Effect of Cold Plasma on Alternaria Brassicicola Morphology and Seed Germinations

Thammaniphit, Chayaporn; Suwannarat, Sawita; Ruangwong, Khomsan; Srisonphan, Siwapon

doi:10.1109/ieecon48109.2020.229496

Cited by 2 publications

(4 citation statements)

References 15 publications

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“…6 The wall of A. brassicicola spores is relatively thicker and has a complex membrane structure compared with several pathogens, resulting in difficult elimination. 6 Our previous report suggested that after ∼15 min of direct SCP treatment, 45 morphological changes in A. brassicicola spores can be observed. Therefore, in the case of dry treatment, we dropped a ∼50 μL of spore suspension (∼2 × 10 5 spore/mL) on a sterile glass slide and left it to dry under ambient conditions (∼2 h).…”

Section: ■ Results and Discussionmentioning

confidence: 96%

“…Besides, recent investigations have shown that both direct and indirect nonthermal plasma treatment approaches significantly affect the efficiency of seed sterilization. 9,20,39,41,44,45 In direct plasma treatment, the seeds or any biological materials are directly exposed to the area of discharge composed entirely of plasma components (ions, electrons, and different exciting species). In contrast, in indirect treatment, seeds are exposed to nonequilibrium gas outside the plasma discharge region but close enough to benefit from interaction with long-lived radicals.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…In contrast, the electrohydraulic discharge can instantly provide the advantage of short-lived species that interact directly with the target in addition to typical long-lived RONS. Besides, recent investigations have shown that both direct and indirect nonthermal plasma treatment approaches significantly affect the efficiency of seed sterilization. ,,,,, In direct plasma treatment, the seeds or any biological materials are directly exposed to the area of discharge composed entirely of plasma components (ions, electrons, and different exciting species). In contrast, in indirect treatment, seeds are exposed to nonequilibrium gas outside the plasma discharge region but close enough to benefit from interaction with long-lived radicals. ,,, The direct plasma treatment gives stronger antibacterial or microbe inactivation than the indirect plasma treatment because the indirect treatment only delivers to longer-lived or recombined species with lower energy and lower concentrations …”

Section: Resultsmentioning

confidence: 99%

“…brassicicola spores is relatively thicker and has a complex membrane structure compared with several pathogens, resulting in difficult elimination . Our previous report suggested that after ∼15 min of direct SCP treatment, morphological changes in A. brassicicola spores can be observed.…”

Section: Resultsmentioning

confidence: 99%

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Electrohydraulic Streamer Discharge Plasma-Enhanced Alternaria brassicicola Disinfection in Seed Sterilization

Suwannarat

Thammaniphit

Srisonphan

2021

ACS Appl. Mater. Interfaces

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As emerging chemical-free and eco-friendly technologies, nonthermal (gas discharge) plasma and (liquid phase) plasma-activated water (PAW) offer exceptional microbial disinfection solutions for biological, medical, environmental, and agricultural applications. Herein, we present electrohydraulic streamer discharge plasma (ESDP), which combines streamer discharge plasma (SDP) and PAW generated at a gas–liquid interface, to sterilize Chinese kale (Brassica oleracea var. alboglabra) seeds contaminated with Alternaria brassicicola (A. brassicicola). The results showed that the ESDP treatment of A. brassicicola-inoculated seeds provides a ∼75% reduction of A. brassicicola (incident percentage) compared with nontreated seeds. Likewise, the healthy seedling percentage of the plasma-treated seeds was significantly improved to ∼70%, while that of the nontreated seeds remained at ∼15%. A microscopic examination was performed, and it confirmed that ESDP can damage the A. brassicicola spores attached to Chinese kale seeds and lead to severe morphological abnormalities after treatment. Also, an electric field simulation was performed, and it indicated that the strongly localized electric field at the liquid–gas interface on the seed surface boundary had initiated local breakdown of the gas at the air–liquid interface, resulting in exceptional physical–chemical reactions for antimicrobial efficacy beyond typical plasma treatments. Moreover, the optical emission spectra and physicochemical properties (pH, conductivity, and oxidation–reduction potential) showed that inactivation is mainly associated with the reactive oxygen–nitrogen species in the liquid and gas phases. We believe that this work is of great interest when using electrical discharge plasma on liquid interfaces in food, agricultural, and medical industries.

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Section: ■ Results and Discussionmentioning

confidence: 96%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Electrohydraulic Streamer Discharge Plasma-Enhanced Alternaria brassicicola Disinfection in Seed Sterilization

Suwannarat

Thammaniphit

Srisonphan

2021

ACS Appl. Mater. Interfaces

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Applications of nonthermal plasma technology on safety and quality of dried food ingredients

et al. 2020

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Cold plasma technology is an efficient, environmental‐friendly, economic and noninvasive technology; and in recent years these advantages placed this novel technology at the centre of diverse studies for food industry applications. Dried food ingredients including spices, herbs, powders and seeds are an important part of the human diet; and the growing demands of consumers for higher quality and safe food products have led to increased research into alternative decontamination methods. Numerous studies have investigated the effect of nonthermal plasma on dried food ingredients for food safety and quality purposes. This review provides critical review on potential of cold plasma for disinfection of dried food surfaces (spices, herbs and seeds), improvement of functional and rheological properties of dried ingredients (powders, proteins and starches). The review further highlights the benefits of plasma treatment for enhancement of seeds performance and germination yield which could be applied in agricultural sector in near future. Different studies applying plasma technology for control of pathogens and spoilage micro‐organisms and modification of food quality and germination of dried food products followed by benefits and current challenges are presented. However, more systemic research needs to be addressed for successful adoption of this technology in food industry.

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Effect of Cold Plasma on Alternaria Brassicicola Morphology and Seed Germinations

Cited by 2 publications

References 15 publications

Electrohydraulic Streamer Discharge Plasma-Enhanced Alternaria brassicicola Disinfection in Seed Sterilization

Electrohydraulic Streamer Discharge Plasma-Enhanced Alternaria brassicicola Disinfection in Seed Sterilization

Applications of nonthermal plasma technology on safety and quality of dried food ingredients

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