Effect of combination of electrolyte and buffer on electrical production in fuel cell microbial system with<i>Pseudomonas sp.</i>in molasses substrate

Baharuddin, Maswati; Rajib, Muh; Sappewali,; Zahra, Ummi

doi:10.1051/e3sconf/202021103001

Cited by 5 publications

(4 citation statements)

References 10 publications

(9 reference statements)

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“…A stainless steel wire was used to link the electrodes in bioreactors so that electrons could be transferred. A 50 mM phosphate buffer solution was employed in the cathode chamber, which was left open to the atmosphere for ventilation of O 2 as an electron acceptor, while the anode chamber was completely sealed with epoxy sealant to maintain an anaerobic environment [ 42 , 79 ]. The anode substrate was obtained in fed-batch mode using the molasses wastewater (g L −1 ) with 50 mL as the carbon source, 2.0 g yeast extract, 2.0 g peptone, 0.5 g NaCl, 0.1 g KH 2 PO 4 , 1.0 g K 2 HPO 4 , 2.5 g NaNO 3 , 0.5 g MgSO 4 , 0.5 g MgCl 2 , and 4% inoculum size.…”

Section: Methodsmentioning

confidence: 99%

Statistical optimization of waste molasses-based exopolysaccharides and self-sustainable bioelectricity production for dual chamber microbial fuel cell by Bacillus piscis

Sakr,

Khater,

Kheiralla

et al. 2023

Microb Cell Fact

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Background The application of exopolysaccharide-producing bacteria (EPS) in dual chamber microbial fuel cells (DCMFC) is critical which can minimize the chemical oxygen demand (COD) of molasses with bioelectricity production. Hence, our study aimed to evaluate the EPS production by the novel strain Bacillus piscis by using molasses waste. Therefore, statistical modeling was used to optimize the EPS production. Its structure was characterized by UV, FTIR, NMR, and monosaccharides compositions. Eventually, to highlight B. piscis' adaptability in energy applications, bioelectricity production by this organism was studied in the BCMFC fed by an optimized molasses medium. Results B. piscis OK324045 characterized by 16S rRNA is a potent EPS-forming organism and yielded a 6.42-fold increase upon supplementation of molasses (5%), MgSO4 (0.05%), and inoculum size (4%). The novel exopolysaccharide produced by Bacillus sp. (EPS-BP5M) was confirmed by the structural analysis. The findings indicated that the MFC's maximum close circuit voltage (CCV) was 265 mV. The strain enhanced the performance of DCMFC achieving maximum power density (PD) of 31.98 mW m−2, COD removal rate of 90.91%, and color removal of 27.68%. Furthermore, cyclic voltammetry (CV) revealed that anodic biofilms may directly transfer electrons to anodes without the use of external redox mediators. Additionally, CV measurements made at various sweep scan rates to evaluate the kinetic studies showed that the electron charge transfer was irreversible. The SEM images showed the biofilm growth distributed over the electrode’s surface. Conclusions This study offers a novel B. piscis strain for EPS-BP5M production, COD removal, decolorization, and electricity generation of the optimized molasses medium in MFCs. The biosynthesis of EPS-BP5M by a Bacillus piscis strain and its electrochemical activity has never been documented before. The approach adopted will provide significant benefits to sugar industries by generating bioelectricity using molasses as fuel and providing a viable way to improve molasses wastewater treatment.

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Section: Methodsmentioning

confidence: 99%

Statistical optimization of waste molasses-based exopolysaccharides and self-sustainable bioelectricity production for dual chamber microbial fuel cell by Bacillus piscis

Sakr,

Khater,

Kheiralla

et al. 2023

Microb Cell Fact

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“…Figure 4 shows the current strength values produced by the biocathode at various weights. The largest maximum current produced was found in the electrolyte solution, namely 1.30 mA, while research by (Baharuddin et al, 2020), which used Pseudomonas sp. with a molasses substrate with the addition of 0.2 M KMnO4 electrolyte solution and potassium phosphate buffer produced current strength of 1.44 mA.…”

Section: Biocathode Current Strengthmentioning

confidence: 97%

“…One of the sugars contained in molasses in the form of sucrose in molasses drops has a sucrose content that varies around 12-40% and has a reducing sugar content of 12-35% (Rochani et al, 2016). The molasses substrate in the MFC system was also used in research by (Baharuddin et al, 2020) with a combination of electrolyte solutions in the form of KMnO4, K3Fe(CN)6 as well as sodium phosphate buffer and potassium phosphate buffer. This research resulted in a maximum voltage value of 0.67 volts, a current strength of 1.43 mA, and a maximum power density of 660.82 mW.m -2 in the KMnO4 electrolyte solution with a combination of sodium phosphate buffer solution.…”

Section: Introductionmentioning

confidence: 99%

The Ability of the Raccoon Tail Water Plant (Ceratophyllum demersum) as a Biocathode in the Plant-Microbial Fuel Cell System

Saharuddin,

Febryanti,

Azis

2024

JSAL

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Plant-Microbial Fuel Cell (P-MFC) is a green technology because it uses a biocathode in the cathode compartment and also uses microorganisms to break down the chemical energy of organic matter into electrical energy. In this research, molasses and Saccharomyces cereviceae were used as substrates and Ceratophyllum demersum as a biocathode in the cathode compartment. The purpose of this study was to determine the potential variation in plant weight of C. demersum as a biocathode in the P-MFC system. The results of this study indicated that the maximum current at the biocathode was at a weight of 70 g, namely 0.180 mA with a power density value of 13.664 mW.m-2 and the maximum potential difference at the biocathode was at a weight of 40 g, which as 0.310 mV with a power density value of 30.787 mW.m-2. Therefore, coontail water plant has the potential as biocathode.

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“…After that, the graphite electrode was immersed again with sodium hydroxide (NaOH) 1 M for 1 x 24 hours. Next, the graphite electrode was rinsed with distilled aquadest (H2O) until neutral (Baharuddin et al, 2020) Meanwhile, the PEM preparation was carried out by means of a membrane in the form of Nafion 117 heated using aquadest (H2O) at 80˚C for 1 hour, then heated using H2O2 3% 80˚C for 1 hour and rinsed with aquadest (H2O). Furthermore, the Nafion 117 membrane was reheated with H2SO4 1 M solution for 1 hour and washed with aquadest (H2O) 3 times.…”

Section: Electrode and Pem Preparationmentioning

confidence: 99%

Electrical Energy Production with Microbial Fuel Cell (MFC) Technology on Gracilaria verrucosa Substrate

Rusdin¹,

Papriani

2023

Int J Sci Healthcare Res

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Microbial Fuel Cell (MFC) is a renewable technology based on electrochemistry that has the ability to convert chemical energy into electrical energy through microbial metabolic processes. This study aims to determine the effect of adding an electrolyte solution to the production of electricity generated using the substrate Gracilaria verrucosa and the microbe Saccharomyces cerevisiae. The method used in this study uses a double chamber consisting of anode and cathode chambers connected through a proton exchange membrane (PEM) in the form of Nafion 117. The results showed that the addition of 0.2 M KMnO4 electrolyte solution produced current, voltage and power density respectively. of 0.74 mA; 580 mV and 2187 mW/cm2 higher than the addition of 0.2 M K3Fe(CN)6 electrolyte solution resulted in a current, voltage and power density of 0.69 mA, respectively; 450 mV and 1582.16 mW/cm2. The MFC system using Gracilaria verrucosa as a substrate has the potential to generate electrical energy. Keywords: Microbial Fuel Cell (MFC), Electrical energy, Gracilaria verrucosa, Saccharomyces cerevisiae

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Effect of combination of electrolyte and buffer on electrical production in fuel cell microbial system withPseudomonas sp.in molasses substrate

Cited by 5 publications

References 10 publications

Statistical optimization of waste molasses-based exopolysaccharides and self-sustainable bioelectricity production for dual chamber microbial fuel cell by Bacillus piscis

Statistical optimization of waste molasses-based exopolysaccharides and self-sustainable bioelectricity production for dual chamber microbial fuel cell by Bacillus piscis

The Ability of the Raccoon Tail Water Plant (Ceratophyllum demersum) as a Biocathode in the Plant-Microbial Fuel Cell System

Electrical Energy Production with Microbial Fuel Cell (MFC) Technology on Gracilaria verrucosa Substrate

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