Effect of collagens from jellyfish and bovine Achilles tendon on the activity of J774.1 and mouse peritoneal macrophage cells

Jellyfish collagen stimulates maturation of mouse bone marrow-derived dendritic cells

“…It has also been reported that collagenasetreated jellyfish extract lost its stimulatory effects on immunoglobulin production by HB4C5 cells (Sugahara et al, 2006) and decreased its cytokine production-stimulating activity on murine macrophage-like J774.1 cells (Putra et al, 2014). Moreover, we have also revealed the immunostimulatory effects of commercial bovine Achilles' tendon collagen, which was dissolved by the same method as jellyfish extract, as an example of highly purified collagen (Morishige et al, 2011;Nishimoto et al, 2008;Putra et al, 2012). However, our previous report showed that the active fraction in jellyfish extract mainly contains collagen fragments whose molecular weights were between 15 and 30 kDa (Nishimoto et al, 2008).…”

“…In this study we used jellyfish as a source of substances possessing biological functions. Previous studies have revealed that the extract of jellyfish N. nomurai stimulates immunoglobulin and cytokine production by lymphocytes and macrophages in vitro and in vivo (Morishige et al, 2011;Nishimoto et al, 2008;Putra et al, 2012;Sugahara et al, 2006). It has also been reported that collagenasetreated jellyfish extract lost its stimulatory effects on immunoglobulin production by HB4C5 cells (Sugahara et al, 2006) and decreased its cytokine production-stimulating activity on murine macrophage-like J774.1 cells (Putra et al, 2014).…”

“…The assay was performed as previously described by Putra et al (2012) with some modifications. The enriched BMDCs were seeded into 35 mm culture dishes (BD Falcon) at 1.25 × 10 6 cells/ dish in 10% FBS-RPMI 1640 medium supplemented with 20 ng/ mL rmGM-CSF (R&D Systems) with/without 900 µg/mL of jellyfish collagen or 100 ng/mL of LPS (Sigma-Aldrich) treatment and incubated at 37°C in a CO2 incubator for 24 h. Culture medium containing floating and loosely-adherent cells were collected and centrifuged.…”

“…Moreover, the immunoregulatory function of jellyfish collagen has been highlighted in vivo, resulting in the enhancement of the antigen-specific immune response (Morishige et al, 2011). Jellyfish collagen also highly stimulated production of tumour necrosis factor (TNF)-α and interleukin (IL)-6 by macrophages (Putra et al, 2012), through activation of NF-κB and c-Jun N-terminal kinase via the Tolllike receptor 4 signalling pathway (Putra, Nishi, Shiraishi, Doi, & Sugahara, 2014). However, studies on the effects of jellyfish collagen on the innate immune response have rarely been reported.…”

Activation of J774.1 murine macrophages by lactate dehydrogenase

“…One is to exclude foreign bodies by phagocytosis, and the other is to present foreign bodies fragmented by phagocytosis to helper T cells as an antigen to connect between the innate and adaptive immune systems (Masuda et al 2006). We previously reported that collagens from jellyfish and bovine Achilles' tendon activate cytokine production by the murine macrophage-like cell line J774.1 cells (Putra et al 2012). Several plant extracts have also been reported to activate macrophages.…”