Effect of combination of curcumin and <scp>GLUT</scp>‐1 <scp>AS‐ODN</scp> on radiosensitivity of laryngeal carcinoma through regulating autophagy

Dai, Li‐Bo; Qi, Yu; Zhou, Shui‐Hong; Bao, Yang‐Yang; Zhong, Jiang-Tao; Shen, Lianfang; Zhang, Lu; Fan, Jun; Huang, Ya‐Ping

doi:10.1002/hed.26180

Cited by 13 publications

(13 citation statements)

References 47 publications

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“…As reported by some previous studies [20,21], there is also high expression of GLUT-1 in laryngocarcinoma, and the expression of GLUT-1 is related to the radioresistance of laryngocarcinoma. Thus, inhibiting the expression of GLUT-1 can improve the radiosensitivity of laryngocarcinoma.…”

Section: Introductionsupporting

confidence: 67%

Chitosan-Based Glycolipid Conjugated siRNA Delivery System for Improving Radiosensitivity of Laryngocarcinoma

et al. 2021

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Glucose Transporter-1 (GLUT-1) is considered to be a possible intrinsic marker of hypoxia in malignant tumors, which is an important factor in radioresistance of laryngocarcinoma. We speculated that the inhibition of GLUT-1 expression might improve the radiosensitivity of laryngocarcinoma. GLUT-1 siRNA was designed to inhibit the GLUT-1 expression, but the high molecular weight and difficult drug delivery limited the application. Herein, we constructed a glycolipid polymer chitosan oligosaccharide grafted stearic acid (CSSA) to conjugate siRNA via electrostatic interaction. The characteristics of CSSA and CSSA/siRNA were studied, as well as the radiosensitization effect of siRNA on human laryngocarcinoma epithelial (Hep-2) cells. Compared with the traditional commercial vector LipofectamineTM2000 (Lipo), CSSA exhibited lower cytotoxicity, more efficiently cellular uptake. Incubating with CSSA/siRNA, the survival rates of Hep-2 cells were significantly decreased comparing with either the group before transfection or Lipo/siRNA. CSSA is a promising carrier for efficient siRNA delivery and radiosensitization of laryngocarcinoma.

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Section: Introductionsupporting

confidence: 67%

Chitosan-Based Glycolipid Conjugated siRNA Delivery System for Improving Radiosensitivity of Laryngocarcinoma

et al. 2021

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“…Glut-1 modulates the Warburg effect by regulating glucose uptake into tumor tissues. We have reported that expression of Glut-1 in laryngeal carcinoma is elevated [ 27 ] and was associated with the proliferation and radioresistance of laryngeal carcinoma cells; targeted inhibition of Glut-1 expression suppressed their proliferation and radioresistance [ 26 – 28 ]. Although expression of Glut-1 was lower in HVCLCs than in laryngeal carcinoma Tu212 and AMC-HN-8 cells, we detected Glut-1 expression in laryngeal precancerous lesions.…”

Section: Discussionmentioning

confidence: 99%

“…Glucose metabolism involves a metabolic shift from oxidative phosphorylation (OXPHOS) to glycolysis [24,25]. Glucose transporter-1 (Glut-1) is an important regulator of energy demand during the development of precancerous lesion cells [26,27]. High expression of Glut-1 enhanced glycolysis, increasing lactate28 and reducing the microenvironment pH.…”

Section: Introductionmentioning

confidence: 99%

Role and mechanism of Glut-1 and H+/K+-ATPase expression in pepsin-induced development of vocal cord leukoplakia

Cao

et al. 2021

Eur Arch Otorhinolaryngol

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Purpose We investigated the role of Glut-1 and H+/K+-ATPase expression in pepsin-induced development of human vocal cord leukoplakia cells (HVCLCs). Next, we analyzed the relationship between Glut-1 and H+/K+-ATPase expression with the clinicopathological features of laryngeal carcinoma. Methods Glut-1 and H+/K+-ATPase expression levels in HVCLCs were determined after treatment with artificial gastric juice containing pepsin and laryngeal carcinoma tissues. Results Exposure to pepsin-containing artificial gastric juice significantly enhanced the migration and proliferation of VSCLCs in a time-dependent manner. The apoptotic rate of VSCLCs decreased over time after exposure to pepsin and reached a nadir on day 7 (p < 0.01). With increasing duration of exposure to pepsin, the proportion of VSCLCs in G0/G1 phase decreased and the proportions in the S and G2/M phases significantly increased (p < 0.05). After treatment with pepsin-containing artificial gastric juice, RT-PCR and Western blotting showed that the expression of Glut-1 and H+/K+-ATPase α, β significantly increased in HVCLCs compared to in the absence of pepsin (p < 0.05). The expression of Glut-1 and H+/K+-ATPase α, β gradually increased from vocal cord leukoplakia (VLC) to laryngeal carcinoma (p < 0.05). Lentivirus-mediated inhibition of Glut-1 expression in VCL significantly inhibited the cells’ migration and proliferation (p < 0.05) but enhanced their apoptosis (p < 0.05). Also, inhibition of Glut-1 expression resulted in an increased proportion of cells in G0/G1 phase and a significantly decreased proportion in G2/M phase (p < 0.05). Conclusions Elevated Glut-1 expression may promote the development of VCL by upregulating laryngeal H+/K+-ATPase expression to reactivate absorbed pepsin, thus damaging the laryngeal mucosa.

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“…Abnormal Beclin 1 expression was associated with chemo-radiotherapy resistance in head and neck cancer. In combination with our previous studies, [125][126][127][128] we will continue to investigate the role of Beclin 1-mediated autophagy in laryngeal carcinoma as well as the role of Beclin1 in glucose transporter-1 (GLUT1)-mediated radioresistance. Furthermore, future studies should be designed to test the effects of Beclin1 activity on GLUT-1 expression as a pathway to improve the radiosensitivity of laryngeal carcinoma.…”

Section: Conclusion and Future Prospectsmentioning

confidence: 90%

<p>Autophagy-Related Beclin 1 and Head and Neck Cancers</p>

Hu¹,

Zhong²,

Gong³

et al. 2020

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Beclin 1, a positive regulator of autophagy, behaves as a double-edged sword in tumorigenesis. Beclin 1 contributes to tumor suppression by removing defective or damaged organelles and other cellular components; however, its activity can also stimulate cancer initiation and progression. In head and neck cancer, Beclin 1 overexpression promotes autophagy, which limits DNA damage and chromosomal instability and increases necrosis and inflammation by impacting apoptotic and autophagic pathways. This paper reviews the relationship between Beclin 1, carcinogenesis and head and neck cancer prognosis.

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Effect of combination of curcumin and GLUT‐1 AS‐ODN on radiosensitivity of laryngeal carcinoma through regulating autophagy

Cited by 13 publications

References 47 publications

Chitosan-Based Glycolipid Conjugated siRNA Delivery System for Improving Radiosensitivity of Laryngocarcinoma

Chitosan-Based Glycolipid Conjugated siRNA Delivery System for Improving Radiosensitivity of Laryngocarcinoma

Role and mechanism of Glut-1 and H+/K+-ATPase expression in pepsin-induced development of vocal cord leukoplakia

<p>Autophagy-Related Beclin 1 and Head and Neck Cancers</p>

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