Effect of cow age on the in vitro developmental competence of oocytes obtained after FSH stimulation and coasting treatments

Landry, David; Bellefleur, Anne‐Marie; Labrecque, Rémi; Grand, François‐Xavier; Vigneault, Christian; Blondin, Patrick; Sirard, Marc‐André

doi:10.1016/j.theriogenology.2016.04.064

Cited by 66 publications

(58 citation statements)

References 28 publications

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“…As expected, no differences were observed in terms of IVM, but both cleavage and blastocyst absolute rates and kinetics differed between the two groups of oocytes (Tables and ), with prepubertal embryos achieving lower rates in a longer time. Such observations are in agreement with previous results obtained in several species (Landry et al, ; Leoni et al, ) and confirmed the lower potential of gametes derived from prepubertal donors.…”

Section: Discussionsupporting

confidence: 94%

“…), with prepubertal embryos achieving lower rates in a longer time. Such observations are in agreement with previous results obtained in several species(Landry et al, 2016; Leoni et al, 2006) and confirmed the lower potential of gametes derived from prepubertal donors.The temporal expression analysis of the panel of genes showed specific patterns in oocytes and embryos derived from adult and prepubertal donors(Figures 1a-5a). The late EGA that characterizes large domestic animals compared to rodents (8-16 cell stage vs. 1-2 cell stage) allowed to detail the expression patterns from fertilization to EGA, identifying the variations specific to the different developmental stages.With a few gene-specific exceptions, analysis during maturation and early development in the adults evidenced some common features.…”

supporting

confidence: 93%

“…The efforts in identifying the reasons for the reduced developmental competence not only improved the rates of embryo production from prepubertal donors (Earl, Irvine, Kelly, Rowe, & Armstrong, ; Ledda et al, ), but interestingly shed light into numerous aspects involved in oocyte potential. Morphological, cellular, biochemical and molecular studies confirmed the differential competence of gametes derived from adult and prepubertal donors in terms of resistance to cryopreservation (Leoni et al, ), metabolism and ultrastructure (O'Brien, Dwarte, Ryan, Maxwell, & Evans, ), transcript abundance (Bebbere et al, ; Ledda et al, ; Leoni et al, ; Romar et al, ), in vitro developmental competence and kinetics (Landry et al, ; Leoni et al, , ; Majerus et al, ), mitochondrial distribution (Leoni et al, ) and methylation dynamics (Masala et al, ). Although the differential model has become a useful research tool independently on its breeding application, the limited competence of prepubertal oocytes is yet not fully understood.…”

Section: Introductionmentioning

confidence: 89%

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Delay in maternal transcript degradation in ovine embryos derived from low competence oocytes

Masala

Ariu

Bogliolo

et al. 2018

Molecular Reproduction Devel

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Oocytes from prepubertal animals have a reduced ability to undergo embryo development and produce viable offspring. The present work used an ovine model consisting of oocytes derived from adult and prepubertal donors to assess the molecular status of oocytes and preimplantation embryos with different developmental competence. The lower potential of oocytes of young donors was confirmed in terms of in vitro developmental capabilities and kinetics. A panel of genes including maternal effect (DPPA3, GDF9, NMP2, ZAR1) and housekeeping genes (ACTB, RPL19, SDHA, YWHAZ, ATP1A1), genes involved in DNA methylation (DNMT1, DNMT3A, DNMT3B), genomic imprinting (IGF2R), pluripotency (NANOG, POU5F1) and cell cycle regulation (CCNB1, CDK1, MELK) was relatively quantified. Temporal analysis during oocyte maturation and preimplantation embryo development evidenced patterns associated with donor age. With a few gene-specific exceptions, the differential model showed a reduced transcript abundance in immature prepubertal oocytes that completely reversed trend after fertilization, when higher mRNA levels were consistently observed in early embryos, indicating a delay in maternal transcript degradation. We propose that the molecular shortage in the prepubertal oocyte may affect its developmental potential and impair the early pathways of maternal mRNA clearance in the embryo. While confirming the different potential of oocytes derived from adult and prepubertal donors, our work showed for the first time a consistent delay in maternal transcript degradation in embryos derived from low competence oocytes that interestingly recalls the delayed developmental kinetics. Such abnormal transcript persistence may hinder further development and represents a novel perspective on the complexity of developmental competence.

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Section: Discussionsupporting

confidence: 94%

supporting

confidence: 93%

Section: Introductionmentioning

confidence: 89%

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Delay in maternal transcript degradation in ovine embryos derived from low competence oocytes

Masala

Ariu

Bogliolo

et al. 2018

Molecular Reproduction Devel

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“…Step 3: In vitro embryo production Previously described standard bovine operating procedures and media were used in our studies for IVM/F/C to the blastocyst stage, in Holstein cattle and Mediterranean buffalo (Landry et al, 2016). In general, this seems to be the common procedure from what is found in the literature.…”

Section: Introductionmentioning

confidence: 99%

Laparoscopic ovum pick-up for in vitro embryo production from dairy bovine and buffalo calves

Baldassarre

Bordignon

2018

Anim Reprod

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Laparoscopic ovum pick-up (LOPU) conducted on bovine and buffalo calves of 2-6-month of age, followed by in vitro embryo production and transfer into synchronous adult recipients, is a powerful tool for accelerated genetic gain and early dissemination of top genetics. In its current state, the technology is characterized by higher oocyte recovery rates, lower oocyte-to-embryo yields, and similar pregnancy and term development rates compared with adult counterparts. Improvements in oocyte competence have been made in recent years mainly through gonadotropin stimulation protocols tailored for prepubertal donors. These advances have brought the technology to the point of been apt for commercial application. However, future research must focus on increasing the proportion of fully competent oocytes recovered from calves thereby further empowering the role this technology platform can play in programs for accelerated dissemination of superior genetics.

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“…In vitro procedures were adapted from Landry et al (). The COCs from each animal were placed in hepes‐buffered thyrode’s‐lactate (TLH) solution (supplemented with 0.3% bovine serum albumin, 0.2 mM pyruvate, and 50 mg/ml gentamicin) and washed three times to separate them from the follicular fluid.…”

Section: Methodsmentioning

confidence: 99%

Granulosa cells of prepubertal cattle respond to gonadotropin signaling and upregulate genes that promote follicular growth and prevent cell apoptosis

Michalovic

Currin

Gutierrez

et al. 2018

Molecular Reproduction Devel

Self Cite

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Oocytes collected from prepubertal animals are known to be less developmentally competent than those from adult animals. There is evidence suggesting that acquisition of developmental competence in bovine oocytes may be linked to the expression profile of genes in the granulosa cells (GCs). Cumulus–oocyte complexes (COC) and GCs were collected from 12 Holstein heifers between 2 and 6 months of age (nine follicle‐stimulating hormone [FSH] treated and three untreated) and eight FSH‐treated cows. The COCs from prepubertal animals were matured, fertilized, and cultured in vitro to assess development to the blastocyst stage. The relative messenger RNA (mRNA) abundance of FSHR, StAR, CYP19A1, HSD3B1, CX43, FOXO1, and XIAP in GCs were quantified by real‐time quantitative polymerase chain reaction. Results from this study revealed that GCs of prepubertal animals respond to FSH treatment by increasing mRNA levels of genes promoting estradiol synthesis and follicular growth ( FSHR and CYP19A1), and preventing cell apoptosis ( XIAP), and by decreasing mRNA levels of genes promoting progesterone production ( StAR and HSD3B1). This study also revealed that the relative mRNA abundance of FOXO1 in GCs is associated with oocyte competence to support embryo development to the blastocyst stage in prepubertal Holstein heifers.

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Effect of cow age on the in vitro developmental competence of oocytes obtained after FSH stimulation and coasting treatments

Cited by 66 publications

References 28 publications

Delay in maternal transcript degradation in ovine embryos derived from low competence oocytes

Delay in maternal transcript degradation in ovine embryos derived from low competence oocytes

Laparoscopic ovum pick-up for in vitro embryo production from dairy bovine and buffalo calves

Granulosa cells of prepubertal cattle respond to gonadotropin signaling and upregulate genes that promote follicular growth and prevent cell apoptosis

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