Effect of Cortisone on Reaction of Skin to Ultra-violet Light

Järvinen, Klaus A. J.

doi:10.1136/bmj.2.4744.1377

Cited by 35 publications

(6 citation statements)

References 4 publications

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“…The presumed in vivo activation of lysosomal enzymes by vitamin A acid in the larvae of Xenopus laevis is also inhibited by the simultaneous administration of hydrocortisone (34). Similarly, pretreatment with hydrocortisone protects skin in vivo (35) and in vitro (31) against the vesication and digestion of intercellular connective tissue which follow ultraviolet irradiation. Thus glucocorticoids, in a variety of experimental situations, appear to decrease the liberation of potentially harmful enzymes from lysosomes, and may in fact function physiologically to stabilize the boundaries of these subcellular particles.…”

Section: The Release Of Hydrolases From the Large Granule Fraction Ofmentioning

confidence: 99%

Studies on Lysosomes

Weissmann

Thomas

1962

The Journal of Experimental Medicine

286

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Granular fractions sedimenting between 800 g and 15,000 g have been prepared in 0.25 M sucrose from the livers of normal young rabbits and from the livers of rabbits injected with A. aerogenes endotoxin. As early as 5 minutes after endotoxin, there was an augmented release of two enzymes, beta glucuronidase and cathepsin, into the supernatant of fractions which had been incubated or incubated and irradiated with a mercury vapor lamp at 37°C. These effects were maximum at 30 minutes after endotoxin and were associated with a depletion of the total activities of the two enzymes within the granules. Concurrently there was a rise in the activity of the two enzymes in fractions of the homogenate which were unsedimentable at 15,000 g. Fractions prepared from animals made tolerant to endotoxin no longer responded to incubation and irradiation by an augmented release of these two hydrolases, nor did fractions prepared from animals pretreated with glucocorticoids. Pretreatment with DOCA did not prevent release of enzymes after endotoxin. The results have been interpreted to indicate that one consequence of the injection of endotoxin is an effect upon the stability of lysosomes, with subsequent release of acid hydrolases into the cell sap or surrounding tissue. Glucocorticoids may exert their pharmacologic effects through the protection of these subcellular particles against a variety of injurious agents.

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Section: The Release Of Hydrolases From the Large Granule Fraction Ofmentioning

confidence: 99%

Studies on Lysosomes

Weissmann

Thomas

1962

The Journal of Experimental Medicine

286

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“…It has been known for a long time that glucocorticoids can inhibit UVB‐induced erythema in vivo 16 –18 . Addition of hydrocortisone, prednisolone or dexamethasone at concentrations in the physiological (10 −9 –10 −7 mol/L) as well as pharmacological (10 −7 –10 −5 mol/L) range given immediately after UV exposure of epidermal cells blocked IL‐6 or IL‐1 release by UVB in vitro 5 .…”

Section: Discussionmentioning

confidence: 99%

“…[13][14][15] It has been known for a long time that glucocorticoids can inhibit UVB-induced erythema in vivo. [16][17][18] Addition of hydrocortisone, prednisolone or dexamethasone at concentrations in the physiological (10 ¹9 -10 ¹7 mol/ L) as well as pharmacological (10 ¹7 -10 ¹5 mol/L) range given immediately after UV exposure of epidermal cells blocked IL-6 or IL-1 release by UVB in vitro. 5 This could also be shown in our experiments, dexamethasone at an intermediate concentration of 10 ¹6 mol/L administered combined before and after UV irradiation having the greatest effect ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Ultraviolet B radiation-induced production of interleukin 1α and interleukin 6 in a human squamous carcinoma cell line is wavelength-dependent and can be inhibited by pharmacological agents

Eberlein-König

Jäger

Przybilla

1998

British Journal of Dermatology

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Ultraviolet (UV) B irradiation induces keratinocytes to produce among others the proinflammatory cytokines interleukin (IL) 1 and IL-6. The wavelength dependence of this UVB effect has not yet been assessed. We evaluated the potential of different UVB wavelength regions to release cytokines from the squamous carcinoma cell line SCL II and also assessed the effect of various putative inhibitors. Confluent monolayers of the cells were irradiated with 0.5-2.0 mJ/cm2 UVB at 280, 290, 300, 310 or 320 (each +/- 5) nm. In additional experiments dexamethasone (10-9-10-5 mol/L), ascorbic acid, d-alpha-tocopherol or indomethacin (each 10-7-10-4 mol/L) were added to the culture medium 24 h before, immediately after or combined before and after irradiation with 1 mJ/cm2 UVB at 280 +/- 5 nm. Supernatants of the cell cultures were recovered at 24 h after irradiation, and IL-1alpha or IL-6 were determined by an enzyme-linked immunosorbent assay (ELISA). IL-1alpha and IL-6 production were induced by UVB at 280, 290 and 300 nm, the production depended on the UV dose and decreased with increasing wavelengths. Irradiation at 310 or 320 nm did not induce cytokine production up to the maximum dose used. The production of IL-1 alpha/IL-6 was inhibited up to 80/89% (10-7-10-6 mol/L before and after irradiation) by dexamethasone in a concentration-dependent manner and with all conditions of incubation. Release of cytokines was also suppressed by indomethacin, d-alpha-tocopherol or ascorbic acid, but concentration dependence was not always evident. These results show that particularly shorter UVB radiation, which is expected to increase due to stratospheric ozone depletion, induces prominent production of proinflammatory cytokines, indicating major biological effects. Different pharmacological compounds can interfere with this effect and seem worth further evaluation with regard to their clinical effects.

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“…Thus, a coincidence between the in vitro and in vivo actions of vitamin A as a labilizer and cortisol as a stabilizer can conceivably be fortuitous. However, on morphologic grounds, too, protection of lysosomes by glucocorticoids has been shown against hypervitaminosis A in vivo (8,9) and in vitro (10), and against ultraviolet irradiation in vivo (34) and in vitro (35). Desoxycorticosterone acetate was not effective in such systems.…”

Section: Discussionmentioning

confidence: 99%