Effect of compressive force on human osteoblast-like cells and bone remodelling: An in vitro study

Tripuwabhrut, Kanich; Mustafa, Manal; Gjerde, Cecilie; Brudvik, Pongsri; Mustafa, Kamal

doi:10.1016/j.archoralbio.2013.01.004

Cited by 44 publications

(64 citation statements)

References 162 publications

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“…Briefly, confluent cells were subjected to a static compressive force by placing a glass cylinder with lead weights onto the cells of a 6-well plate. The intensity of the force was controlled at 294 Pa by numbers of lead granules placed in the cylinder, because force of compression in vivo is assumed between 2.0 g/cm 2 (196 Pa) to 4.0 g/cm 2 (392 Pa) (10,11). A cell area in contact with the cylinder was marked beforehand on the bottom of the wells.…”

Section: Application Of Compressive Forcementioning

confidence: 99%

MiR-494-3p induced by compressive force inhibits cell proliferation in MC3T3-E1 cells

Iwawaki

Mizusawa

Iwata

et al. 2015

Journal of Bioscience and Bioengineering

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Section: Application Of Compressive Forcementioning

confidence: 99%

MiR-494-3p induced by compressive force inhibits cell proliferation in MC3T3-E1 cells

Iwawaki

Mizusawa

Iwata

et al. 2015

Journal of Bioscience and Bioengineering

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“…In studies that reported contradictory results using similar loading methods, magnitude was a major difference. A previous study reported that a compressive stress of 1.0 g/cm 2 is optimal for osteoblastic differentiation13, but another study observed enhanced osteoblastic differentiation when compression was raised from 2.0 g/cm 2 to 4.0 g/cm 2 11. Therefore, magnitude may be one of the parameters that determine the role of mechanical stress in bone remodeling.…”

mentioning

confidence: 99%

“…Unfortunately, in vitro studies showed contradictory results on the responses under compressive stress: inhibited/enhanced osteoblastic differentiation and inhibited/enhanced osteoblast-regulated osteoclastic differentiation have both been reported9101112. In studies that reported contradictory results using similar loading methods, magnitude was a major difference.…”

mentioning

confidence: 99%

Magnitude-dependent response of osteoblasts regulated by compressive stress

Shen

Geng

Liu

et al. 2017

Sci Rep

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The present study aimed to investigate the role of magnitude in adaptive response of osteoblasts exposed to compressive stress. Murine primary osteoblasts and MC3T3-E1 cells were exposed to compressive stress (0, 1, 2, 3, 4, and 5 g/cm2) in 3D culture. Cell viability was evaluated, and expression levels of Runx2, Alp, Ocn, Rankl, and Opg were examined. ALP activity in osteoblasts and TRAP activity in RAW264.7 cells co-cultured with MC3T3-E1 cells were assayed. Results showed that compressive stress within 5.0 g/cm2 did not influence cell viability. Both osteoblastic and osteoblast-regulated osteoclastic differentiation were enhanced at 2 g/cm2. An increase in stress above 2 g/cm2 did not enhance osteoblastic differentiation further but significantly inhibited osteoblast-regualted osteoclastic differentiation. This study suggested that compressive stress regulates osteoblastic and osteoclastic differentiation through osteoblasts in a magnitude-dependent manner.

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“…It was demonstrated that OPN has a role in cell adhesion, migration, cell survival and bone remodelling [18][19][20]. Research in animal models has indicated that OPN deficiency alters the functionality of multiple cell types, resulting in delayed early vascularization, altered matrix organization and late bone remodelling [21].…”

Section: Discussionmentioning

confidence: 99%

Osteopontin, osteocalcin, and osteoprotegerin expression in human tissue affected by cleft lip and palate

Smane

Pilmane

2016

SHS Web Conf.

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Abstract. Cleft lip and palate (CLP) is a common congenital anomaly with a complex etiology which has not been elucidated yet. This study investigated whether expression of osteopontin (OPN), osteoprotegerin (OPG), and osteocalcin (OC), which are essential for the normal craniofacial bone remodelling, is not regulated in children with CLP. Alveolar bone tissue samples were obtained from patients with complete bilateral (CB) CLP (n = 14) during corrective plastic surgery and unaffected control subjects (n = 9). OPN, OPG, and OC expression was assessed by immunohistochemistry, and data were analyzed with the Mann-Whitney test. OPN expression was observed only sporadically in the alveolar bone of 3 patients, in contrast to the control group (z = − 2.962; P < 0.003). The number of OPG-positive bone cells varied from occasional to moderate, in contrast to the control group (z = − 2.247; P = 0.025). OC-positive osteocytes were present in moderate to numerous numbers in both patients and controls, with no significant difference between them (z = − 1.356; P < 0.175). The prominent expression of OC characteristic for CBCLP affected hard tissue indicates a high potential of bone mineralization. Few OPG-positive osteocytes in the bone tissue implicate the disregulation of osteoclast differentiation, maturation, and activity, but few OPN-containing cells may prove the common disregulation of bone remodelling during cleft morphopathogenesis.

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Effect of compressive force on human osteoblast-like cells and bone remodelling: An in vitro study

Abstract: The results suggest that initial application of CF on HOBs can simultaneously affect expression of markers related to both osteogenesis and osteoclastogenesis.

Cited by 44 publications

References 162 publications

MiR-494-3p induced by compressive force inhibits cell proliferation in MC3T3-E1 cells

MiR-494-3p induced by compressive force inhibits cell proliferation in MC3T3-E1 cells

Magnitude-dependent response of osteoblasts regulated by compressive stress

Osteopontin, osteocalcin, and osteoprotegerin expression in human tissue affected by cleft lip and palate

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