Effect of cold water-induced stress on immune response, pathology and fertility in mice during Chlamydia muridarum genital infection

Belay, Tesfaye; Woart, Anthony; Graffeo, Vincent

doi:10.1093/femspd/ftx045

Cited by 10 publications

(19 citation statements)

References 68 publications

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“…As shown in Table 1, there are greater numbers of chlamydia inclusion counts in the swabs of stressed mice compared to non-stressed mice, indicating that cold-induced stress increases the intensity of C. muridarum genital infection in mice. Our results are consistent with our previous findings of increased C. muridarum shedding in regions of the genital tract of stressed mice compared to non-stressed mice [59,60].…”

Section: Cold Water-induced Stress Increases the Intensity Of Chlamydsupporting

confidence: 93%

“…We showed previously that repeated exposure of mice to cold water leads to increased norepinephrine and epinephrine production in plasma, spleen, and genital tract lysates that are speculated in increasing the intensity of C. muridarum genital infection [59,60]. In most cases of our findings, the Ct is inversely proportional to the initial copy number and a direct relationship of a higher initial copy number to a lower threshold cycle was obtained.…”

Section: Discussionsupporting

confidence: 65%

“…Application of cold water as a stressor in animal models, including mice, has resulted in changes in immune responses that correlate with the activity of the neuroendocrine system of corticosteroids and catecholamines [55][56][57][58][59][60]. Although stress is implicated as a risk factor for various infections, its effect on chlamydia genital infection is unknown.…”

Section: Trachomatismentioning

confidence: 99%

“…Although stress is implicated as a risk factor for various infections, its effect on chlamydia genital infection is unknown. We have previously shown that cold-water stress induces the production of catecholamines, which may play a critical role in the modulation of the immune system leading to increased intensity of C. muridarum genital infection [59,60]. Furthermore, we have shown that supplement of NE to in vitro exerts an immunosuppressive effect on splenic T cell production of cytokines, decreased C. muridarum shedding in the genital tract of β1Adr/β2Adr KO mice, and severe pathology with a higher rate of infertility in C. muridarum infected mice [60].…”

Section: Trachomatismentioning

confidence: 99%

“…The level of cytokines in supernatants collected from in vitro proliferating CD4+ T cells and bone-marrow derived dendritic cells (BMDCs) of different treatment groups was measured using an ELISA kit (Invitrogen, Camarillo, CA) following the manufacturer's instructions as previously described (59). Briefly, standard samples and test samples were diluted and added to wells in duplicate and incubated as directed.…”

Section: Priming Th1 or Th2 In Vitro Under Polarizing Conditionsmentioning

confidence: 99%

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Modulation of T helper 1 and T helper 2 immune balance in a stress mouse model duringChlamydia muridarumgenital infection

Belay

Martin

Brown

et al. 2019

Preprint

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Running Title: Stress and Chlamydia muridarum genital infection ABSTRACTA mouse model to study the effect of cold-induced stress on Chlamydia muridarum genital infection and immune response has been developed in our laboratory. Our previous results show that cold-induced stress increases the intensity of chlamydia genital infection, but little is known about the effect of cold-induced stress on differentiations and activities of T cell subpopulations and bone marrow derived dendritic cells (BMDCs). The factors that regulate the production of T helper 1 (Th1) or T helper 2 (Th2) cytokines is not clear. The objective of this study was to examine whether cold-induced stress modulates the expression of transcription factors and hallmark cytokines of Th1 and Th2 or differentiation of BMDCs during C. muridarum genital infection in mice. Our results show that mRNA level of beta2-adrenergic receptor (2-AR) compared to 1-AR and 3-AR was high in mixed population of CD4+ T cells and BMDCs.Further, decreased expression of T-bet and low level of interferon-gamma (IFN-) production and increased expression of GATA-3 and interleukin-4 (IL-4) production in CD4+ T of stressed mice was observed. Exposure of BMDCs to feroterol (2-AR agonist) or ICI,118551 ( 2-AR antagonist), respectively, revealed significant stimulation or inhibition of 2-AR in stressed mice. Moreover, co-culturing of mature BMDC and naïve CD4+ T cells resulted in increased production of IL-4, IL-10, and IL-17 in culture supernatants, suggesting that stimulation of 2-AR leads to the increased production of Th2 cytokines. Overall, our results show for the first time that cold-induced stress is able to modulate the pattern of Th1 and Th2 cytokine environment, suggesting that it promotes the differentiation to Th2 rather than Th1 by the overexpression of GATA-3 correlated with elevated production of IL-4, IL-10, in contrast to a low expression of T-bet correlated with less IFN- secretion in the mouse model.

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Section: Cold Water-induced Stress Increases the Intensity Of Chlamydsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 65%

Section: Trachomatismentioning

confidence: 99%

Section: Trachomatismentioning

confidence: 99%

Section: Priming Th1 or Th2 In Vitro Under Polarizing Conditionsmentioning

confidence: 99%

See 3 more Smart Citations

Modulation of T helper 1 and T helper 2 immune balance in a stress mouse model duringChlamydia muridarumgenital infection

Belay

Martin

Brown

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

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Immune response to cold exposure: Role of γδ T cells and TLR2‐mediated inflammation

Vasek,

Holicek,

Galatik

et al. 2024

Eur J Immunol

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The mammalian body possesses remarkable adaptability to cold exposure, involving intricate adjustments in cellular metabolism, ultimately leading to thermogenesis. However, cold‐induced stress can impact immune response, primarily through noradrenaline‐mediated pathways. In our study, we utilized a rat model subjected to short‐term or long‐term mild cold exposure to investigate systemic immune response during the cold acclimation. To provide human relevance, we included a group of regular cold swimmers in our study. Our research revealed complex relationship between cold exposure, neural signaling, immune response, and thermogenic regulation. One‐day cold exposure triggered stress response, including cytokine production in white adipose tissue, subsequently activating brown adipose tissue, and inducing thermogenesis. We further studied systemic immune response, including the proportion of leukocytes and cytokines production. Interestingly, γδ T cells emerged as possible regulators in the broader systemic response, suggesting their possible contribution in the dynamic process of cold adaptation. We employed RNA‐seq to gain further insights into the mechanisms by which γδ T cells participate in the response to cold. Additionally, we challenged rats exposed to cold with the Toll‐like receptor 2 agonist, showing significant modulation of immune response. These findings significantly contribute to understanding of the physiological acclimation that occur in response to cold exposure.

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Severe maternal stress alters placental function, resulting in adipose tissue and liver dysfunction in offspring of mice

Kondo

Ozawa

Satomi

et al. 2023

Molecular and Cellular Endocrinology

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Effect of cold water-induced stress on immune response, pathology and fertility in mice during Chlamydia muridarum genital infection

Cited by 10 publications

References 68 publications

Modulation of T helper 1 and T helper 2 immune balance in a stress mouse model duringChlamydia muridarumgenital infection

Modulation of T helper 1 and T helper 2 immune balance in a stress mouse model duringChlamydia muridarumgenital infection

Immune response to cold exposure: Role of γδ T cells and TLR2‐mediated inflammation

Severe maternal stress alters placental function, resulting in adipose tissue and liver dysfunction in offspring of mice

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