Effect of conjugated linoleic acid on bovine mammary cell growth, apoptosis and stearoyl Co-A desaturase gene expression

Keating, Aileen F.; Zhao, Feng‐Qi; Finucane, Kiera A.; Glimm, David R.; Kennelly, J. J.

doi:10.1016/j.domaniend.2007.08.005

Cited by 19 publications

(22 citation statements)

References 30 publications

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“…The in vitro data of Keating et al [28], however, may help to clarify the effect of relatively high doses of fatty acids in vivo on the mammary gland. Eventually these investigations could be extended to evaluate the effects of CLA on differentiated mammary gland cells in vitro, such as the bovine mammary epithelial cell collagen cell culture system [38].…”

Section: Discussionmentioning

confidence: 92%

“…The effects of other isomers of CLA were not investigated. Our studies of lipid accumulation in MAC-T cells were based on the methods used in the recent report by Keating et al [28] to study bovine mammary cell growth, apoptosis, and stearoyl-CoA (SCD) gene expression. In this recent report, various concentrations of CLA and other FA were applied to MAC-T-cells for 48 h. CLA (cis-9, trans-11 18:2 and trans-10, cis-12 18:2) concentrations of 30 lM or greater resulted in MAC-T-cell apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…Fatty acyl-CoA was prepared from the above FA as described by Taylor [27] and were kindly provided by Nicholas Lemee of Nexia Biotechnologies, Inc. (Montreal, Quebec, Canada). Cells were maintained essentially as described by Keating et al [28]. The cells were cultured in high glucose (4.5 g/l) DMEM containing L-glutamine, sodium pyruvate, and pyroxidine-HCl and supplemented with 10% (v/v) FBS and 5 lg bovine insulin/ml.…”

Section: Chemicalsmentioning

confidence: 99%

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Effect of CLA and Other C18 Unsaturated Fatty Acids on DGAT in Bovine Milk Fat Biosynthetic Systems

Sorensen

Kazala

Murdoch

et al. 2008

Lipids

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Production of dairy products with increased amounts of nutraceutic FA such as conjugated linoleic acid (CLA) represents a recent approach for dairy producers and processors to increase the value of their products. The effect of CLA and other FA on the expression of diacylglycerol acyltransferase-1 (DGAT-1) and DGAT-2, and DGAT activity were investigated in bovine mammary gland epithelial (MAC-T) cells. DGAT gene expression analyses were also conducted using bovine mammary gland tissue from dairy cows. In the studies with MAC-T cells, there were no significant effects of CLA isomers or other FA on DGAT1 expression, whereas all FA tested showed enhanced DGAT2 expression (P < 0.05 to P < 0.001), with alpha-linolenic acid (alpha-18:3) having the greatest effect. Additionally, DGAT2 expression was co-ordinated with expression of lysophosphatidic acid acyltransferase (LPAAT), an observation that was also apparent in mammary gland from lactating dairy cows. In contrast, treatment of MAC-T cells with trans-10, cis-12 18:2 or alpha-18:3 resulted in a significant (P < 0.05) decrease in overall DGAT enzyme activity, although the mechanisms resulting in these effects are unclear. Competition assays using microsomes from bovine mammary gland tissue and 1-[(14)C]oleoyl-CoA suggested that DGAT activity was more selective for oleoyl (cis-9 18:1)-CoA than cis-9, trans-11 18:2-, trans-10, cis-12 18:2- or cis-9, cis-12 18:2-CoA. Collectively, the results suggest the relationship between trans-10, cis-12 18:2 and reduced TAG production in bovine milk is not linked to the production of DGAT1 or DGAT2 transcripts, but probably involves effects of this CLA isomer at events beyond transcription, such as post-translational and/or enzyme activity effects.

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Chemicalsmentioning

confidence: 99%

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Effect of CLA and Other C18 Unsaturated Fatty Acids on DGAT in Bovine Milk Fat Biosynthetic Systems

Sorensen

Kazala

Murdoch

et al. 2008

Lipids

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“…Although one incident of dry-off is insufficient to lead to conclusions, the result is not inconsistent with the speculation of Bell and Kennelly (2003), based on changes in milk osmolyte concentrations and a fivefold elevation in somatic cell count, that the 38% decline in milk yield observed with 150 g d (1 trans-10, cis-12) was due to induction of mammary involution. Indeed, the anticarcinogenic properties of CLA are thought to be due, in large part, to the inhibition of cell proliferation and induction of apoptosis in many tissues, including the mammary glands (Belury 2002;Wahle and Heys 2002;Keating et al 2008).…”

Section: Methodsmentioning

confidence: 99%

Milk production responses of primiparous and multiparous dairy cows to dose of conjugated linoleic acid consumed in rumen inert form

Sippel¹,

Spratt²,

Cant³

2009

Can. J. Anim. Sci.

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Conjugated linoleic acid (CLA) has the potential to be used as a dietary means of manipulating milk fat production of dairy cows to meet specified short-term targets, as in a supply-managed industry. An experiment was conducted to evaluate the dose-response to calcium salts of CLA fed as a top-dress on a corn- and alfalfa-based TMR. In two 5 × 5 Latin squares of 28-d periods, five primiparous and five multiparous cows were fed 0, 22, 45, 67 or 178 g d-1 CLA (29% trans-10, cis-12) as a calcium salt. Results from one multiparous cow on the 178 g d-1 treatment were removed from statistical analysis because milk production fell to 1.2 kg d-1 by the end of the period. On average, milk, lactose and protein yields increased at the lower doses of CLA and decreased to control levels at the highest dose. Milk fat yield declined progressively from 876 to 770, 689, 676 and 543 g d-1 on the five doses. Net energy balance of cows increased linearly from -1.5 Mcal d-1 on the control to 2.0 Mcal d-1 at 178 g d-1 CLA. The percentage depression in milk fat yield at different doses of trans-10, cis-12 CLA was described by the equation y = 54/(1 + 22.8/dose). Comparison with published literature suggested that feeding calcium salts of CLA was 30% as effective in depressing milk fat production as an abomasal infusion, due to rumen biohydrogenation and intestinal indigestibility.Key words: Milk composition, cattle, diet

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“…A number of bovine mammary cell lines have been established, among these the MAC-T (Huynh et al, 1991) and the BME-UV1 (Zavizion et al, 1996) cell lines, both of which were immortalised by transfection with the SV40 T-antigen. MAC-T cells have been used for studies of many bioactives, including fatty acids, growth factors, steroids, retinoids, cytokines and mammary extracts, and parameters such as viability, proliferation, apoptosis, gene expression, epithelial transport, cell signalling and lipogenesis (Woodward et al, 1996;Cohick and Turner, 1998;Berry et al, 2003;Peterson et al, 2004;Zarzynska et al, 2005;Thorn et al, 2006 andBruzelius et al, 2008;Keating et al, 2008;Sorensen et al, 2008;Zhou et al, 2008). MAC-T cells also represent a more general model of the mammary gland, as these cells retain a number of biochemical and morphological characteristics typical of mammary epithelial cells in vivo.…”

Section: Cell-based Models Of the Mammary Glandmentioning

confidence: 99%

Cell-based models to test the effects of milk-derived bioactives

Purup

Nielsen²

2012

Animal

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The life science industries have a strong interest in screening for novel bioactives in complex mixtures like milk and dairy products. Food bioactives are not only important for public health in general, but also have potential therapeutic applications for the treatment of a number of diseases. To identify these novel bioactives, establishment of robust screening assays is essential. The use of in vitro cell-based models for screening and testing have the advantage that several concentrations of mixtures or specific compounds can be assayed at the same time in cells from specific tissues. Primary cell cultures from target organs or established cell lines can be used to identify the most sensitive cells. In addition, a large number of transfected cell lines with very specific sensitivities have been developed. Different endpoints inherent to basal or more sophisticated cellular functions can be investigated, such as cell viability, apoptosis, migration, intracellular signalling, regulation of gene expression, morphology and metabolic alterations. The gastrointestinal tract is an obvious target for bioactive molecules delivered through milk and dairy products, because it lies at the interface between dietary components in the lumen and the internal processes of the host. Identification of bioactive factors that affects proliferation or migration of epithelial cells may have potential applications in promoting gastrointestinal health in both humans and animals. The mammary gland is another target organ of considerable interest since it has been estimated that up to 50% of all newly diagnosed breast cancers may be related to dietary factors. A large number of gastrointestinal and mammary epithelial cell lines are commercially available, but in order to study some cellular functions, primary cultures of freshly isolated cells are often preferred, as established cell lines do not always express specialised properties in culture.

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Effect of conjugated linoleic acid on bovine mammary cell growth, apoptosis and stearoyl Co-A desaturase gene expression

Cited by 19 publications

References 30 publications

Effect of CLA and Other C18 Unsaturated Fatty Acids on DGAT in Bovine Milk Fat Biosynthetic Systems

Effect of CLA and Other C18 Unsaturated Fatty Acids on DGAT in Bovine Milk Fat Biosynthetic Systems

Milk production responses of primiparous and multiparous dairy cows to dose of conjugated linoleic acid consumed in rumen inert form

Cell-based models to test the effects of milk-derived bioactives

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