Effect of combined vitamins C and E supplementation on oxidant/antioxidant status in hemodialysis patients

Abstract

The aim of this study was to evaluate the antioxidant supplementation effect on antioxidant system in hemodialysis patients. The study group consisted of 38 patients undergoing hemodialysis and 72 healthy subjects. The patients orally received combination of vitamins C (250 mg/time) and E (200 IU/time) three times per week for 2 months. Both, enzymatic antioxidants [superoxide dismutase (SOD) and glutathione peroxidase (GPx)] activities and non-enzymatic (vitamins E and C) levels and malondialdehyde (MDA) concentrations as lipid peroxidation product in patients were measured before and after supplementation compared with control group. The levels of antioxidants (vitamin E, C and SOD) (P \ 0.0001) and GPx (P \ 0.05) were significantly enhanced and MDA concentrations declined (P \ 0.0001) during the supplementation period. The study demonstrates that combined supplementation of vitamins C and E can noticeably affect antioxidant status and protect against oxidative stress caused during hemodialysis.

Effect of peritoneal dialysis on antioxidant defense system and oxidative stress

“…14,15 Erythrocyte SOD and GPx activity were measured using commercial colorimetric assay kit (Randox, Crumlin, County Antrim, UK) as described previously. 11,15 Serum vitamin A and E were extracted with hexane and measured by using high pressure liquid chromatography with absorption at 325 nm and 292 nm, respectively. 11,15 Plasma total vitamin C concentration was measured spectrophotometerically by the 2,4 dinitrophenylhydrazine method.…”

“…11,15 Serum vitamin A and E were extracted with hexane and measured by using high pressure liquid chromatography with absorption at 325 nm and 292 nm, respectively. 11,15 Plasma total vitamin C concentration was measured spectrophotometerically by the 2,4 dinitrophenylhydrazine method. 11,15 Plasma MDA level was assayed as the end product of lipid proxidation by thiobarbituric acid method at 530 nm.…”

“…11,15 Plasma total vitamin C concentration was measured spectrophotometerically by the 2,4 dinitrophenylhydrazine method. 11,15 Plasma MDA level was assayed as the end product of lipid proxidation by thiobarbituric acid method at 530 nm. 11,15 Statistical analysis Data were analyzed using SPSS 15.0 for Windows (SPSS, Chicago, IL, USA) and are expressed as mean AE SD.…”

“…11,15 Plasma MDA level was assayed as the end product of lipid proxidation by thiobarbituric acid method at 530 nm. 11,15 Statistical analysis Data were analyzed using SPSS 15.0 for Windows (SPSS, Chicago, IL, USA) and are expressed as mean AE SD. The different variables between PD patients and control subjects were compared using independent samples Student t test.…”

“…2,6 On the other hand, increased oxidative stress has been suggested as one possible mechanism of peritoneum structural and functional changes resulting from prolonged exposure to PD solutions containing high concentrations of glucose, obstruction of catheter and high incidence of peritonitis. 8e10 The most important antioxidants that protect from oxidant damage include: erythrocyte superoxide dismutase (SOD) and glutathione peroxidase (GPx); and nonenzymatic antioxidants vitamins A, C, and E. 2,5,6,11,12 The importance of oxidative stress in the pathogenesis of cardiovascular complications has recently been emphasized. 6,10,12,13 Thus, the present study was undertaken to investigate some antioxidant markers and potential effects PD on oxidative stress.…”