Effect of constitutive expression of ARGOS-LIKE gene on sizes of cells and organs of transgenic tobacco plants

Kuluev, B. R.; Knyazev, A. V.; Safiullina, M. G.; Чемерис, А. В.

doi:10.1134/s1022795413040078

Cited by 8 publications

(3 citation statements)

References 13 publications

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“…It means that new leaf primordia appear in the places with local weakening of cell walls, which can be caused not only by expansins, but also xyloglucan endotransglucosylases (Miedes et al 2010). In literature there is information about ARGOS gene homologs overexpression being able to induce expression of both expansins (Wang et al 2009a, Kuluev et al 2013) and xyloglucan endotransglucosylases (Hu et al 2006, Kuluev et al 2014. So it can be suggested that overexpression of PnARGOS-LIKE gene also induces the expression of certain proteins, influencing the weakening of the cell wall.…”

Section: Resultsmentioning

confidence: 95%

“…The original tobacco plant line, Petit Havana SR1, was used as a control. We did not use transgenic plants containing T-DNA of pCambia 1301 plasmid without target gene as the control, since these plants did not differ from wild type (Kuluev et al 2013). Control and transgenic seedlings of T 2 generation were germinated on MS medium without phytohormones for 20 d, then the length of roots and hypocotyls were measured and plants were acclimatized to ex vitro conditions.…”

Section: Growth Conditions and Treatments Of Wild Type Aspen Plantsmentioning

confidence: 99%

“…Transgenic vector was constructed by cloning 35S cassette into the binary vector pCambia 1301 (CAMBIA, Australia). The expression cassette containing 35S promoter, polylinker, and cauliflower mosaic virus polyadenylation signal was cut out by restriction enzyme EcoRV from 35S plasmid and cloned to pCambia 1301 by site SmaI (Kuluev et al 2013). PnARGOS-LIKE gene was cloned into the resulting binary vector.…”

Section: Plasmid Construction and Computer Analysismentioning

confidence: 99%

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The poplar ARGOS-LIKE gene promotes leaf initiation and cell expansion, and controls organ size

Kuluev¹,

Knyazev²,

Mikhaylova³

et al. 2016

Biol. plant.

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We identified Populus nigra auxin-regulated gene involved in organ size (PnARGOS)-LIKE encoding one of organ size related (OSR)-proteins in black poplar which is homologous to AtARGOS and AtARGOS-LIKE genes of Arabidopsis thaliana. ABRE-like, G-box, GATA and Ibox motifs were discovered in the promoter region of poplar ARGOS-LIKE gene. In wild type aspen (Populus tremula) plants, ortholog of PnARGOS-LIKE gene (PtrARGOS-LIKE) was noticeably expressed in the actively dividing and expanding young leaves and calli, whereas its mRNA content increased in response to exogenous 6-benzylaminopurine, 1-naphthaleneacetic acid, and 24-epibrassinolide. The expression of PtrARGOS-LIKE gene was reduced under salinity treatment. In addition, we generated transgenic tobacco and aspen plants with up-regulated expression of PnARGOS-LIKE gene. Constitutive expression of the gene contributed to an increase in size of stems and leaves of transgenic tobacco plants. In transgenic aspen constitutive expression of PnARGOS-LIKE gene promoted an increase in the frequency of leaf initiation and in the leaf length and area. The size of transgenic tobacco and aspen leaves increased due to the enlargement of individual cells. The results show the significance of PnARGOS-LIKE gene for the control of leaf initiation and organ growth by cell expansion in poplar.

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