Effect of copper ions concentration on the particle size of alginate-stabilized Cu₂O-Cu nanocolloids and its antibacterial activity against rice bacterial leaf blight (Xanthomonas oryzae pv. oryzae)

Abstract

In this study, nano Cu2O-Cu/alginate was synthesised by the chemical reduction of tetra-amminecopper (II) ion complexes soluble using hydrazine as a reducing agent and alginate as a stabiliser. The influence of various copper ion concentrations on particle size and morphology of produced nano Cu2O-Cu/alginate was investigated. The transmission electron microscopy (TEM) analysis and X-ray diffraction (XRD) measurement revealed that the synthesised nano Cu2O-Cu/alginate was mostly composed of Cu2O-Cu in the form of a Cu2O@Cu core–shell structure with particle sizes of 4.2, 5.3, and 8.4 nm for 60, 80, and 100 mM of copper ion initial concentrations, respectively. The obtained nano Cu2O-Cu/alginate exhibited high antibacterial activity against Xanthomonas oryzae pv. Oryzae (Xanthomonas OO). The results showed that the nano Cu2O-Cu/alginate containing 30 mg.l−1 of copper ions completely inhibited the growth of Xanthomonas OO bacteria, which are responsible for the rice leaf blight disease. Nano Cu2O-Cu/alginate materials can be used as a fungicide to replace toxic agro-chemicals and potentially applied to the development of sustainable agricultural production.

Investigation of the antibacterial mechanism of the novel bactericide dioctyldiethylenetriamine (Xinjunan)

“…oryzae (Xoo) is one of the most devastating rice diseases in major ricegrowing regions worldwide. BLB occurs in many countries, [7][8][9] including China, 10 Indonesia, 11 Vietnam 12,13 and Japan. 14 BLB has seriously affected rice production and caused economic losses in these countries.…”

Synthesis of Nano Sulfur/Chitosan-Copper Complex and Its Nematicidal Effect against Meloidogyne incognita In Vitro and on Coffee Pots

“…sarium oxysporum (Kaman and Dutta, 2019), nano MgO, nano Cu 2 O-Cu, nano ZnO, MnO 2 resistant to Xanthomonas oryzae pv. oryzae (Abdallah et al, 2019;Ngoc et al, 2021;Ogunyemi et al, 2020), and nano Ag resistant to yellow bean mosaic virus (Elbeshehy et al, 2015).…”

“…Nanomaterials have the ability to resist a variety of plant pathogens such as nano SiO 2 /oligochitosan, nano Cu 2 O-Cu/alginate resistant to Neoscytalidium dimidiatum ( Du et al, 2019 ; Tuan et al, 2019 ), nano SiO 2 resistant to Rhizoctonia solani ( Abdelrhim et al, 2021 ), nano Ag resistant to Fusarium oxysporum ( Kaman and Dutta, 2019 ), nano MgO, nano Cu 2 O-Cu, nano ZnO, MnO 2 resistant to Xanthomonas oryzae pv. oryzae ( Abdallah et al, 2019 ; Ngoc et al, 2021 ; Ogunyemi et al, 2020 ), and nano Ag resistant to yellow bean mosaic virus ( Elbeshehy et al, 2015 ).…”