Intraperitoneal injection of 2.5% hydrocortisone suspension (0.5 ml) changes the content of biogenic amines in different lobule zones and thymocytes of high animals. The content of norepinephrine and serotonin increases in luminescent cells of the subcapsular and premedullary zones and decreases in mast cells.Key Words: hydrocortisone; thymus; subcapsular and premedullary zones; mast cells; serotonin; Hydrocortisone (cortisol, HC) is the most potent natural glucocorticoid in animals and men [ 1,4,9,11 ].The reticuloendothelial system (thymus, spleen, lymph nodes, etc.), muscular, bone, and connective tissue are the targets for glucocorticoids.Glucocorticoids (cortisol, cortisone, etc.) exhibit anti-inflammatory, antishock, antiallergic, and immunosuppressive activities, which are of importance in tissue and organ transplantation for prevention of their rejection. An important role in these processes is played by the thymus [5][6][7][8].Hydrocortisone exerts an antimitotic effect on cortical lymphocytes of the thymic lobules and induces a dose-dependent involution of the thymus [5]. This is accompanied by a 50% reduction of organ weight and its DNA content, inhibition of lymphocyte differentiation, and intensification of destructive processes.Numerous aspects of the molecular effect of steroid hormones on the cell and its nucleus have been extensively studied: transport into the cell, binding to receptor proteins, and formation of glucocorticoid-protein complexes followed by binding to specific DNA regions near the transcription initiation sites [1][2][3].However, little is known on the effect of HC on the content of biogenic amines in different thymic zones and cells [7,8].