Effect of combination treatment with entomopathogenic fungi Beauveria bassiana and Nomuraea rileyi (Hypocreales) on Spodoptera litura (Lepidoptera: Noctuidaeae)

Rao, C. Uma Maheswara; Devi, K. Uma; Khan, P. Akbar Ali

doi:10.1080/09583150500335632

Cited by 18 publications

(11 citation statements)

References 20 publications

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“…gambiae s.s., neither an additive nor synergistic effect was evident. Similar results have been found when entomopathogens were evaluated in combination against arthropods other than mosquitoes [22-25]. The initial interaction between the two fungal species occurs at the point of mosquito exposure.…”

Section: Discussionsupporting

confidence: 79%

Infection of the malaria mosquito, Anopheles gambiae, with two species of entomopathogenic fungi: effects of concentration, co-formulation, exposure time and persistence

Mnyone

Kirby

Lwetoijera

et al. 2009

Malar J

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BackgroundEntomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana isolates have been shown to infect and reduce the survival of mosquito vectors.MethodsHere four different bioassays were conducted to study the effect of conidia concentration, co-formulation, exposure time and persistence of the isolates M. anisopliae ICIPE-30 and B. bassiana I93-925 on infection and survival rates of female Anopheles gambiae sensu stricto. Test concentrations and exposure times ranged between 1 × 107 - 4 × 1010 conidia m-2 and 15 min - 6 h. In co-formulations, 2 × 1010 conidia m-2 of both fungus isolates were mixed at ratios of 4:1, 2:1, 1:1,1:0, 0:1, 1:2 and 1:4. To determine persistence, mosquitoes were exposed to surfaces treated 1, 14 or 28 d previously, with conidia concentrations of 2 × 109, 2 × 1010 or 4 × 1010.ResultsMosquito survival varied with conidia concentration; 2 × 1010 conidia m-2 was the concentration above which no further reductions in survival were detectable for both isolates of fungus. The survival of mosquitoes exposed to single and co-formulated treatments was similar and no synergistic or additive effects were observed. Mosquitoes were infected within 30 min and longer exposure times did not result in a more rapid killing effect. Fifteen min exposure still achieved considerable mortality rates (100% mortality by 14 d) of mosquitoes, but at lower speed than with 30 min exposure (100% mortality by 9 d). Conidia remained infective up to 28 d post-application but higher concentrations did not increase persistence.ConclusionBoth fungus isolates are effective and persistent at low concentrations and short exposure times.

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Section: Discussionsupporting

confidence: 79%

Infection of the malaria mosquito, Anopheles gambiae, with two species of entomopathogenic fungi: effects of concentration, co-formulation, exposure time and persistence

Mnyone

Kirby

Lwetoijera

et al. 2009

Malar J

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“…Staves and Knell’s [46] findings suggest that if mixed infection persists, then the type of interaction (direct and/or indirect) within the host can play a major role in determining how an entomopathogen will evolve in terms of its virulence. When the combination of two entomopathogenic fungal isolates was tested, all possible effects (additive, synergistic, competitive) were recorded [31,32,33] depending on the host and pathogen species, temperature, and pathogen concentration.…”

Section: Discussionmentioning

confidence: 99%

“…Additive and synergistic effects of entomopathogenic fungi with other insecticidal treatments have been validated in many previous studies, concerning mainly the combined use with entomopathogenic bacteria [14,18,19,20,21,22], viruses [23], nematodes [16,17,24,25,26], synthetic insecticides [27,28,29,30], and insecticidal dust [10,12]. On the contrary, there are very few studies investigating the combined action of two fungal entomopathogens [31,32,33].…”

Section: Introductionmentioning

confidence: 99%

“…Firstly, variable insecticidal metabolites and toxins produced by fungal entomopathogens have several modes of action (some of them remain partly unknown) and, in many cases, they often constitute the direct cause of insect death [34,35]. Secondly, similar entomopathogens may act differently on insects with varying behaviors and in different environments [31,32,36,37].…”

Section: Introductionmentioning

confidence: 99%

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Ιnteractions between Beauveria bassiana and Isaria fumosorosea and Their Hosts Sitophilus granarius (L.) and Sitophilus oryzae (L.) (Coleoptera: Curculionidae)

Mantzoukas

Zikou

Triantafillou

et al. 2019

Insects

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The interactions between the entomopathogenic fungus Beauveria bassiana Balsamo (Vuillemin) (Hypocreales: Cordycipitaceae) and the entomopathogenic fungus Isaria fumosorosea (Wize) Brown and Smith (Hypocreales: Clavicipitaceae) were examined on young adults of Sitophilus granarius (L.) (Coleoptera: Curculionidae) and S. oryzae (L.) (Coleoptera: Curculionidae). Conidial suspensions of these entomopathogenic fungi were applied both separately and in combination, at three dosages, 104, 106, and 108 conidia/mL. Mortality of experimental adults was recorded daily for 15 days. An overall positive interaction between the pathogenic microorganisms was observed. Mean weevil mortality caused by the separate acting fungi, B. bassiana, ranged from 26.7% to 53.3% and from 36.6% to 63.3% for S. granarius and S. oryzae, respectively. The respective values for I. fumosorosea were 20.0%–53.3% and 46.7%–66.7%. The combined treatments showed a distinct interaction between the pathogens; for S. granarius, the interaction between the pathogens was additive in all combinations, whereas, for S. oryzae, the interaction was additive in seven and competitive in two of the combinations. Applying both entomopathogenic microorganisms may offer a method for weevil control that could be more effective than using each pathogen alone.

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“…Under both in vivo and in vitro conditions, unique SCAR markers of both the strains prevailed. On the other hand, synergism appeared not to be evident with respect to the combined effect of B. bassiana strains against Galleria mellonella (Wang et al, 2002), two strains of M. anisopliae against Anopheles gambiae (Mnyone et al, 2009), and Beauveria and Nomuraea rileyi against S. litura larvae (Uma Maheswara Rao et al, 2006). However, in the intergeneric combinations of B55 + M48, B51 + M20, and B51 + M48 in the present study, only one strain predominated under in vivo conditions, but not in the corresponding in vitro experiments.…”

Section: Discussionmentioning

confidence: 99%

Tracking coformulated strains of the entomopathogenic fungi Beauveria bassianaand Metarhizium anisopliae using SCAR markers

Ummidi¹,

Vadlamani²

2016

Turk J Biol

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This article examines the development of strain-specific sequence-characterized amplified region (SCAR) molecular markers in two strains of Beauveria bassiana and Metarhizium anisopliae, as well as their use for tracking pathogens in coinfected insect pests. The markers were designed based on the polymorphic introns of the large subunit region of the ribosomal DNA. These markers were used to distinguish between two strains of Beauveria (B55 and B51) and two strains of Metarhizium (M20 and M48). The entomopathogenic strains demonstrated synergistic increase in mortality against Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) larvae when infected with the coformulated M. anisopliae strain M20 + B. bassiana strain B55, particularly at a 2:1 proportion of LC 50 concentration. The study revealed a disparity between intergeneric and interstrain coformulations. In intergeneric coformulations, one strain appeared predominant over the other strain at 1:2 and 1:4 proportions, both under in vivo and in vitro conditions. On the other hand, in interstrain coformulations, both strains survived and formed heterokaryons. Molecular studies revealed that the heterokaryons were unstable and reverted back to any one of the parent strains after 3 or 4 generations.

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Effect of combination treatment with entomopathogenic fungi Beauveria bassiana and Nomuraea rileyi (Hypocreales) on Spodoptera litura (Lepidoptera: Noctuidaeae)

Cited by 18 publications

References 20 publications

Infection of the malaria mosquito, Anopheles gambiae, with two species of entomopathogenic fungi: effects of concentration, co-formulation, exposure time and persistence

Infection of the malaria mosquito, Anopheles gambiae, with two species of entomopathogenic fungi: effects of concentration, co-formulation, exposure time and persistence

Ιnteractions between Beauveria bassiana and Isaria fumosorosea and Their Hosts Sitophilus granarius (L.) and Sitophilus oryzae (L.) (Coleoptera: Curculionidae)

Tracking coformulated strains of the entomopathogenic fungi Beauveria bassianaand Metarhizium anisopliae using SCAR markers

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