Effect of corticosteroid treatment on interleukin‐1 and tumour necrosis factor secretion by monocytes from subjects with asthma

Vecchiarelli, Anna; Siracusa, Andrea; Cenci, Elio; Puliti, Manuela; Abbritti, Giuseppe

doi:10.1111/j.1365-2222.1992.tb03097.x

Cited by 36 publications

(18 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…First, the manifestations of asthma are, at least in part, channeled through the actions of IL-1␤ and TNF-␣ (11,12). Second, the efficacy of GCs in asthma is, at least in part, through its effect on the expression of genes that are modulated by proinflammatory cytokines (12,13). In this study, we show that GC responders can be separated from nonresponders with meaningful accuracy by using the expression levels of only a few genes.…”

mentioning

confidence: 86%

Profiling of genes expressed in peripheral blood mononuclear cells predicts glucocorticoid sensitivity in asthma patients

Hákonarson

Björnsdóttir

Halapi

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

150

105

View full text Add to dashboard Cite

Gene expression profiles were examined in freshly isolated peripheral blood mononuclear cells (PBMC) from two independent cohorts (training and test sets) of glucocorticoid (GC)-sensitive (n ‫؍‬ 64) and GC-resistant (n ‫؍‬ 42) asthma patients in search of genes that accurately predict responders and nonresponders to inhaled corticosteroids. A total of 11,812 genes were examined with high-density oligonucleotide microarrays in both resting PBMC (106 patients) and cells treated in vitro with IL-1␤ and TNF-␣ combined (88 patients), with or without GC. A total of 5,011 genes were expressed at significant levels in the PBMC, and 1,334 of those were notably up-regulated or down-regulated by IL-1␤͞TNF-␣ treatment. The expression changes of 923 genes were significantly reversed in GC responders in the presence of GC. The expression pattern of 15 of these 923 genes that most accurately separated GC responders (n ‫؍‬ 26) from the nonresponders (n ‫؍‬ 18) in the training set, based on the weighted voting algorithm, predicted the independent test set of equal size with 84% accuracy. The expression accuracy of these genes was confirmed by real-time-quantitative PCR, wherein 11 of the 15 genes predicted GC sensitivity at baseline with 84% accuracy, with one gene predicting at 81% in an independent cohort of 79 patients. We conclude that we have uncovered gene expression profiles in PBMC that predict clinical response to inhaled GC therapy with meaningful accuracy. Upon validation in an independent study, these results support the development of a diagnostic test to guide GC therapy in asthma patients.

show abstract

mentioning

confidence: 86%

Profiling of genes expressed in peripheral blood mononuclear cells predicts glucocorticoid sensitivity in asthma patients

Hákonarson

Björnsdóttir

Halapi

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

150

105

View full text Add to dashboard Cite

show abstract

“…TNF-u and IL-I~are key regulators of immune and inflammatory genes (24)(25). Conversely, IL-4 is a pivotal cytokine that is produced by Th2 cells and associated with allergic diseases that support Th2 development (26)(27).…”

Section: Discussionmentioning

confidence: 99%

A New Compound, 1H,8H-Pyrano[3,4-C]Pyran-1,8-Dione, Suppresses Airway Epithelial Cell Inflammatory Responses in a Murine Model of Asthma

Lee

Han

Kim

et al. 2009

Int J Immunopathol Pharmacol

View full text Add to dashboard Cite

Clinical and experimental studies have established eosinophilia as a sign of allergic disorders. Activation of eosinophils in the airways is believed to cause epithelial tissue injury, contraction of airway smooth muscle and increased bronchial responsiveness. As part of the search for new antiasthmatic agents produced by medicinal plants, the effects of 270 standardized medicinal plant extracts on cytokine-activated A549 human lung epithelial cells were evaluated. After several rounds of activityguided screening, the new natural compound, IH,8H-Pyrano[3,4-c]pyran-l,8-dione (PPY), was isolated from Vitex rotundifolia L. To elucidate the mechanism by which the anti-asthmatic responses of PPY occurred in vitro, lung epithelial cells (A549 cell) were stimulated with TNF-a, IL-4 and IL-lP to induce the expression of chemokines and adhesion molecules involved in eosinophil chemotaxis. PPY treatments reduced the expression of eotaxin, IL-8, IL-16 and VCAM-l mRNA significantly. Additionally, PPY reduced eotaxin secretion in a dose-dependent manner and significantly inhibited eosinophil migration toward A549 medium. In addition, PPY treatment suppressed the phosphorylation of p65 and ERKl/2, suggesting that it can inhibit the MAPKlNF-KB pathway. To clarify the anti-inflammatory and antiasthmatic effects of PPY in vivo, we examined the influence of PPY on the development of pulmonary eosinophilic inflammation in a murine model of asthma. To accomplish this, mice were sensitized and challenged with ovalbumin (OVA) and then examined for the following typical asthmatic reactions: an increase in the number of eosinophils in BALF; the presence of Th2 cytokines such as IL-4 and IL-5 in the BALF; the presence of allergen-specific IgE in the serum; and a marked influx of inflammatory cells into the lung. Taken together, our results revealed that PPY exerts profound inhibitory effects on the accumulation of eosinophils into the airways while reducing the levels of IL-4, IL-5, and IL-13 in the BALF. Therefore, these results suggest that PPY may be useful as a new therapeutic drug for the treatment of allergic asthma.Exposure to environmental hazards causes outbreaks ofallergic diseases, such as allergic asthma,

show abstract

“…Supernatants from PBM were obtained according to a previously described method [22]. Briefiy, PBM were seeded at a concentration of 2 x 10^/ml of cRPMI for 48 h without or with LPS (10/ig/mi) or Timothy grass pollen.…”

Section: Production Of Pbm Cutture Supernatants and Assaying For Tnf mentioning

confidence: 99%

Cytokine regulation of low-affinity IgE receptor (CD23) on monocytes from asthmatic subjects

Vecchiarelli

Siracusa

Monari

et al. 1994

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SUMMARYThe regulation of CD23 expression (FcfRII) by cytokines on monocytes from normal subjects, asymptomatic and acute asthmatics was investigated. CD23 was weakly expressed on cells from controls, but was significantly enhanced in the two groups of asthmatics. The addition of IL-4 on monocytes induced an increase of CD23 expression in cells from controls and asthmatics. Interferon-gamma (IFN-7) did not modulate CD23 expression in asthmatics or control subjects, while high doses of IL-6 (2000 U/ml) enhanced CD23 expression on cells from asthmatics or controls. In vitro stimulation of monocytes with Timothy grass pollen allergen did not enhance CD23 receptor in asthmatics with a positive skin test to this pollen. We speculate that CD23 expression in asthmatics is markedly enhanced by Th2-dependent cytokines, such as IL-4 and IL-6. Thus, the regulation of Th2 cell activation by anti-cytokine therapy could have an important effect on the down-regulation of CD23 on monocytes. and in shifting a Th2 subpopulation into a Thl subpopulation by blocking Th2-dependent cytokines.

show abstract

Effect of corticosteroid treatment on interleukin‐1 and tumour necrosis factor secretion by monocytes from subjects with asthma

Cited by 36 publications

References 27 publications

Profiling of genes expressed in peripheral blood mononuclear cells predicts glucocorticoid sensitivity in asthma patients

Profiling of genes expressed in peripheral blood mononuclear cells predicts glucocorticoid sensitivity in asthma patients

A New Compound, 1H,8H-Pyrano[3,4-C]Pyran-1,8-Dione, Suppresses Airway Epithelial Cell Inflammatory Responses in a Murine Model of Asthma

Cytokine regulation of low-affinity IgE receptor (CD23) on monocytes from asthmatic subjects

Contact Info

Product

Resources

About