Effect of Conway Medium and f/2 Medium on the growth of six genera of South China Sea marine microalgae

Lananan, Fathurrahman; Jusoh, Ahmad; Ali, Nora’aini; Lam, Su Shiung; Endut, Azizah

doi:10.1016/j.biortech.2013.03.006

Cited by 79 publications

(43 citation statements)

References 30 publications

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“…The said concentration was 32.41 % higher than the cell density obtained by F/2 medium under the same conditions, surpassing the difference reported for Isochrysis sp. by Lananan et al (2013). Moreover, fucoxanthin concentration and total production was higher in Conway medium aerated cultures (~42.46 and~50.68 %, respectively), which proves the importance of medium composition and nutrient concentration.…”

Section: Agitationmentioning

confidence: 75%

“…A previous comparison of the growth of an Isochrysis species isolated from the South China Sea grown in Conway medium demonstrated a slightly higher maximum cell density (1.45 %) than cultures grown in F/2 medium, and in both media conditions, the maximum cell density was reached at day 17 of growth (Lananan et al 2013). However, the study does not report culture conditions, such as aeration/agitation, temperature or light; the study was solely focused on biomass production.…”

Section: Agitationmentioning

confidence: 82%

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Growth kinetics and fucoxanthin production of Phaeodactylum tricornutum and Isochrysis galbana cultures at different light and agitation conditions

2015

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Fucoxanthin is a carotenoid that exerts multiple beneficial effects on human health. However, reports comparing microalgae culture conditions and their effect on growth and fucoxanthin production are still limited. Isochrysis galbana and Phaeodactylum tricornutum cultures in different light (62.0, 25.9, 13.5, or 9.1 μmol photons m -2 s -1 ), mixing conditions (1 vvm aeration or 130 rpm agitation), and media compositions (F/2 and Conway medium) were studied for comparison of cellular growth and fucoxanthin production on F/2 medium. I. galbana showed a better adaptation to tested culture conditions in comparison with P. tricornutum, reaching 2.15 × 10 7 ±4.07×10 6 cells mL -1 and a specific growth rate (μ) of 1.12±0.05 day -1 under aerated conditions and 62.0 μmol photons m -2 s -1 light intensity. Fucoxanthin concentration was about 25 % higher in P. tricornutum cultures under 13.5 μmol photons m -2 s -1 light intensity and aerated conditions, but the highest fucoxanthin total production was higher in I. galbana, where 3.32 mg can be obtained from 1 L batch cultures at the 16th day under these conditions. Moreover, higher cell densities (~32.41 %), fucoxanthin concentration (~42.46 %), and total production (~50.68 %) were observed in I. galbana cultures grown in Conway medium, if compared with cultures grown in F/2 medium. The results show that the best growth conditions did not result in the best fucoxanthin production for either microalgae, implying that there is not a direct relationship between cellular growth and fucoxanthin production. Moreover, the results suggest that I. galbana cultures on Conway medium are strong candidates for fucoxanthin production, where 1.2 to 15 times higher fucoxanthin concentration are observed in comparison to macroalgal sources.

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Section: Agitationmentioning

confidence: 75%

Section: Agitationmentioning

confidence: 82%

Growth kinetics and fucoxanthin production of Phaeodactylum tricornutum and Isochrysis galbana cultures at different light and agitation conditions

2015

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“…Regarding culture media, C. reinhardtii and C. variabilis were cultivated in TAP medium and minimal medium (Harris, 1989) containing 20 mM MOPS. Other microalgae were grown in artificial sea water added with various nutrients: Conway's, Guillard's F/2 (Lananan et al, 2013) and Keller's (Keller et al, 1987) media were used for Nannochloropsis, P. tricornutum, and O. tauri, respectively. Cells were routinely counted using a Multisizer 3 (Coulter).…”

Section: Strains and Culture Conditionsmentioning

confidence: 99%

Microalgae Synthesize Hydrocarbons from Long-Chain Fatty Acids via a Light-Dependent Pathway

Sorigué

Légeret²,

Cuiné³

et al. 2016

Plant Physiol.

115

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Microalgae are considered a promising platform for the production of lipid-based biofuels. While oil accumulation pathways are intensively researched, the possible existence of a microalgal pathways converting fatty acids into alka(e)nes has received little attention. Here, we provide evidence that such a pathway occurs in several microalgal species from the green and the red lineages. In Chlamydomonas reinhardtii (Chlorophyceae), a C17 alkene, n-heptadecene, was detected in the cell pellet and the headspace of liquid cultures. The Chlamydomonas alkene was identified as 7-heptadecene, an isomer likely formed by decarboxylation of cis-vaccenic acid. Accordingly, incubation of intact Chlamydomonas cells with per-deuterated D 31 -16:0 (palmitic) acid yielded D 31 -18:0 (stearic) acid, D 29 -18:1 (oleic and cis-vaccenic) acids, and D 29 -heptadecene. These findings showed that loss of the carboxyl group of a C 18 monounsaturated fatty acid lead to heptadecene formation. Amount of 7-heptadecene varied with growth phase and temperature and was strictly dependent on light but was not affected by an inhibitor of photosystem II. Cell fractionation showed that approximately 80% of the alkene is localized in the chloroplast. Heptadecane, pentadecane, as well as 7-and 8-heptadecene were detected in Chlorella variabilis NC64A (Trebouxiophyceae) and several Nannochloropsis species (Eustigmatophyceae). In contrast, Ostreococcus tauri (Mamiellophyceae) and the diatom Phaeodactylum tricornutum produced C 21 hexaene, without detectable C 15 -C 19 hydrocarbons. Interestingly, no homologs of known hydrocarbon biosynthesis genes were found in the Nannochloropsis, Chlorella, or Chlamydomonas genomes. This work thus demonstrates that microalgae have the ability to convert C 16 and C 18 fatty acids into alka(e)nes by a new, lightdependent pathway.

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“…were cultivated in 300 mL conical flask of 150 mL culture medium. The culture medium was prepared from Nitrate, phosphate, vitamins and trace elements in natural, filtered (\20 lm) autoclaved seawater according to f/2 medium (Lananan et al 2013). The seawater was sourced from the China Sea near Qidong county, where salinity is approximately 30 psu.…”

Section: Methodsmentioning

confidence: 99%

The Effects of Petroleum Hydrocarbons on Algae Can Be Reversed in the Presence of a Primary Consumer

Wang

Yong

Liu

et al. 2015

Bull Environ Contam Toxicol

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The ecotoxicological effects of a mixture of petroleum hydrocarbons were tested on densities of two algae, Platymonas helgolandica var. tsingtaoensis and Isochrysis galbana, and of a rotifer, Brachionus plicatilis, by single-species and customized community experiments. Test concentrations ranged from 0 to 100 mg L(-1), while five to seven treatments were assessed in triplicate within 1 month. A significant decrease in densities during single-species toxicity tests were found when concentrations of petroleum hydrocarbons were above 1.0 mg L(-1). However, equilibrium densities of algae in the customized community showed a different pattern, which increased with concentration and reached a peak at 20.0 mg L(-1). The community-based no observed effect concentration (NOEC; 1.0 mg L(-1)) was different from the NOEC derived by single-species toxic tests (0.25 mg L(-1)). This demonstrates that ecotoxicological effects on plankton as part of a community is significantly different from single-species toxicity tests owing to ecological interactions.

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Effect of Conway Medium and f/2 Medium on the growth of six genera of South China Sea marine microalgae

Cited by 79 publications

References 30 publications

Growth kinetics and fucoxanthin production of Phaeodactylum tricornutum and Isochrysis galbana cultures at different light and agitation conditions

Growth kinetics and fucoxanthin production of Phaeodactylum tricornutum and Isochrysis galbana cultures at different light and agitation conditions

Microalgae Synthesize Hydrocarbons from Long-Chain Fatty Acids via a Light-Dependent Pathway

The Effects of Petroleum Hydrocarbons on Algae Can Be Reversed in the Presence of a Primary Consumer

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