Effect of cosubstrates on primary biodegradation of triphenylmethane dyes by &lt;i&gt; Pseudomonas&lt;/i&gt; sp.

Oranusi, NA; Ogugbue, Chimezie Jason

doi:10.4314/ajazeb.v7i1.41144

Cited by 6 publications

(8 citation statements)

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“…Although some strains of the genus of Pseudomonas, including Pseudomonas pseudomallei 13NA, Pseudomonas spp., Pseudomonas mendocina MCM B-402, Pseudomonas aeruginosa, Pseudomonas sp. and Pseudomonas putida, have been reported to decolorize triphenylmethane dyes (Yatome et al 1981;Sarnaik and Kanekar 1995;Sarnaik and Kanekar 1999;Lin et al 2004;Oranusi and Ogugbue 2005;Chen et al 2007), only Pseudomonas aeruginosa has been shown to decolorize MG. However, its decolorizing mechanism has not yet been elucidated (Lin et al 2004).…”

Section: Discussionmentioning

confidence: 96%

Isolation of a malachite green-degrading Pseudomonas sp. MDB-1 strain and cloning of the tmr2 gene

Hong

Yan

et al. 2009

Biodegradation

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The release of malachite green, a commonly used triphenylmethane dye, into the environment is causing increasing concern due to its toxicity, mutagenicity, and carcinogenicity. A bacterial strain that could degrade malachite green was isolated from the water of an aquatic hatchery. It was identified as a Pseudomonas sp. based on the morphological, physiological, and biochemical characteristics, as well as the analysis of 16S rRNA gene sequence and designated as MDB-1. This strain was capable of degrading both malachite green and leucomalachite green, as well as other triphenylmethane dyes including Crystal Violet and Basic Fuchsin. The gene tmr2, encoding the triphenylmethane reductase from MDB-1, was cloned, sequenced and effectively expressed in E. coli. These results highlight the potential of this bacterium for the bioremediation of aquatic environments contaminated by malachite green.

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Section: Discussionmentioning

confidence: 96%

Isolation of a malachite green-degrading Pseudomonas sp. MDB-1 strain and cloning of the tmr2 gene

Hong

Yan

et al. 2009

Biodegradation

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“…The enhancement of decolourisation of dyes as a result of metabolism of glucose, soluble starch and sucrose which generated external electron donors was reported by Padmavathy et al, (2003) and Oranusi and Ogugbue (2005).…”

Section: Resultsmentioning

confidence: 89%

Biotransformation of Food Dyes by Human Intestinal Bacteria (Streptococcus faecalis, Eschericia coli)

Oranusi¹,

Njoku²

2009

Journal of Applied Sciences and Environmental Management

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Biotransformation of food dyes (Tartrazine and Quinoline yellow) by Streptococcus faecalis and Escherichia coli isolated from human intestinal microflora was investigated. Decolourisation of the media containing the dyes was used as an index of biotransformation. Biotransformation was higher under aerobic than under anaerobic conditions. The results obtained were attributed to the organisms cytosolic flavin-dependent reductases and redox equivalents generated by metabolism of soluble starch which transfer electrons to the chromophoric group of the dyes. The potential health risk of the resulting colourless metabolites (aromatic amines) is under investigation. @JASEM

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“…The choice for study the Evans blue and brilliant green was firstly dictated by the fact that they are representatives of two most popular classes of applied dyes, secondly due to the common use exactly these dyes we can expect their presence in the wastewaters and then in aquatic ecosystems. The triphenylmethane brilliant green (classified as hazardous substance), is used at the textile industry for dying the silk, wool, cotton, leather and polymers, in laboratories for preparation staining, because of the antiseptic properties, at the past was used in medicine (Oranusi and Ogugbue 2005;Rehman et al 2015). The disazo Evans blue (classified as nontoxic substance), is commonly used in medicine for estimation the blood and plasma volume, the permeability of the blood-brain barrier to macromolecules, more over at diagnostics of damages of backbone and muscles and for preparation staining and others.…”

Section: Introductionmentioning

confidence: 99%

Fed-Batch Decolourization of Mixture of Brilliant Green and Evans Blue by Bacteria Species Applied as Pure and Mixed Cultures: Influence of Growth Conditions

Zabłocka-Godlewska

Przystaś

2020

Water Air Soil Pollut

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Synthetic dyes, generally resistant, toxic and carcinogenic presents a substantial risk to the environment and health of human. The present study was aimed to decolourize a dye mixture (Evans blue and brilliant green) by selected bacterial strains cultivated at different growth conditions (e.g. unmodified, correction of pH value and supplementation with nutrients). The bacterial strains used as pure and mixed cultures include facultative anaerobes Aeromonas hydrophila (Abs37), Citrobacter sp. (Cbs50) and obligatory aerobe Pseudomonas putida (Pzr3). The efficiency of removal of all successive doses of dye mixture (4-5 doses, total load 170-200 mg/l) was tested in static conditions in fedbatch bioreactors. The modification of bacteria growth conditions influenced on decolourization efficiency: most advantageous was pH value correction combined with nutrient supplementation then pH correction alone and nutrient supplementation (final removal results 95.6-100%, 92.9-100% and 89.1-97.2%, respectively). The mixed bacterial cultures removed the total load of dyes with higher efficiency than pure strains (final removal 95.2-100% and 84.0-98.2%, respectively). The best results were obtained for the mixture of facultative anaerobe Citrobacter sp. and obligatory aerobe Pseudomonas putida which removed the highest load of dye mixture (200 mg/l introduced at five doses) in the shortest time (288 h), while the others pure and mixed cultures needed 425-529 h for removal four doses of dye mixture (total load 170 mg/l). The zoo-and phytotoxicity decreased after these processes (from V class of toxicity (extremely toxic) even to II class (low toxicity)). The main mechanisms of decolourization was biotransformation/biodegradation, supported by sorption.

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Effect of cosubstrates on primary biodegradation of triphenylmethane dyes by <i> Pseudomonas</i> sp.

Cited by 6 publications

References 0 publications

Isolation of a malachite green-degrading Pseudomonas sp. MDB-1 strain and cloning of the tmr2 gene

Isolation of a malachite green-degrading Pseudomonas sp. MDB-1 strain and cloning of the tmr2 gene

Biotransformation of Food Dyes by Human Intestinal Bacteria (Streptococcus faecalis, Eschericia coli)

Fed-Batch Decolourization of Mixture of Brilliant Green and Evans Blue by Bacteria Species Applied as Pure and Mixed Cultures: Influence of Growth Conditions

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