Effect of Copper and Lindane on Some Biomarkers Measured in the Clam Ruditapes decussatus

Hamza-Chaffai, Amel; Roméo, Michèle; Gnassia‐Barelli, M.; Abed, Amor El

doi:10.1007/s001289900776

Cited by 64 publications

(31 citation statements)

References 11 publications

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“…These results are consistent with previous studies conducted in this region. In fact, the pollution in this coast is due essentially to the presence of continuous discharge of heavy metals from local industrial activities (Hamza-Chaffai et al 1997, 1998 and from the phosphogypsum stock (Illou 1999;Serbaji 2000). Several reports have also indicated high metal concentrations in soft tissues of Reditapes decussatus (Smaoui-Damak et al 2003;Hamza-Chaffai and Pellerin 2003) and in the fish Scorpaena porcus (Hamza-Chaffai et al 1995) from this coast.…”

Section: Discussionmentioning

confidence: 99%

Association of spinal deformities with heavy metal bioaccumulation in natural populations of grass goby, Zosterisessor ophiocephalus Pallas, 1811 from the Gulf of Gabès (Tunisia)

Messaoudi

Deli

Kessabi

et al. 2008

Environ Monit Assess

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The present study illustrates an analysis of spinal deformities associated with metal accumulation in natural populations of Zosterisessor ophiocephalus derived from polluted (S1) and unpolluted (S2) areas in the Gulf of Gabès in Tunisia. Three basic types of spinal deformities were detected: kyphosis, scoliosis and lordosis. These basic deformities frequently co-occur. Spinal deformities were observed in 10.72% of the total examined fish (n = 494). Deformed fish were 3.85 times more frequent in S1 than in S2. In both sexes, the highest occurrence of deformities was observed in the 111-120 mm class decreasing thereafter with fish length. Hepatic concentrations of cadmium (Cd), copper (Cu) and zinc (Zn) were significantly higher in Z. ophiocephalus from S1 than those from S2. Comparisons between fish in each site showed that liver concentrations of Cd and Zn were significantly higher in deformed fish than in normal fish. The relationship between metals accumulation and observed spinal deformities as well as the suitability of this kind of studies for environmental monitoring are discussed.

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Section: Discussionmentioning

confidence: 99%

Association of spinal deformities with heavy metal bioaccumulation in natural populations of grass goby, Zosterisessor ophiocephalus Pallas, 1811 from the Gulf of Gabès (Tunisia)

Messaoudi

Deli

Kessabi

et al. 2008

Environ Monit Assess

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“…Therefore it was observed that the increase in Cu in the soluble fraction was not correlated with MT concentrations [8]. Cu was also shown to increase the lipid peroxidation of membranes in the digestive gland of R. decussatus.…”

Section: Mt Induction In Laboratory Experimentsmentioning

confidence: 95%

“…Several laboratory experiments have been carried out to assess MT induction in R. decussatus exposed to sublethal concentrations of cadmium (100 and 400 µg/l), copper (75 µg/l) and lindane (34.5 µg/l) [4,7,6,8].…”

Section: Mt Induction In Laboratory Experimentsmentioning

confidence: 99%

Metallothioneins in the clam Ruditapes decussatus: an overview

Bebianno¹,

Serafim²,

Simes³

2000

Analusis

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The clam R. decussatus is a suspension-feeding bivalve mollusc widely distributed in European and Mediterranean coastal waters. Due to its economic importance it is extensively cultured in many countries, and particularly in Portugal where its production represents > 80 % of total shellfish production. The selection of the clam R. decussatus as a bioindicator was a consequence of its economic importance and the need to use a filter-feeding bivalve to evaluate environmental changes of metal concentrations [1].Like many other bivalves, this species resides in sediments and accumulates metal concentrations reflecting gradients of metal contamination in the surrounding environment [2,3,4]. This suggests that its tissues have mechanisms, related to its filter-feeding habit, that inhibit the toxic effects of these contaminants.Like many other molluscs R. decussatus has evolved a number of subcellular systems for accumulation, regulation and immobilization of metals. These include, among others, the binding of essential and pollutant metals to soluble ligands such as MT. These two-domain molecules are low molecular, heat-stable proteins of non-enzymatic nature that occur mainly in the cytoplasm. They have strong affinity to class B metal cations which enable them to be differentiated from most of the other proteins [5]. The induction of these proteins has, therefore, been proposed as a specific indicator and possible "early warning marker" for the detection of detrimental effects caused by exposure to excess of essential and pollutant metals.MT induction has been detected in the whole soft tissues, gills, digestive gland and remaining tissues of the clam R. decussatus after exposure to essential and toxic metals [4,6] and this paper represents an overview of MT function in this bivalve species. MT induction in laboratory experimentsSeveral laboratory experiments have been carried out to assess MT induction in R. decussatus exposed to sublethal concentrations of cadmium (100 and 400 µg/l), copper (75 µg/l) and lindane (34.5 µg/l) [4,7,6,8].Chromatographic elution profiles of the heat-treated cytosol of the whole soft tissues of unexposed clams revealed that cadmium in this pool (> 80 % of total Cd) was distributed between two cadmium-binding proteins of a molecular weight of around 10,000 and 20,000 Da, respectively, that increased with Cd accumulation. Similarly an increase in the -SH containing proteins (by 70 % compared with unexposed clams), based on differential pulse polarography Similarly the amino acid sequence of the β domain of the MT of the digestive gland of the clam also shows some degree of similarity with the similar MT sequence from mussels and oysters. It is, therefore suggested that there is some degree of similarity in the MT structure among these species.

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“…Acetylcholinesterase activity which is known to be inhibited by the presence of organophosphorus compounds and carbamates is considered an exposure biomarker for these substances (Galgani and Bocquené 1989). Some others studies demonstrated that acetylcholinesterase is also inhibited by heavy metals in various organisms (Bocquené et al 1997), in mussels (Najimi et al 1997) and in clams (Hamza-Chaffai et al 1998). In addition to these data on chemical bioaccumulation and biochemical effect, the impact on organisms was also assessed using a general index, the condition index, which reflects the physiological status of the studied animals.…”

Section: Introductionmentioning

confidence: 94%

The effect of in situ cadmium contamination on some biomarkers in Cerastoderma glaucum

et al. 2007

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The present study was designed to detect in situ effects of cadmium on marine organisms Cerastoderma glaucum from the gulf of Gabès (Tunisia). Six sampling stations were chosen: one site, relatively far from known local source of pollution, was considered as uncontaminated site and five sites clearly exposed to anthropogenic impact. Metallothionein like protein (MTLP) and sub-cellular metal partitioning were measured in cockles (C. glaucum) gills and digestive gland. Various biomarkers were also measured, including malondialdehyde (MDA) in the digestive gland and acetylcholinesterase activity (AChE) in the remainder. The health status of the cockles was assessed by using the condition index (CI). Significant differences between sites were noted for Cd concentrations, (CI) and also for the three studied biomarkers. Significant higher biomarkers response was measured in cockles from stations located in the northern part of the gulf, which are exposed directly to industrial and urban effluents, whereas the response of most biomarkers was minimal at the reference station. Positive and significant correlations were observed between MTLP and Cd concentrations in the digestive gland and in the gills. However, it must be noted that these correlations were more significant in the digestive gland, suggesting that compared to the gills, the digestive gland of C. glaucum is more suitable for monitoring metal pollution. The subcellular distribution of Cd showed that the soluble fraction was the major compartment for Cd storage, a pattern which is due to the role of MTLP in Cd detoxication. But at the most contaminated site (EH), cadmium in the digestive gland was preferentially accumulated in the insoluble fraction (P1) suggesting that the MTLP capacity in binding metals was not sufficient to avoid the binding of Cd to the insoluble fraction. Furthermore, the MTLP concentrations in the cockles from this site are lower than expected. So in highly polluted sites, MTLP in C. glaucum should not be used as a useful biomarker for metal pollution. The lipid peroxidation as presented by malondialdehyde levels, and MT-like protein concentrations increased in cockles exposed to cadmium contamination. We can, therefore, hypothesize that Cd could induce MTLP synthesis and MDA increase. While AChE had distinct and specific pattern showing that cadmium is not the only factor of the inhibition of cholinesterase activity. There are other polluting inputs engendering this inhibition.

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Effect of Copper and Lindane on Some Biomarkers Measured in the Clam Ruditapes decussatus

Cited by 64 publications

References 11 publications

Association of spinal deformities with heavy metal bioaccumulation in natural populations of grass goby, Zosterisessor ophiocephalus Pallas, 1811 from the Gulf of Gabès (Tunisia)

Association of spinal deformities with heavy metal bioaccumulation in natural populations of grass goby, Zosterisessor ophiocephalus Pallas, 1811 from the Gulf of Gabès (Tunisia)

Metallothioneins in the clam Ruditapes decussatus: an overview

The effect of in situ cadmium contamination on some biomarkers in Cerastoderma glaucum

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