Effect of Combination of Some Phytoconstituents of Pharmacological Interest on Cutaneous Wound Healing in Diabetic Rats

Abstract

Persistent non-healing wounds present a substantial challenge in the context of diabetes management. This research delves into exploring the wound healing capabilities of a combination of natural plant-derived compounds, namely embelin, berberine, ferulic acid, and asiatic acid, in streptozotocin induced diabetic rats. Various wound models, including dead space, incision, and excision models, were employed to evaluate their impact. The phytoconstituents were carefully blended into an ointment formulation, with a concentration of 5% (w/w), specifically designed to cater to their individual strengths. Notably, diabetic rats exhibited a significant enhancement in wound closure and improved skin epithelialization. Furthermore, this combination of phytoconstituents exhibited efficacy when administered orally in both the incision and dead space wound models. In the incision wound model, granulation tissues were examined on the 8th day following the initial injury, and assessments were made for hydroxyproline, hexosamine, total protein. In streptozotocin-induced diabetic rats, both topical and oral applications of this combination revealed a marked increase in hydroxyproline, hexosamine, indicating a favorable impact on wound healing. Additionally, the combination led to a substantial improvement in wound breaking strength. When assessed in the dead space model, the combination notably increased the weight and breaking strength of granuloma tissue. These findings collectively point to the wound healing acceleration attributed to Combination in diabetic rats.