Effect of Copper Oxide and Silver Nanoparticles on the Development of Tolerance to Alternaria alternata in Poplar in Vitro Clones

Гродецкая, Т. А.; Федорова, О. А.; Евлаков, Петр; Баранов, О. Ю.; Zakharova, Olga V.; Gusev, Alexander

doi:10.1134/s2635167621020063

Cited by 3 publications

(5 citation statements)

References 44 publications

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“…Exposure to CuO NPs caused a decrease in the level of PAL gene transcripts, while a positive effect of CuO NPs on the expression of this gene in cabbage roots and seedlings [ 67 ], soybean roots [ 40 ], and cucumber shoots [ 68 ] was shown. Previous research revealed the activation of DREB2 under the influence of A. alternata exposed to CuO and Ag NPs, as well as under the separate action of NPs on poplar microclones [ 43 ]; however, our results indicate a decrease in the expression of DREB2 gene in birch microclones in all the experimental variants. The observed effects can be explained by the genotypic features of downy birch and the selected concentration of NPs, although a more detailed study of the NPs effect on the genes expression of woody plants is needed.…”

Section: Discussioncontrasting

confidence: 80%

“…The plants were maintained in a Binder KBW 400 (E5.1) climatic chamber (Binder, Tuttlingen, Germany) with the following climatic conditions: a 16-h photoperiod at an illumination of 30 μm/m −2 ·s −1 and a temperature of 24 ± 2 °C. Artificial infection of the microclones was carried out according to the method presented earlier [ 43 ]. The fungus culture at a concentration of 2 × 10 6 spores/mL was sprayed onto the birch clones and simultaneously introduced into the cultivation media with and without NPs.…”

Section: Methodsmentioning

confidence: 99%

“…The primers for resistance genes [ 43 , 47 , 48 , 49 , 50 ] were selected based on sequences from the NCBI database using the Primer3 program ( Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

“…Previously, we revealed the positive effects of 50 nm thick copper oxide nanoplates on poplar microshoots during in vitro cultivation. However, the mechanisms of the plant responses formation at the genes expression level in woody plants remained unclear [ 43 ].…”

Section: Introductionmentioning

confidence: 99%

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Influence of Copper Oxide Nanoparticles on Gene Expression of Birch Clones In Vitro under Stress Caused by Phytopathogens

et al. 2022

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Recently, metal oxide nanoparticles (NPs) have attracted attention as promising components for the protection and stimulation of plant microclones in tissue culture in vitro. However, the effect of NPs on the genetic mechanisms underlying plant adaptive responses remains poorly understood. We studied the effect of column-shaped CuO NPs 50 nm in diameter and 70–100 nm in length at a concentration of 0.1–10 mg/L on the development of phytopathogenic fungi Alternaria alternata, Fusarium oxysporum, and Fusarium avenaceum in culture, as well as on the infection of downy birch micro-clones with phytopathogens and the level of genes expression associated with the formation of plant responses to stress induced by microorganisms. CuO NPs effectively suppressed the development of colonies of phytopathogenic fungi A. alternata and F. avenaceum (up to 68.42% inhibition at 10 mg/L CuO NPs) but not the development of a colony of F. oxysporum. Exposure to the NPs caused multidirectional responses at the level of plant genes transcription: 5 mg/L CuO NPs significantly increased the expression level of the LEA8 and MYB46 genes and decreased the expression of DREB2 and PAL. Infection with A. alternata significantly increased the level of MYB46, LEA8, PAL, PR-1, and PR-10 transcripts in birch micro-clones; however, upon exposure to a medium with NPs and simultaneous exposure to a phytopathogen, the expression of the MYB46, PR-1, and PR-10 genes decreased by 5.4 times, which is associated with a decrease in the pathogenic load caused by the effect of NPs and the simultaneous stimulation of clones in vitro. The results obtained can be used in the development of preparations based on copper oxide NPs for disinfection and stimulation of plant phytoimmunity during clonal micropropagation of tree crops.

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Section: Discussioncontrasting

confidence: 80%

Section: Methodsmentioning

confidence: 99%

“…The primers for resistance genes [ 43 , 47 , 48 , 49 , 50 ] were selected based on sequences from the NCBI database using the Primer3 program ( Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Influence of Copper Oxide Nanoparticles on Gene Expression of Birch Clones In Vitro under Stress Caused by Phytopathogens

et al. 2022

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“…The hazards of Alternaria to plants are sufficient to explain and it is one of the main groups causing plant pathogen diseases [ 9 ]. Many researchers [ 10 ] have also proved that poplar can respond to the infection of Alternaria . Therefore, the study of the impact of Alternaria on poplar is of great significance for the study of poplar disease resistance.…”

Section: Introductionmentioning

confidence: 99%

Sorbitol Reduces Sensitivity to Alternaria by Promoting Ceramide Kinases (CERK) Expression through Transcription Factor Pswrky25 in Populus (Populus simonii Carr.)

Song

et al. 2022

Genes

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Sugar, acting as a signal, can regulate the production of some chemical substance during plant defense responses. However, the molecular basis and regulatory mechanisms of sugar in poplar and other forest trees are still unclear. Sorbitol is a sugar-signaling molecule associated with plant defense. In this study, the pathogen-infested status of poplar was alleviated after exogenous feeding of 50 mM sorbitol. We sequenced and analyzed the transcriptome of poplar leaves before and after inoculation. The results showed that the genes PR1, WRKY, ceramide kinases (CERK) and so on responded to sorbitol feeding and pathogen infestation. We screened for genes related to disease resistance such as PsWRKY25 and PsCERK1 and found that significant disease spots occurred on day six of strep throat infestation. Under sorbitol feeding conditions, the appearance of spots was delayed after the pathogen inoculation. Due to the overexpression of PsWRKY25, the overexpression of PsCERK1 triggered the defense response in poplar. This was also confirmed by PsWRKY25 overexpression experiments. These findings present new insights into the influence of sorbitol on Populus simonii Carr. disease resistance. These results emphasize the value of molecular phenotypes in predicting physiological changes.

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Effect of Multi-Walled Carbon Nanotubes on the Growth and Expression of Stress Resistance Genes in Birch

Жужукин

Евлаков

Grodetskaya

et al. 2023

Forests

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Recent studies have shown that nanomaterials, including carbon nanotubes, are associated with a wide range of effects on living organisms, from stimulation to toxic effects. Plants are an important object of such research, which is associated with the potential use of carbon nanomaterials in agriculture and environmental protection. At the same time, the specific mechanisms of formation of plant resistance to the effects of carbon nanotubes remain not fully understood, especially in woody plants. Therefore, we studied the effect of aqueous colloids of multi-walled carbon nanotubes (MWCNTs) with an outer diameter of 10–30 nm and a length of about 2 μm at a concentration of 1, 10, 50, and 100 mg/L on morphometric parameters and the level of expression of stress resistance genes in Betula pubescens Ehrh. and B. pendula Roth. plants in greenhouse conditions. The results showed an increase in the length and diameter of the shoot in the studied plants. The dry biomass of the leaf increased by 30%, the stem by 42%, and the root by 49% when using MWCNTs at a concentration of 10 mg/L. The expression of the stress resistance genes DREB2 and PR-10 significantly increased under the influence of 1 mg/L MWCNTs on plants of both species. At the same time, the use of 100 mg/L nanoparticles led to a decrease in the studied parameters in Betula pendula, which may be associated with the negative effect of MWCNTs in high concentrations. The revealed positive effects of low concentrations of MWCNTs on morphometric parameters and stimulation of stress resistance genes by nanotubes open up prospects for their use in woody plant biotechnology.

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Effect of Copper Oxide and Silver Nanoparticles on the Development of Tolerance to Alternaria alternata in Poplar in Vitro Clones

Cited by 3 publications

References 44 publications

Influence of Copper Oxide Nanoparticles on Gene Expression of Birch Clones In Vitro under Stress Caused by Phytopathogens

Influence of Copper Oxide Nanoparticles on Gene Expression of Birch Clones In Vitro under Stress Caused by Phytopathogens

Sorbitol Reduces Sensitivity to Alternaria by Promoting Ceramide Kinases (CERK) Expression through Transcription Factor Pswrky25 in Populus (Populus simonii Carr.)

Effect of Multi-Walled Carbon Nanotubes on the Growth and Expression of Stress Resistance Genes in Birch

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