EFFECT OF COOLED and FROZEN BOAR SEMEN ON EMBRYO DEVELOPMENT

Rillo, Santiago Martín; Pintado, B.; Alba, C. de; Sánchez-Sánchez, Raúl; García, P.; Corcuera, D.; Artiga, Carlos García; Sagüés, A.; Díaz, Carmen Pérez; Sáiz, F.; Pérez, Méndez

doi:10.1111/j.1439-0531.1996.tb00072.x

Cited by 6 publications

(1 citation statement)

References 2 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Es importante destacar que la técnica básica de evaluación espermática solo proporciona la información esencial para tomar decisiones sobre el uso de los eyaculados y/o dosis seminales conservadas. Esto es evidente en este trabajo, ya que las dosis seminales de verracos SPR y aún de verracos CPR estuvieron dentro de los parámetros de uso que Rillo et al (1996)…”

Section: Efecto Del Tipo De Alojamiento En La Reacción Acrosomalunclassified

Reacción acrosomal en espermatozoides de verracos con problemas reproductivos

Barragán

View full text Add to dashboard Cite

A la M. en C. Adelfa Del Carmen, por sus consejos y apoyo AI M. en C. Héctor Zayas, por su amistad incondicional al compartir sus conocimientos. AI M. en C. Jesús Conejo, por su amistad y ayuda desinteresada en la revisión del trabajo. AI M. en C. Ricardo Ruiz, por su gran apoyo y ayuda en la parte del análisis estadístico de lo datos. AI Dr. Heberto Esparza, por la revisión del trabajo.A todos los compañeros del laboratorio de Biología Celular, por el respeto y disposición de ayuda.

show abstract

Section: Efecto Del Tipo De Alojamiento En La Reacción Acrosomalunclassified

Reacción acrosomal en espermatozoides de verracos con problemas reproductivos

Barragán

View full text Add to dashboard Cite

show abstract

Acrosome Reaction in Fertile and Subfertile Boar Sperm

Herrera

Fierro

Zayas

et al. 2002

Archives of Andrology

View full text Add to dashboard Cite

The main purpose of sperm evaluation is to predict its fertilizing ability. However, basic sperm test results show a low correlation with fertilizing ability. The purpose of this study was to determine whether there is an association between acrosome reaction (AR) and the incidence of subfertility of normal sperm boar. The production records of 22 farms were analyzed to identify boars with low fertility and/or prolificity, classified as subfertile. Twenty-two subfertile boar semen samples were analyzed and compared with 51 samples of fertile boars. Sperm were capacitated during 4 h at 39 degrees C. viability was determined by bisbenzimide (Hoechst-33258) staining. Acrosome reaction was assessed with fluorescein isothiocyanate conjugated Pisum sativum agglutinin. The percentage of spontaneous acrosome reaction (SAR) was not significantly different in fertile (4.5%) and subfertile boars (4.75%) (p > .05). Nevertheless, the percentage of progesterone-induced acrosome reaction (IAR) was significantly lower in subfertile boars (5.75%) as compared with fertile boars (10%) (p < .01). These results suggest that assessment of IAR in vitro may be a useful parameter to identify subfertility in boars.

show abstract

Expression of Lectin Receptors on the Membrane Surface of Sperm of Fertile and Subfertile Boars by Flow Cytometry

Jiménez

González‐Márquez

Ortiz

et al. 2002

Archives of Andrology

View full text Add to dashboard Cite

Studies suggest that carbohydrates are important in different stages of fertilization. Plasma membrane changes accompanying in vitro capacitation and acrosome reaction (AR), such as removal or appearance of specific glycoproteins, have been studied using lectins that bind specifically to carbohydrate residues. In specialized artificial insemination farms and semen production centers, identification of boars with decreased fertilization ability (subfertility) is a newborn necessity. This investigation is a sequential study to determine the kinetics of surface carbohydrates turnover during in vitro capacitation and AR in fertile and subfertile boar sperm. Flow cytometry determinations of the binding of three FITC-labeled lectins were assessed. WGA binding was significantly lower in fresh, capacitated, and acrosome-reacted sperm of subfertile boars than in fertile boars. Con-A binding was not significantly different in fresh sperm of fertile and subfertile boars. However. Con-A labeling in capacitated, and acrosome-reacted sperm differed significantly in both groups. UEA binding increased only in capacitated sperm of subfertile boars. These findings could be used as indicators of capacitation and AR and may also be a good indicator of sperm fertilizing ability in boars.

show abstract

EFFECT OF COOLED and FROZEN BOAR SEMEN ON EMBRYO DEVELOPMENT

Cited by 6 publications

References 2 publications

Reacción acrosomal en espermatozoides de verracos con problemas reproductivos

Reacción acrosomal en espermatozoides de verracos con problemas reproductivos

Acrosome Reaction in Fertile and Subfertile Boar Sperm

Expression of Lectin Receptors on the Membrane Surface of Sperm of Fertile and Subfertile Boars by Flow Cytometry

Contact Info

Product

Resources

About