties at the nanometer and micrometer scales with a view to developing new biomaterial coatings, tissue engineered constructs that could mimic in vivo cellular microenvironments, and stem cell "niches."

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Section: Chemical Cross-linkingmentioning

confidence: 99%

Multiple Functionalities of Polyelectrolyte Multilayer Films: New Biomedical Applications

Boudou¹,

Crouzier²,

Ren³

et al. 2010

Advanced Materials

Self Cite

686

702

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“…Table 1 Summary of the physico-chemical properties of cross-linked (PLL/HA) 12 films for EDC varied between 5 and 100 mg/mL (EDCx means that EDC final concentration is x mg/mL). Young's modulus was determined by AFM nano-indentation experiments [31] , film roughness was determined from AFM images of film topography. Serum adsorption was measured by quartz crystal microbalance.…”

Section: Supplementary Materialsmentioning

confidence: 99%

Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation

Ren

Crouzier

Roy

et al. 2008

Adv Funct Materials

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Beside chemical properties and topographical features, mechanical properties of gels have been recently demonstrated to play an important role in various cellular processes, including cell attachment, proliferation, and differentiation. In this work, we used multilayer films made of poly (L-lysine)/Hyaluronan (PLL/HA) of controlled stiffness to investigate the effects of mechanical properties of thin films on skeletal muscle cells (C2C12 cells) differentiation. Prior to differentiation, cells need to adhere and proliferate in growth medium. Stiff films (E 0 > 320 kPa) promoted formation of focal adhesions and organization of the cytoskeleton as well as an enhanced proliferation, whereas soft films were not favorable for cell anchoring, spreading or proliferation. Then C2C12 cells were switched to a low serum containing medium to induce cell differentiation, which was also greatly dependent on film stiffness. Although myogenin and troponin T expressions were only moderately affected by film stiffness, the morphology of the myotubes exhibited striking stiffness-dependent differences. Soft films allowed differentiation only for few days and the myotubes were very short and thick. Cell clumping followed by aggregates detachment could be observed after ~2 to 4 days. On stiffer films, significantly more elongated and thinner myotubes were observed for up to ~ 2 weeks. Myotube striation was also observed but only for the stiffer films. These results demonstrate that film stiffness modulates deeply adhesion, proliferation and differentiation, each of these processes having its own stiffness requirement.

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“…1Ab that the apparent moduli are roughly 50, 200 and 500 kPa for the architectures (PLL/HA) 24 -(PSS/PAH) n with n2, 5, 12, respectively. It is known from earlier investigations (Francius et al, 2006) that the elastic modulus of the native (PLL/HA) 24 architecture is under 1 kPa and is considered here as our zero. Henceforth, we shall use the short-hand notations E0, E50, E200 and E500 for the (PLL/HA) 24 , (PLL/HA) 24 -(PSS/PAH) n films with n2, 5, 12, respectively, and 0/2, 0/5 and 0/12 for (PSS/PAH) 2 , (PSS/PAH) 5 and (PSS/PAH) 12 films, respectively (see Table 1).…”

Section: Progressive Stiffening Of the Pem Films Increases Cell Adhesionmentioning

confidence: 99%

Selective and uncoupled role of substrate elasticity in the regulation of replication and transcription in epithelial cells

Kocgozlu

Lavalle

Koenig

et al. 2010

Journal of Cell Science

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SummaryActin cytoskeleton forms a physical connection between the extracellular matrix, adhesion complexes and nuclear architecture. Because tissue stiffness plays key roles in adhesion and cytoskeletal organization, an important open question concerns the influence of substrate elasticity on replication and transcription. To answer this major question, polyelectrolyte multilayer films were used as substrate models with apparent elastic moduli ranging from 0 to 500 kPa. The sequential relationship between Rac1, vinculin adhesion assembly, and replication becomes efficient at above 200 kPa because activation of Rac1 leads to vinculin assembly, actin fiber formation and, subsequently, to initiation of replication. An optimal window of elasticity (200 kPa) is required for activation of focal adhesion kinase through auto-phosphorylation of tyrosine 397. Transcription, including nuclear recruitment of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1), occurred above 50 kPa. Actin fiber and focal adhesion signaling are not required for transcription. Above 50 kPa, transcription was correlated with v-integrin engagement together with histone H3 hyperacetylation and chromatin decondensation, allowing little cell spreading. By contrast, soft substrate (below 50 kPa) promoted morphological changes characteristic of apoptosis, including cell rounding, nucleus condensation, loss of focal adhesions and exposure of phosphatidylserine at the outer cell surface. On the basis of our data, we propose a selective and uncoupled contribution from the substrate elasticity to the regulation of replication and transcription activities for an epithelial cell model.

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Effect of crosslinking on the elasticity of polyelectrolyte multilayer films measured by colloidal probe AFM

Cited by 91 publications

References 38 publications

Multiple Functionalities of Polyelectrolyte Multilayer Films: New Biomedical Applications

Multiple Functionalities of Polyelectrolyte Multilayer Films: New Biomedical Applications

Polyelectrolyte Multilayer Films of Controlled Stiffness Modulate Myoblast Cell Differentiation

Selective and uncoupled role of substrate elasticity in the regulation of replication and transcription in epithelial cells

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