Effect of Combination from Purple Sweet Potato (Ipomoea batatas L.) Ethanolic Extract and Ramipril Administration on Myocardial Platelet Derived Growth Factor an Expression and Myocardial Collagen Deposition in Hypertensive Rat Models

Abstract

Objective: To determine the effect of administration of combination of purple sweet potato (Ipomoea Batatas L.) ethanolic extract and ramipril in a rat model of hypertension. Methods: This is an experimental study with a post-test only control group design and is a collaborative study of the effect of giving a combination of ethanol extract of purple sweet potato tuber (Ipomoea batatas L.) and ramipril in a rat model of hypertension. Thirty male Wistar rats were given 2 mL/day of 4% NaCl diet to induce hypertension. The rats were divided into 3 treatment groups, consisting of NaCl 4% 2 mL/day+combination therapy with purple sweet potato tuber ethanol extract 400 mg/kgBW/day and ramipril 1 mg/day, NaCl 4% 2 mL/day+purple sweet potato tuber ethanol extract 400 mg/kgBW/day and NaCl 4% 2 mL/day+ramipril 1 mg/day. At week 5, rat was executed to examine myocardial PDGF-A expression and measure myocardial collagen deposition. All the obtained data were analyzed statistically. Results: Administration of ethanolic extract of purple sweet potato 400mg/kgBW/day + ramipril 1mg/day significantly reduced myocardial PDGF-A expression compared to ethanolic extract of purple sweet potato alone (mean difference 14.17 pg/mLCI95% 2.36 – 25.98, P value = 0.016) or ramipril 1mg/day (mean difference 25.58 pg/mL; CI95% 17.07 – 40.09l, P value < 0.001). Administration of ethanolic extract of purple sweet potato 400mg/kgBW/day + ramipril 1mg/day significantly reduced myocardial collagen deposition when compared to administration of purple sweet potato alone (mean difference: 3.30%; CI 95% 1.97 – 4.64; P value <0.001). Administration of ethanolic extract of purple sweet potato combined with ramipril has higher potential effect in reducing myocardial PDGF-A expression and myocardial collagen deposition, compared to single therapy. Conclusion: Administration of ethanolic extract of purple sweet potato combined with ramipril was able to provide the best reduction in myocardial PDGF-A expression and myocardial collagen deposition.