Effect of Cortisone upon Chondroitin Sulfate Synthesis by Animal Tissues.

Layton, Laurence L.

doi:10.3181/00379727-76-18571

Cited by 124 publications

(21 citation statements)

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“…This concept is supported by the studies of Martin, Chaudhuri, Green, and McKinney (13), Huisman (14), and Layton (15), who have described specific alterations in metabolic processes of tissues exposed in vitro to cortisone. The present studies suggest that some metabolic changes do not depend on influences other than the hormone itself, because essentially the same effect observed in leukocytes from patients receiving ACIH or cortisone therapy could be induced in normal leukocytes by exposure of the cells to cortisone or hydrocortisone in vitro.…”

supporting

confidence: 60%

“…The conflicting observations in vivo and in vitro suggest that cellular alteration may take place in vivo, perhaps by indirect means, but that this alteration may persist in vitro, once established, at least in embryonic tissues. However, there are other indications (as in the work of Martin, Chaudhuri, Green, and McKinney [13], possibly Huisman [14], and Layton [15] ) that alteration of cellular metabolism can be induced by the action of cortisone, entirely in vitro.…”

mentioning

confidence: 99%

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The Effect of Hydrocortisone and of Piromen in Vitro on Leukocytes of Patients Receiving Acth and Cortisone Therapy 1

Kerby¹,

Barrett²

1954

J. Clin. Invest.

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supporting

confidence: 60%

mentioning

confidence: 99%

The Effect of Hydrocortisone and of Piromen in Vitro on Leukocytes of Patients Receiving Acth and Cortisone Therapy 1

Kerby¹,

Barrett²

1954

J. Clin. Invest.

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“…of cortisone on connective ti.sue is indicated. Layton (1951) said that high doses of cortisone appeared to inhibit the synthesis of chondroitin sulfate from the tissues. Be said this effect may be secondary to the alteration in protein metabolism indicated by a large negative sulfur balance.…”

Section: Cortisone Actionmentioning

confidence: 99%

Labeling Index and Cellular Density in Palatine Shelves of Cleft-Palate Mice

1969

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“…Numerous reports have indicated that systemically administered corticosteroids may alter cell metabolism and, specifically, can cause inhibition of protein and polysaccharide synthesis in a number of organs (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19). Little is known, however, regarding the effect of the hormone(s) on articular cartilage; but, from the data reported for other tissues, as well as theoretic considerations, it would seem likely that systemically administered steroids would significantly interfere with the anabolic activities of the cartilage cell.…”

mentioning

confidence: 99%

The effect of systemic corticosteroids on rabbit articular cartilage

Mankin¹,

Zarins²,

Jaffe³

1972

Arthritis & Rheumatism

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Rabbits were treated for up to 9 weeks with daily doses of cortisone. Cartilage from the distal femur was studied biochemically at regular intervals, and no change in the content of DNA but a significant and progressive decline in the concentration of hexosamine were noted. In vitro incorporation of J5S04 and W-glycine was depressed markedly. Because the test animals lost 25% of their body weight, a separate group of starved controls were studied and the changes noted were similar to those seen with corticoid treatment but were not as marked.Prior studies from this and other laboratories demonstrated the potential deleterious effects of intraarticularly administered corticosteroids on articular cartilage of experimental animals. It has been shown that, when injected into the animal joint, both soluble and slightly soluble steroid products used in clinical treatment may significantly inhibit protein and polysaccharide synthesis by the chondrocyte, and that prolonged treatment may produce specific pathologic lesions (1 -5). Submitted for publication April 19, 1972; accepted July 13, 1972. Numerous reports have indicated that systemically administered corticosteroids may alter cell metabolism and, specifically, can cause inhibition of protein and polysaccharide synthesis in a number of organs (5-19). Little is known, however, regarding the effect of the hormone(s) on articular cartilage; but, from the data reported for other tissues, as well as theoretic considerations, it would seem likely that systemically administered steroids would significantly interfere with the anabolic activities of the cartilage cell.The purpose of this report is to describe the results of an experimental study in which cortisone acetate, 4.5 mg/kg/day, was administered intramuscularly to adult rabbits. T h e articular cartilage from the distal femur was analyzed for alterations in biochemical constituents and rates of synthesis of the matrix at intervals up to 9 weeks. Early analyses of the data demonstrated a significant effect on the general metabolic state of the rabbit, as indicated by a weight loss of up to 28.8%. These findings dictated a second metabolic experiment, in which another group of rabbits was partially starved over a 7-week period. T h e articular cartilage from both groups of animals was studied biochemically and metabolically, and the data showed a significant depletion in polysaccharide content and a decrease in rates of synthesis of both protein and polysaccharide of the matrix, more severe in the steroid-treated group than in the starved controls. T h e changes were proportional to the duration of the study and the severity of the systemic catabolic state, as measured by weight loss. O n the basis of these observations, it is possible to speculate on the effect of systemically administered corticosteroids on rabbit articular cartilage and the relationship of this response to starvation and negative nutritional balance. MATERIALS AND METHODSAdult New Zealand white (NZW) rabbits were obtained Study 1Forty N Z W r...

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Effect of Cortisone upon Chondroitin Sulfate Synthesis by Animal Tissues.

Cited by 124 publications

References 0 publications

The Effect of Hydrocortisone and of Piromen in Vitro on Leukocytes of Patients Receiving Acth and Cortisone Therapy 1

The Effect of Hydrocortisone and of Piromen in Vitro on Leukocytes of Patients Receiving Acth and Cortisone Therapy 1

Labeling Index and Cellular Density in Palatine Shelves of Cleft-Palate Mice

The effect of systemic corticosteroids on rabbit articular cartilage

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