EFFECT OF COPPER SULPHATE AND COBALT CHLORIDE ON GROWTH OF THE <i>IN VITRO</i> CULTURE TISSUES FOR DATE PALM (<i>PHOENIX DACTYLIFERA</i> L.) CV. ASHGAR

Abstract

This study was carried out to investigate the effects of copper sulphate and cobalt chloride on propagation of date palm cv. Ashgar in vitro. The rate of callus proliferation was significantly higher in the medium supplemented with 2 µM copper sulphate and 2 µM cobalt chloride together (p<0.05). Addition of copper sulphate and Cobalt chloride to the medium was most effective for shoots regeneration from callus and enhanced regeneration frequency as well as number of shoots obtained per explant, the best result (7.12 shoot/explant) was obtained by using copper sulphate and Cobalt chloride at 2.0 µM. Also the results of this experiment indicates that the maximum induction of roots can be achieved in the media containing both additives at 0.5 µM. The callus exhibited a decline in carbohydrate contents, proteins, whereas total phenol content increased at high concentration of Cu and Co. The greatest formation of chlorophylls a, b and total chlorophyll was obtained in plantlets grown in the media containing both additives at 0.5 µM. As well as Maximum survivability was noticed for plants cultured in media that containing both copper sulphate and Cobalt chloride at 0.5 µM.

IMPROVEMENT OF SILYMARIN CONTENT IN CELL CULTURES OF Silybum marianum BY COPPER SULPHATE ELICITOR

“…Callus proliferation of date palm was significantly enhanced due to copper sulphate elicitor in the medium and shoot regeneration was also improved. In addition, shoot number was increased per explant and the total phenol content was higher compared with the control [AL-Mayahi 2014].…”

Effect of aluminum on the growth of the in vitro culture tissues of the date palm (Phoenix dactylifera L.) cv. Um-Aldehin

“…The increase in APX activity can be due to stress caused by changes in the media composition, where Al induces a stress response (Siripornadulsil et al 2002, Vardar et al, 2006. The accumulation of excessive quantities of heavy metals in plant tissues of causes changes in physiological processes, including the biosynthesis of chlorophyll (Hussian et al, 2010;Al-Mayahi, 2014). Chlorophyll a fluorescence has been described by Kalaji et al (2016) as an important tool for monitoring the physiological state of plants growing under different environmental stress conditions.…”

“…Tissue cultures were used in many studies on plant response to metal ions, with conclusions that the success of plant propagation by tissue cultures was highly influenced by the composition of the culture medium used (Al-Mayahi, 2012 and2014). The data showed that AlCl 3 treatments induced and propelled differentiation of friable callus explants of the date palm cv.…”

Micronutrient Delivery System for Induction of Organogenesis in Banana in vitro Cultures

“…There are several factors that influence in vitro growth of tissues and plants, including plant growth regulators, temperature, and light quality [4,5,6]. The culture medium greatly influences the success of in vitro techniques as a means of micropropagation and the optimization of the medium is genotype dependent [7,8,9] Healthy and vigorous growth of plants requires efficient nutrient uptake by in vitro cultures and micronutrients have been shown to play important roles in plant regeneration [10]. Metals, such as cobalt, iron, manganese, copper and zinc are essential for plants and required in very small or trace amounts [11].…”

“…Improved Zn uptake can result in increased crop yields [17,18]. Under in vitro conditions, optimum Cu and Zn concentrations in the culture medium positively affect plant development [19,10]. However, studies on in vitro mineral nutrition, specifically with micronutrients, are still limited [4,20,21].…”