Effect of convection on osteoblastic cell growth and function in biodegradable polymer foam scaffolds

Goldstein, Aaron S.; Juarez, Tiffany; Helmke, Christopher D; Gustin, Michael C.; Mikos, Antonios G.

doi:10.1016/s0142-9612(00)00280-5

Cited by 431 publications

(417 citation statements)

References 34 publications

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“…In this setting, the neovascular bed originates from the periphery of the construct, which should be implanted into a site of high vascularization potential (Kneser et al, 2006). Since oxygen and nutrition supply by diffusion is restricted to a maximum range of 200 µm into a given matrix (Goldstein et al, 2001), suboptimal initial vascularization often limits the survival of cells in the centre of large constructs. Although generation of new tissue is feasible using these techniques, the newly formed tissue is vascularized in a random pattern, making a transfer to distant implantation sites impossible without destruction of the vascular network.…”

Section: Discussionmentioning

confidence: 99%

Axial vascularization of a large volume calcium phosphate ceramic bone substitute in the sheep AV loop model

Beier¹,

Horch²,

Heß

et al. 2010

J Tissue Eng Regen Med

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Vascularization still remains an obstacle to engineering of bone tissue with clinically relevant dimensions. Our aim was to induce axial vascularization in a large volume of a clinically approved biphasic calcium phosphate ceramic by transferring the arteriovenous (AV) loop approach to a large animal model. HA/β-TCP granula were mixed with fibrin gel for a total volume of 16 cm 3 , followed by incorporation into an isolation chamber together with an AV loop. The chambers were implanted into the groins of merino sheep and the development of vascularization was monitored by sequential non-invasive magnetic resonance imaging (MRI). The chambers were explanted after 6 and 12 weeks, the pedicle was perfused with contrast agent and specimens were subjected to micro-computed tomography (µ-CT) scan and histological analysis. Sequential MRI demonstrated a significantly increased perfusion in the HA/β-TCP matrices over time. Micro-CT scans and histology confirmed successful axial vascularization of HA/β-TCP constructs. This study demonstrates, for the first time, successful axial vascularization of a clinically approved bone substitute with a significant volume in a large animal model by means of a microsurgically created AV loop, thus paving the way for the first microsurgical transplantation of a tissue-engineered, axially vascularized bone with clinically relevant dimensions.

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Section: Discussionmentioning

confidence: 99%

Axial vascularization of a large volume calcium phosphate ceramic bone substitute in the sheep AV loop model

Beier¹,

Horch²,

Heß

et al. 2010

J Tissue Eng Regen Med

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“…Dermal fibroblasts can be easily isolated by biopsy of small fragments of the patient's own skin and utilized for up to 14 subcultures without any decrease in collagen biosynthesis or growth level 5,6 . The ability of cells from the fibroblast lineage to undergo differentiation into the more specialized phenotypes of connective tissue, such as osteoblasts, was first suggested by Urist 9 .…”

Section: Introductionmentioning

confidence: 99%

TGF-β1 on induced osteogenic differentiation of human dermal fibroblast

et al. 2014

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PURPOSE:To evaluate the role of transforming growth factor beta 1 (TGF-β1) on the induced osteogenic differentiation of human dermal fibroblasts. METHODS:We performed four groups with cultured dermal fibroblasts according to the culture medium: CONTROL (DMEM culture medium); TGF-β1 (DMEM culture medium with 10 ng/ml of TGF-β1); OSTEOG (DMEM culture medium with 0.5 µg/ml of ascorbic acid, 10 mmol/l of β-glycerophosphate and 10 nmol/L of dexamethasone); and OSTEOG/TGF-β1 (osteogenic medium with 10 ng/ml of TGF-β1). Alkaline phosphatase (ALP) activity and the amount of osteocalcin (OC) in the supernatant, as well as the capability to form calcium phosphate deposits, were analysed for 28 days RESULTS: There were significant differences (p<0.05) between CONTROL and TGF-β1 groups in comparison with OSTEOG and OSTEOG/TGF-β1 groups in the ALP activity and OC amount. Although, both osteogenic groups had the same behavior with regard the expression curve during the experimental time, the OSTEOG/TGF-β1 group achieved significantly higher ALP and OC levels and showed no significant difference in the levels of mineralized deposits and in comparison with the levels found in the OSTEOG group. CONCLUSION:The addition of transforming growth factor beta 1 to the osteogenic culture medium increased the activity of alkaline phosphatase and the amount of osteocalcin, but TGF-β1 did not alter the presence of mineralized calcium phosphate deposits.

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“…In addition, under 2D static condition, cells cannot distribute uniformly. Bone-inductive medium merely transfers onto the surface of the scaffold on account of the concentration gradient of the nutrients and metabolic products inside the scaffold, while the problems will be sharper if the size of the scaffold become bigger (Goldstein et al 2001). Hence, an appropriate bioreactor is necessary to provide a better environment for fabricating the engineered tissues.…”

Section: Introductionmentioning

confidence: 99%

Repair of bone defects using dynamic fabricated tissue-engineered bone based on a bio-derived porous bone scaffold

Song

et al. 2016

Animal Cells and Systems

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Fabrication and transplantation of tissue-engineered bones in a rotating wall vessel bioreactor (RWVB) was studied in the present study aiming to repair segmental bone defects. Osteoblasts were transfected with green fluorescent protein prior to seeding on bio-derived porous bone scaffolds at a density of 1 × 10 6 cells/mL and cultured in an RWVB for one week. For comparison, constructs were also cultured in a static condition. Morphology and structure of fabricated bones were examined using an inverted microscope, scanning electron microscope and histology analysis via hematoxylin-eosin and toluidine blue staining. Moreover, an animal model for repairing segmental bone defects of a Zelanian rabbit was used to assess the efficacy and biosafety of fabricated bones. In conclusion, tissue-engineered bones grew favorably in RWVB. In animal study, a preliminary repair of bone defects was noticed only in the experimental group after 4 weeks of implantation. Using RWVB, the fabricated tissue-engineered bone constructs were approved with better bio-capability in repairing the segmental bone defect. ARTICLE HISTORY

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Effect of convection on osteoblastic cell growth and function in biodegradable polymer foam scaffolds

Cited by 431 publications

References 34 publications

Axial vascularization of a large volume calcium phosphate ceramic bone substitute in the sheep AV loop model

Axial vascularization of a large volume calcium phosphate ceramic bone substitute in the sheep AV loop model

TGF-β1 on induced osteogenic differentiation of human dermal fibroblast

Repair of bone defects using dynamic fabricated tissue-engineered bone based on a bio-derived porous bone scaffold

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